Anti-Anillin抗体(ab5910)
Key features and details
- Goat polyclonal to Anillin
- Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Anillin抗体
参阅全部 Anillin 一抗 -
描述
山羊多克隆抗体to Anillin -
宿主
Goat -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Cow
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免疫原
Synthetic peptide corresponding to Human Anillin aa 1100 to the C-terminus (C terminal). (NP_001271230.1; NP_001271231.1)
Database link: Q9NQW6 -
阳性对照
- ICC/IF: U2OS cells. Flow Cyt (intra): MCF7 cells. IHC-P: Human kidney tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
靶标
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功能
Required for cytokinesis. Essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression. -
组织特异性
Ubiquitously expressed. Present at highest levels in the brain, at high levels in the placenta and testis, at intermediate levels in the intestine, ovary, skeletal muscle and thymus and at lower levels in heart, kidney, liver, lung, pancreas, prostate and spleen. Overexpressed in many tumor types including breast, colorectal, endometrial, hepatic, kidney, lung, ovarian and pancreatic tumors. -
序列相似性
Contains 1 PH domain. -
发展阶段
Expressed in fetal brain, heart, kidney, liver, lung, skeletal muscle, spleen and thymus. In dividing cells expression increases during S and G2 phases, peaks at mitosis and subsequently drops as cells enter G1 phase. -
翻译后修饰
Phosphorylated during mitosis.
Ubiquitinated, and this requires FZR1/CDH1. -
细胞定位
Nucleus. Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Mainly found in the nucleus during interphase. Colocalizes with cortical F-actin upon nuclear envelope breakdown in mitosis and subsequently concentrates in the area of the prospective contractile ring in anaphase. This pattern persists until telophase, when the protein becomes concentrated in the midbody. - Information by UniProt
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数据库链接
- Entrez Gene: 54443 Human
- Entrez Gene: 68743 Mouse
- Omim: 616027 Human
- SwissProt: Q9NQW6 Human
- SwissProt: Q8K298 Mouse
- Unigene: 62180 Human
- Unigene: 282751 Mouse
- Unigene: 453238 Mouse
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别名
- Actin binding protein scraps homolog Drosophila antibody
- Actin binding protein anillin antibody
- Actin-binding protein anillin antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Anillin antibody (ab5910)
ab5910 at 10ug/ml staining Anillin in human kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent antigen retrieval in microwave and in Tris/EDTA buffer (pH 9.0). HRP-staining procedure was used for detection.
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Flow Cytometry (Intracellular) - Anti-Anillin antibody (ab5910)Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) and permeabilized with 0.5% Triton using ab5910 . Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control: Unimmunized goat IgG (black line) fol -
Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody (ab5910)Immunocytochemistry/Immunofluorescence analysis of U2OS cells labelling Anillin with ab5910 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.
Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).
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Immunocytochemistry/ Immunofluorescence - Anti-Anillin antibody (ab5910)This image is courtesy of an Abreview submitted by Manoj B Menonab14404 staining Anillinin Mouse fibroblast cellsby ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with methanol and blocked with 4% BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/25 in PBS + 1% BSA) for 2 hour at 25°C. An Alexa Fluor®488-conjugated Donkey anti-goat polyclonal(1/500) was used as the secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab5910 被引用在 8 文献中.
- Han L et al. Lamin B2 Levels Regulate Polyploidization of Cardiomyocyte Nuclei and Myocardial Regeneration. Dev Cell 53:42-59.e11 (2020). PubMed: 32109383
- Kalebic N et al. Neocortical Expansion Due to Increased Proliferation of Basal Progenitors Is Linked to Changes in Their Morphology. Cell Stem Cell 24:535-550.e9 (2019). PubMed: 30905618
- Luján P et al. PRL-3 disrupts epithelial architecture by altering the post-mitotic midbody position. J Cell Sci 129:4130-4142 (2016). PubMed: 27656108
- Zhou L et al. Anxiety and depression with neurogenesis defects in exchange protein directly activated by cAMP 2-deficient mice are ameliorated by a selective serotonin reuptake inhibitor, Prozac. Transl Psychiatry 6:e881 (2016). WB . PubMed: 27598965
- Stoeckelhuber M et al. Human axillary apocrine glands: proteins involved in the apocrine secretory mechanism. Histol Histopathol 26:177-84 (2011). IHC-P ; Human . PubMed: 21154231
- Stubbs D et al. Neurovascular congruence during cerebral cortical development. Cereb Cortex 19 Suppl 1:i32-41 (2009). PubMed: 19386634
- Bantubungi K et al. Stem cell factor and mesenchymal and neural stem cell transplantation in a rat model of Huntington's disease. Mol Cell Neurosci 37:454-70 (2008). PubMed: 18083596
- Hall PA et al. The septin-binding protein anillin is overexpressed in diverse human tumors. Clin Cancer Res 11:6780-6 (2005). IHC-P ; Human . PubMed: 16203764