AB124734

Anti-Angiotensin II Type 1 Receptor 抗体 [EPR3873]

Name: Angiotensin II Type 1 Receptor抗体[EPR3873] (ab124734)| Abcam中文官网
Brand: Abcam Limited
SKU: AB124734
Price: 1031 CNY
Availability: InStock
Rating: 2 (1 reviews)

Anti-Angiotensin II Type 1 Receptor antibody [EPR3873]

20ul selling size
RabMAb
Recombinant
了解详情

(1 Review)

(55 Publications)

Rabbit Recombinant Monoclonal Angiotensin II Type 1 Receptor antibody. Suitable for I-ELISA, WB and reacts with Recombinant full length protein - Human, Mouse, Rat, Human samples. Cited in 55 publications.

查看别名

AGTR1A, AGTR1B, AT2R1, AT2R1B, AGTR1, Type-1 angiotensin II receptor, AT1AR, AT1BR, Angiotensin II type-1 receptor, AT1 receptor

3 Images

Indirect ELISA - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (AB124734)

I-ELISA

Supplier Data

Indirect ELISA - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (AB124734)

ELISA analysis of Human Angiotensin II Type 1 Receptor recombinant protein at 250 ng/mL with ab124734. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Lab

Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (AB124734)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000,000 dilution.

All lanes:

Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/1000 dilution

Lane 1:

C2C12 (mouse myoblasts myoblast) whole cell lysate at 20 µg

Lane 2:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 41 kDa,36 kDa

false

Exposure time: 140s

Lab

Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (AB124734)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : Lane 1 : 40 seconds - Lane 2 : 80 seconds.

Lane 1:

Western blot - Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] (ab124734) at 1/1000 dilution

Lane 2:

LIF-tag at 1/10000 dilution

All lanes:

LIF tagged Recombinant Human AGTR1 protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 41 kDa

Observed band size: 62 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Angiotensin II Type 1 Receptor antibody [EPR3873] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR3873

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

I-ELISA, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The immunogen used for this product shares 53% homology with Erbin/LAP2. Cross-reactivity with this protein has not been confirmed experimentally.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Angiotensin II Type 1 Receptor also known as AGTR1 AT1 receptor or Angiotensin Receptor 1 is a G protein-coupled receptor with a mass of approximately 45 kDa. This receptor binds to the angiotensin II peptide playing a central role in regulating blood pressure. It is broadly expressed in tissues such as vascular smooth muscle cells cardiac myocytes kidneys and brain. The AT1 receptor mediates vasoconstriction aldosterone secretion and cellular growth through its interaction with angiotensin II impacting cardiovascular functions.

Biological function summary

The Angiotensin II Type 1 Receptor contributes significantly to the regulation of cardiovascular homeostasis. It forms part of a complex signaling system involving G proteins phospholipase C and other downstream effectors. This receptor elicits responses important for maintaining vascular tone and fluid balance. By activating intracellular signaling cascade it plays an essential role in sodium reabsorption and systemic blood pressure maintenance. The receptor's expression and activity represent an important component in cardiovascular function.

Pathways

The AT1 receptor participates actively in the renin-angiotensin system (RAS) and is a significant player in the regulation of blood pressure. It interacts with angiotensin converting enzyme (ACE) and angiotensin II within this pathway. Additionally the receptor is linked to the mitogen-activated protein kinase (MAPK) pathway which further influences cell proliferation and vascular remodeling. These interactions highlight the receptor's involvement in vascular smooth muscle cell function and the broader implications for cardiovascular health.

The Angiotensin II Type 1 Receptor is closely associated with conditions such as hypertension and heart failure. Dysfunction or overactivity of this receptor contributes to elevated blood pressure and compromised cardiac function. The AT1 receptor's connection to ACE highlights its therapeutic relevance as many antihypertensive treatments target this interaction. By modulating the receptor's activity these treatments aim to alleviate symptoms and reduce the risk of cardiovascular events.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Receptor for angiotensin II, a vasoconstricting peptide, which acts as a key regulator of blood pressure and sodium retention by the kidney (PubMed : 15611106, PubMed : 1567413, PubMed : 25913193, PubMed : 26420482, PubMed : 30639100, PubMed : 32079768, PubMed : 8987975). The activated receptor in turn couples to G-alpha proteins G(q) (GNAQ, GNA11, GNA14 or GNA15) and thus activates phospholipase C and increases the cytosolic Ca(2+) concentrations, which in turn triggers cellular responses such as stimulation of protein kinase C (PubMed : 15611106).. (Microbial infection) During SARS coronavirus-2/SARS-CoV-2 infection, it is able to recognize and internalize the complex formed by secreted ACE2 and SARS-CoV-2 spike protein through DNM2/dynamin 2-dependent endocytosis.

See full target information AGTR1

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文献 (55)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:35479 PubMed41073421

2025

Obesity exacerbates experimental colitis due to the activation of the local renin-angiotensin system in the colon.

Applications

Unspecified application

Species

Unspecified reactive species

Maomao Pan,Qiang Xu,Linjing Huang,Yuanyuan Bai,Xue Li,Yibing Tang

Scientific reports 15:915 PubMed39762506

2025

Renal glomerular and tubular injury in the offspring of the preeclampsia-like syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Wang,Hao Wang,Huiqing Lu,Ji Ma,Wei Wu,Yinan Wang,Bo Ma,Hao Zhu,Rong Hu

NPJ Parkinson's disease 10:220 PubMed39548112

2024

Cannabinoid regulation of angiotensin II-induced calcium signaling in striatal neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael Rivas-Santisteban,Ana Muñoz,Jaume Lillo,Iu Raïch,Ana I Rodríguez-Pérez,Gemma Navarro,José L Labandeira-García,Rafael Franco

Neurobiology of disease 202:106729 PubMed39515529

2024

Neuronal ACE1 knockout disrupts the hippocampal renin angiotensin system leading to memory impairment and vascular loss in normal aging.

Applications

Unspecified application

Species

Unspecified reactive species

Sohee Jeon,Miranda A Salvo,Alia O Alia,Jelena Popovic,Mitchell Zagardo,Sidhanth Chandra,Malik Nassan,David Gate,Robert Vassar,Leah K Cuddy

Dose-response : a publication of International Hormesis Society 22:15593258241298045 PubMed39506979

2024

Salidroside Pre-Treatment Inhibits Hypertensive Renal Injury and Fibrosis Through Inhibiting Wnt/β-Catenin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhu,Liang Li,Yuting Luan,Ziqing Zhang,Yi Wang,Zhenyu Xu

Phytotherapy research : PTR 38:5918-5929 PubMed39410864

2024

α-Mangostin Attenuates Blood Pressure and Reverses Vascular Remodeling by Balancing ACE/AT1R and ACE2/Ang-(1-7)/MasR Axes in Ang II-Infused Hypertensive Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Qi Xue,Chu-Hao Liu,Dong-Yan Zhang,Ming-Xuan Li,Yan Li

Molecular medicine (Cambridge, Mass.) 30:78 PubMed38844873

2024

Wogonin upregulates SOCS3 to alleviate the injury in Diabetic Nephropathy by inhibiting TLR4-mediated JAK/STAT/AIM2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yufeng Liu,Mengbi Zhang,Lu Zeng,Yanhong Lai,Songzhao Wu,Xiaoyan Su

Frontiers in neuroscience 18:1401530 PubMed38741786

2024

Sleep deprivation reduces the baroreflex sensitivity through elevated angiotensin (Ang) II subtype 1 receptor expression in the nucleus tractus solitarii.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Feng Liu,Yu-Wan Wang,Jia-Cen Sun,Yang-Kai Wang,Xing Tan,Wei-Zhong Wang

SAGE open medicine 11:20503121231220813 PubMed38144881

2023

Comparison of the antihypertensive effects of folic acid and resveratrol in spontaneously hypertensive rats combined with hyperhomocysteinemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Lu,Lihua Zhang,Chunli Wang,Chunbo Gong

Physiological research 72:597-606 PubMed38015759

2023

MicroRNA-206 Contributes to the Progression of Preeclampsia by Suppressing the Viability and Mobility of Trophocytes via the Inhibition of AGTR1.

Applications

Unspecified application

Species

Unspecified reactive species

W Mo,J Jin,X Wang,W Luan,J Yan,X Long

View all publications

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Anti-Angiotensin II Type 1 Receptor 抗体 [EPR3873]