Anti-Angiopoietin-like 4/ANGPTL4抗体(ab2920)
Key features and details
- Rabbit polyclonal to Angiopoietin-like 4/ANGPTL4
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-Angiopoietin-like 4/ANGPTL4抗体
参阅全部 Angiopoietin-like 4/ANGPTL4 一抗 -
描述
兔多克隆抗体to Angiopoietin-like 4/ANGPTL4 -
宿主
Rabbit -
特异性
Detects fasting-induced adipose factor (FIAF) from rat adipose tissue. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide corresponding to Mouse Angiopoietin-like 4/ANGPTL4 aa 80-94 (N terminal).
Sequence:CQGPKGKDAPFKDSE
(Peptide available asab4936) -
阳性对照
- Rat adipose tissue extracts.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Adipose tissue is an energy reserve in animals, and in nondomestic species it is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins that help regulate whole body metabolism, such as leptin and Acrp30. Fasting causes significant changes in nutrient metabolism and many of these changes are controlled by transcription factors that regulate rate-limiting enzymes. An important transcription factor that mediates metabolic processes induced by fasting is peroxisome proliferator-activated receptor alpha (PPAR alpha). PPAR alpha has recently been reported to target a novel gene encoding the secreted protein FIAF (fasting-induced adipose factor). FIAF is expressed predominantly in adipose tissue and is strongly upregulated in white adipose tissue and the liver under fasting conditions. Initial studies suggest that FIAF represents a novel endocrine signal helping to regulate metabolism, especially under fasting conditions. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use a concentration of 2 µg/ml.
By Western blot, this antibody detects an ~50 kDa protein representing FIAF from rat adipose tissue extracts. |
| 说明 |
|---|
|
WB
Use a concentration of 2 µg/ml. By Western blot, this antibody detects an ~50 kDa protein representing FIAF from rat adipose tissue extracts. |
靶标
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功能
Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation. -
组织特异性
Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney. -
序列相似性
Contains 1 fibrinogen C-terminal domain. -
翻译后修饰
N-glycosylated. -
细胞定位
Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4. - Information by UniProt
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数据库链接
- Entrez Gene: 362850 Rat
- SwissProt: Q6TMA8 Rat
- Unigene: 119611 Rat
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别名
- Angiopoietin like 4 antibody
- Angiopoietin related protein 4 antibody
- Angiopoietin-like protein 4 antibody
see all
图片
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Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab2920)Western blot on FIAF in rat adipose tissue extract using ab2920. Western blot on FIAF in rat adipose tissue extract using ab2920.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab2920 被引用在 6 文献中.
- Zhou S et al. Adipocytes induce the resistance of ovarian cancer to carboplatin through ANGPTL4. Oncol Rep 44:927-938 (2020). PubMed: 32705217
- Plaza A et al. Cholecystokinin is involved in triglyceride fatty acid uptake by rat adipose tissue. J Endocrinol 236:137-150 (2018). PubMed: 29339381
- Rodrigues T et al. Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance. Sci Rep 7:1698 (2017). WB ; Rat . PubMed: 28490763
- Blanchard PG et al. Peroxisome proliferator-activated receptor γ activation favours selective subcutaneous lipid deposition by coordinately regulating lipoprotein lipase modulators, fatty acid transporters and lipogenic enzymes. Acta Physiol (Oxf) 217:227-39 (2016). PubMed: 26918671
- Villa L et al. Effects and mechanisms of angiotensin II receptor blockade with telmisartan in a normotensive model of mesangioproliferative nephritis. Nephrol Dial Transplant : (2011). PubMed: 21414972
- Koliwad SK et al. Angiopoietin-like 4 (ANGPTL4, fasting-induced adipose factor) is a direct glucocorticoid receptor target and participates in glucocorticoid-regulated triglyceride metabolism. J Biol Chem 284:25593-601 (2009). WB ; Mouse . PubMed: 19628874