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Cardiovascular Angiogenesis Inhibitors

Anti-Angiopoietin-like 4/ANGPTL4抗体(ab2920)

  • Datasheet
  • SDS
Reviews (1)Q&A (4)References (6)

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Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab2920)

    Key features and details

    • Rabbit polyclonal to Angiopoietin-like 4/ANGPTL4
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Angiopoietin-like 4/ANGPTL4抗体
      参阅全部 Angiopoietin-like 4/ANGPTL4 一抗
    • 描述

      兔多克隆抗体to Angiopoietin-like 4/ANGPTL4
    • 宿主

      Rabbit
    • 特异性

      Detects fasting-induced adipose factor (FIAF) from rat adipose tissue.
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Rat
    • 免疫原

      Synthetic peptide corresponding to Mouse Angiopoietin-like 4/ANGPTL4 aa 80-94 (N terminal).
      Sequence:

      CQGPKGKDAPFKDSE


      (Peptide available as ab4936)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 阳性对照

      • Rat adipose tissue extracts.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • Primary antibody说明

      Adipose tissue is an energy reserve in animals, and in nondomestic species it is strictly regulated. Adipose cells produce and secrete numerous physiologically important proteins that help regulate whole body metabolism, such as leptin and Acrp30. Fasting causes significant changes in nutrient metabolism and many of these changes are controlled by transcription factors that regulate rate-limiting enzymes. An important transcription factor that mediates metabolic processes induced by fasting is peroxisome proliferator-activated receptor alpha (PPAR alpha). PPAR alpha has recently been reported to target a novel gene encoding the secreted protein FIAF (fasting-induced adipose factor). FIAF is expressed predominantly in adipose tissue and is strongly upregulated in white adipose tissue and the liver under fasting conditions. Initial studies suggest that FIAF represents a novel endocrine signal helping to regulate metabolism, especially under fasting conditions.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Cardiovascular
      • Angiogenesis
      • Inhibitors
      • Cardiovascular
      • Angiogenesis
      • Growth Factors
      • Other
      • Cardiovascular
      • Hypoxia
      • Hypoxia-Regulated
      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Cholesterol Metabolism
      • Cardiovascular
      • Angiogenesis
      • Angiogenic Factors
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of lipids and lipoproteins
      • Cancer
      • Cancer Metabolism
      • Response to hypoxia
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipid metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Cholesterol Metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Hypoxia
      • Metabolism
      • Types of disease
      • Cancer

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab2920于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    Use a concentration of 2 µg/ml.

    By Western blot, this antibody detects an ~50 kDa protein representing FIAF from rat adipose tissue extracts.

    说明
    WB
    Use a concentration of 2 µg/ml.

    By Western blot, this antibody detects an ~50 kDa protein representing FIAF from rat adipose tissue extracts.

    靶标

    • 功能

      Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorgenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM). The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation.
    • 组织特异性

      Expressed at high levels in the placenta, heart, liver, muscle, pancreas and lung but expressed poorly in the brain and kidney.
    • 序列相似性

      Contains 1 fibrinogen C-terminal domain.
    • 翻译后修饰

      N-glycosylated.
    • 细胞定位

      Secreted. Secreted > extracellular space > extracellular matrix. The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4.
    • Target information above from: UniProt accession Q9BY76 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 362850 Rat
      • SwissProt: Q6TMA8 Rat
      • Unigene: 119611 Rat
      • 别名

        • Angiopoietin like 4 antibody
        • Angiopoietin related protein 4 antibody
        • Angiopoietin-like protein 4 antibody
        • Angiopoietin-related protein 4 antibody
        • ANGL4_HUMAN antibody
        • ANGPT L2 antibody
        • ANGPT L4 antibody
        • ANGPTL2 antibody
        • Angptl4 antibody
        • ARP4 antibody
        • Fasting induced adipose factor antibody
        • FIAF antibody
        • HARP antibody
        • Hepatic angiopoietin related protein antibody
        • Hepatic fibrinogen/angiopoietin related protein antibody
        • Hepatic fibrinogen/angiopoietin-related protein antibody
        • HFARP antibody
        • NL2 antibody
        • Peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin related protein antibody
        • PGAR antibody
        • pp1158 antibody
        • PPARG angiopoietin related protein antibody
        • PSEC0166 antibody
        • TGQTL antibody
        • UNQ171 antibody
        • Weakly similar to angiopoietin 1 [H.sapiens] antibody
        see all

      图片

      • Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab2920)
        Western blot - Anti-Angiopoietin-like 4/ANGPTL4 antibody (ab2920)
        Western blot on FIAF in rat adipose tissue extract using ab2920. Western blot on FIAF in rat adipose tissue extract using ab2920.

      实验方案

      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (6)

      发表研究结果有使用 ab2920?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab2920 被引用在 6 文献中.

      • Zhou S  et al. Adipocytes induce the resistance of ovarian cancer to carboplatin through ANGPTL4. Oncol Rep 44:927-938 (2020). PubMed: 32705217
      • Plaza A  et al. Cholecystokinin is involved in triglyceride fatty acid uptake by rat adipose tissue. J Endocrinol 236:137-150 (2018). PubMed: 29339381
      • Rodrigues T  et al. Methylglyoxal-induced glycation changes adipose tissue vascular architecture, flow and expansion, leading to insulin resistance. Sci Rep 7:1698 (2017). WB ; Rat . PubMed: 28490763
      • Blanchard PG  et al. Peroxisome proliferator-activated receptor γ activation favours selective subcutaneous lipid deposition by coordinately regulating lipoprotein lipase modulators, fatty acid transporters and lipogenic enzymes. Acta Physiol (Oxf) 217:227-39 (2016). PubMed: 26918671
      • Villa L  et al. Effects and mechanisms of angiotensin II receptor blockade with telmisartan in a normotensive model of mesangioproliferative nephritis. Nephrol Dial Transplant : (2011). PubMed: 21414972
      • Koliwad SK  et al. Angiopoietin-like 4 (ANGPTL4, fasting-induced adipose factor) is a direct glucocorticoid receptor target and participates in glucocorticoid-regulated triglyceride metabolism. J Biol Chem 284:25593-601 (2009). WB ; Mouse . PubMed: 19628874

      客户评价及客户问答

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      Western blot abreview for Anti-Angiopoietin-like 4/ANGPTL4 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - other (white fat tissue)
      Gel Running Conditions
      Non-reduced Denaturing (12.5%)
      Loading amount
      30 µg
      Specification
      white fat tissue
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Jan 22 2014

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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