AB117776

Anti-Angiomotin抗体

Name: Angiomotin抗体 (ab117776)| Abcam中文官网
Brand: Abcam Limited
SKU: AB117776
Price: 4829 CNY
Availability: InStock

Anti-Angiomotin antibody

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(1 Publication)

Rabbit Polyclonal Angiomotin antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human AMOT aa 1000 to C-terminus.

查看别名

KIAA1071, AMOT, Angiomotin

2 Images

Unknown

Immunoprecipitation - Anti-Angiomotin antibody (AB117776)

Detection of Angiomotin by Western Blot of Immunprecipitate.
ab117776 at 1µg/ml staining Angiomotin in 293T whole cell lysate immunoprecipitated using ab117776 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 1 second.

All lanes:

Immunoprecipitation - Anti-Angiomotin antibody (ab117776)

Predicted band size: 118 kDa

false

Unknown

Western blot - Anti-Angiomotin antibody (AB117776)

All lanes:

Western blot - Anti-Angiomotin antibody (ab117776) at 0.04 µg/mL

Lane 1:

293T whole cell lysates at 50 µg

Lane 2:

293T whole cell lysates at 15 µg

Lane 3:

HeLa whole cell lysates at 50 µg

Predicted band size: 118 kDa

true

Exposure time: 3s

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IP

applications

免疫原

Synthetic Peptide within Human AMOT aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q4VCS5

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

ab117776 was affinity purified using an epitope specific to Angiomotin immobilized on solid support.

存储溶液

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Angiomotin also known as AMOT is a protein that has significant roles in various cellular processes. It has a molecular mass of approximately 130 kDa. Angiomotin is widely expressed across several tissues including endothelial cells where it functions as a regulator of cell migration and angiogenesis. It interacts directly with actin filaments affecting cell shape and movement therefore playing a role in the structural organization of cells.

Biological function summary

Angiomotin influences endothelial cell behavior through its involvement in cellular proliferation and mobility. Angiomotin forms part of a complex network by binding with other proteins such as Patj and LATS1/2 aiding in the control of the Hippo signaling pathway. This activity regulates organ size and cellular growth impacting tissue architecture and repair mechanisms in the body.

Pathways

Angiomotin's interactions contribute notably to the Hippo and MAPK signaling pathways. Within the Hippo pathway it aid the suppression of excessive cell proliferation by keeping the activity of YAP and TAZ in check. In the context of the MAPK pathway Angiomotin regulates signals that influence cell division and differentiation. The protein influences cellular outcomes by cooperating with other proteins like YAP a transcriptional coactivator often implicated in organ size and cancer.

Angiomotin is associated with various vascular conditions and cancers. Altered expression or dysregulation can contribute to pathological angiogenesis which plays a role in tumor progression and metastasis. In cancer Angiomotin impacts the Hippo pathway's ability to control YAP's oncogenic potential affecting cellular proliferation and tumor development. Understanding these interactions offers potential therapeutic targets for disorders linked to abnormal cell growth and vascular anomalies.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a central role in tight junction maintenance via the complex formed with ARHGAP17, which acts by regulating the uptake of polarity proteins at tight junctions. Appears to regulate endothelial cell migration and tube formation. May also play a role in the assembly of endothelial cell-cell junctions. Repressor of YAP1 and WWTR1/TAZ transcription of target genes, potentially via regulation of Hippo signaling-mediated phosphorylation of YAP1 which results in its recruitment to tight junctions (PubMed : 21205866).

See full target information AMOT

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 40:523-536.e6 PubMed28350986

2017

Flow-Dependent Endothelial YAP Regulation Contributes to Vessel Maintenance.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroyuki Nakajima,Kimiko Yamamoto,Sobhika Agarwala,Kenta Terai,Hajime Fukui,Shigetomo Fukuhara,Koji Ando,Takahiro Miyazaki,Yasuhiro Yokota,Etienne Schmelzer,Heinz-Georg Belting,Markus Affolter,Virginie Lecaudey,Naoki Mochizuki

View all publications

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Anti-Angiomotin抗体

Anti-Angiomotin antibody

Immunoprecipitation - Anti-Angiomotin antibody (AB117776)

Western blot - Anti-Angiomotin antibody (AB117776)