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重组RabMAb

重组Anti-Androgen Receptor (AR-V7 specific)抗体[EPR15656-290] - BSA and Azide free (ab273511)

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Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] - BSA and Azide free (ab273511)
  • Flow Cytometry (Intracellular) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] - BSA and Azide free (ab273511)
  • Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] - BSA and Azide free (ab273511)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15656-290] to Androgen Receptor (AR-V7 specific) - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 APC PE Unconjugated

概述

  • 产品名称

    Anti-Androgen Receptor (AR-V7 specific)抗体[EPR15656-290] - BSA and Azide free
    参阅全部 Androgen Receptor (AR-V7 specific) 一抗

  • 描述

    兔单克隆抗体[EPR15656-290] to Androgen Receptor (AR-V7 specific) - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, ICC/IFmore details
    不适用于: IHC-P

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: 22Rv1 whole cell lysate. ICC/IF: 22Rv1 cells. Flow Cyt (intra): 22Rv1 cells.

  • 常规说明

    ab273511 is the carrier-free version of ab273500.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • 存储溶液

    pH: 7.2
    Constituent: PBS

  • 无载体

    是
  • Concentration information loading...

  • 纯度

    Protein A purified

  • 克隆

    单克隆

  • 克隆编号

    EPR15656-290

  • 同种型

    IgG

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab273511于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 99 kDa).
ICC/IF
Use at an assay dependent concentration.
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 80 kDa (predicted molecular weight: 99 kDa).
ICC/IF
Use at an assay dependent concentration.
应用说明
Is unsuitable for IHC-P.

靶标

  • 相关性

    Belongs to the nuclear hormone receptor family. Contains nuclear receptor DNA-binding domain. Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Transcription factor activity is modulated by bound coactivator and corepressor proteins. This target is a splice variant of Androgen receptor (UniProt P10275) lacking the C-terminal androgen binding site.

  • 细胞定位

    Nuclear

  • 数据库链接

    • 别名

      • Androgen receptor splice variant 3 antibody
      • AR-V7 antibody
      • AR-V7(AR3) antibody
      • AR3 antibody
      • ARv7 antibody
      see all

    图片

    • Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] - BSA and Azide free (ab273511)
      Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] - BSA and Azide free (ab273511)

      Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 22Rv1 cells labelling Androgen Receptor (AR-V7 specific) with ab273500 at 1/50 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing nuclear and weakly cytoplasmic staining in 22RV1 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273500).

    • Flow Cytometry (Intracellular) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] - BSA and Azide free (ab273511)
      Flow Cytometry (Intracellular) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] - BSA and Azide free (ab273511)

      Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized 22Rv1 (human prostate carcinoma epithelial cell) cells labelling Androgen Receptor (AR-V7 specific) with ab273500 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273500). 

       

       

    • Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] - BSA and Azide free (ab273511)
      Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656-290] - BSA and Azide free (ab273511)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab273511？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab273511 尚未被引用在任何文献中。

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