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AB133273

重组Anti-Androgen Receptor抗体[EPR1535(2)]

Anti-Androgen Receptor antibody [EPR1535(2)]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • Lab Essentials
  • 了解详情

5

(11 Reviews)

|

(175 Publications)

Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) is a rabbit monoclonal antibody detecting Androgen Receptor in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 120 publications

查看别名

DHTR, NR3C4, AR, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4

27 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Immunohistochemical analysis of formalin fixed paraffin embedded human prostate cancer labelling Androgen Receptor with ab133273 at a concentration of 0.1 µg/ml. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with a Bond™ Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20 mins. ab133273 Anti-Androgen Receptor antibody [EPR1535(2)] was incubated for 30 mins at room temperature. Sections were counterstained with Hematoxylin. Image inset shows absence of staining in secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Immunohistochemical analysis of formalin fixed paraffin embedded human prostate cancer labelling Androgen Receptor with ab133273 at a concentration of 0.1 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab133273 Anti-Androgen Receptor antibody [EPR1535(2)] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Formalin-fixed, paraffin-embedded tissues labeling Androgen Receptor with ab133273. Left : Tumor from orthotopic injection of PTEN KD/TE cells. Right : Human postate cancer tissue.

From Figure 3 of Shao et al/

Shao et al Oncotarget. 2018 Mar 6; 9(18) : 14456–14471.Published online 2018 Feb 12. doi : 10.18632/oncotarget.24470

Reproduced under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0).

shao et al Oncotarget. 2018 Mar 6; 9(18): 14456–14471. Published online 2018 Feb 12. doi: 10.18632/oncotarget.24470

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Immunohistochemical analysis of paraffin-embedded human prostate tissue labelling Androgen Receptor using unpurified ab133273, at1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Immunofluorescent staining of LnCAP cells labelling Androgen Receptor using unpurified ab133273, at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Unpurified ab133273 showing negative staining in Normal liver tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Unpurified ab133273 showing negative staining in Normal colon tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Unpurified ab133273 showing positive staining in Endometrial carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Immunohistochemical analysis of paraffin-embedded human prostatic adenocarcinoma tissue labelling Androgen Receptor using unpurified ab133273 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Unpurified ab133273 showing negative staining in Colonic adenocarcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Unpurified ab133273 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling Androgen receptor with purified ab133273 at 1 : 100 dilution (1.3μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Unpurified ab133273 showing negative staining in Normal tonsil tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostatic hyperplasia tissue sections labeling Androgen Receptor with Purified ab133273 at 1 : 500 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Unpurified ab133273 showing negative staining in Ovarian carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Texas red (Tx-Red) indirect immunofluorescent detection of Androgen Receptor using ab133273 in (A) EP156T-AR, (B) 957E/hTERT-AR and (C) LNCaP cells. The cells were treated with ± 1 nM R1881 for 24 hours.

Image from Azeem W et al., PLoS One. 2017;12(6):e0177861. Fig 6.; doi: 10.1371/journal.pone.0177861. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Unpurified ab133273 showing positive staining in Prostatic carcinoma T3 tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Unpurified ab133273 showing positive staining in Breast carcinoma tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Unpurified ab133273 showing negative staining in Normal breast tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue sections labeling Androgen Receptor with Purified ab133273 at 1 : 500 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse testis tissue sections labeling Androgen Receptor with Purified ab133273 at 1 : 500 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • WB

Lab

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Blocking/Diluting buffer - 5% NFDM/TBST

The androgen receptor variant band detected in 22RV1 cells is reported by PMID : 22315407.

We recommend you to try both RIPA and 1%SDS Hot lysis preparation methods to get desired bands.

All lanes:

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) at 1/20000 dilution

Lane 1:

LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method at 1/15 dilution

Lane 2:

LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method at 1/15 dilution

Lane 3:

22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in RIPA lysis method at 1/15 dilution

Lane 4:

22Rv1 (Human prostate carcinoma epithelial cell) whole cell lysates prepared in 1%SDS Hot lysis method at 1/15 dilution

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 99 kDa

Observed band size: 120 kDa

false

Exposure time: 10s

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • WB

Lab

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Blocking/Diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) at 1/1000 dilution

All lanes:

LNCaP (Human prostate carcinoma epithelial cell) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 99 kDa

Observed band size: 110 kDa

false

Exposure time: 3s

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • WB

Lab

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Different batches of ab133273 were tested on LNCaP (Human prostate carcinoma epithelial cell) lysate at 1.0 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 120 kDa.

All lanes:

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273)

Predicted band size: 99 kDa

false

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • WB

Lab

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) at 1/5000 dilution

Lane 1:

LNCaP (Human prostate carcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Rat prostate lysates at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 146 kDa,99 kDa

Observed band size: 160 kDa

false

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • WB

Lab

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Blocking/Diluting buffer : 5% NFDM/TBST

Loading Control : Rabbit monoclonal [EPR16891] to GAPDH (ab181602)

All lanes:

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) at 1/1000 dilution

Lane 1:

Mouse testis lysate at 20 µg

Lane 2:

Rat testis lysate at 20 µg

Lane 3:

Mouse liver lysate at 20 µg

Lane 4:

Rat liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 99 kDa

Observed band size: 110 kDa

false

Exposure time: 20s

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)
  • WB

Lab

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (AB133273)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) at 1/5000 dilution

All lanes:

Mouse prostate lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 99 kDa

false

不同偶联物与剂型 (6)

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Androgen Receptor antibody [EPR1535(2)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Androgen Receptor antibody [EPR1535(2)]

  • HRP

    HRP Anti-Androgen Receptor antibody [EPR1535(2)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Androgen Receptor antibody [EPR1535(2)]

  • Carrier free

    Anti-Androgen Receptor antibody [EPR1535(2)] - BSA and Azide free

  • Carrier free

    Anti-Androgen Receptor antibody [EPR1535(2)] - Low endotoxin, Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR1535(2)

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human

应用

ICC/IF, WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p><strong>For unpurified use at 1/100-1/250</strong></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p><strong>For unpurified use at 1:1000</strong></p><p>For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or <a href=\"../../technical-resources/protocols/lysate-preparation-for-western-blot\" tabindex=\"-1\">here.</a></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p><strong>For unpurified use at 1/100-1/250</strong></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p><strong>For unpurified use at 1:1000</strong></p><p>For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or <a href=\"../../technical-resources/protocols/lysate-preparation-for-western-blot\" tabindex=\"-1\">here.</a></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p><strong>For unpurified use at 1/100-1/250</strong></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p><strong>For unpurified use at 1:1000</strong></p><p>For Lysate preparation protocol, please refer to the protocol book in the protocol section and/or <a href=\"../../technical-resources/protocols/lysate-preparation-for-western-blot\" tabindex=\"-1\">here.</a></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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产品详情

Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-P, WB in human, mouse, rat samples.

Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) has been cited over 124 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) has 11 independent reviews from customers.

Anti-Androgen Receptor antibody [EPR1535(2)] (ab133273) specifically detects Androgen Receptor (UniProt ID: P10275; Molecular weight: 99kDa) and is sold in 100 µL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR1535(2) - ab271891.

Antibody clone EPR1535(2) is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, HRP, Alexa Fluor® 555 (ab194194, ab194195, ab194196, ab275124).

The androgen receptor (AR)/DHTR plays a crucial role in the development and progression of various cancers, including breast cancer and prostate cancer. Utilizing our highly-validated AR antibody, can significantly enhance the detection and study of AR expression. Research indicates that AR is a promising therapeutic target for treating hormone-dependent cancers, offering potential for improved patient outcomes.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The androgen receptor (AR) also known as NR3C4 functions mechanically as a type of nuclear receptor that is activated by binding androgens including testosterone and dihydrotestosterone. The AR has a molecular weight of approximately 110 kDa. It is expressed in various tissues notably in the prostate skeletal muscle and certain areas of the brain. Within cells AR is usually located in the cytoplasm when unbound and translocates to the nucleus upon activation by its ligands.
Biological function summary

AR interacts with androgen hormones to regulate gene expression. It forms a complex with coactivators and corepressors to modulate transcription of target genes involved in male sexual development and reproductive function. AR plays an important role in the development and maintenance of male characteristics and reproductive activity. Its expression impacts cellular processes such as proliferation differentiation and apoptosis.

Pathways

AR is intricately involved in the androgen signaling pathway which affects the regulation of genes related to male sexual characteristics. It also plays a part in the PI3K/Akt pathway influencing cell growth and survival. The AR pathway interacts with proteins such as estrogen receptors to mediate cross-talk between hormonal pathways highlighting its broad biological impacts.

AR has a significant connection to prostate cancer where it drives tumor growth and progression. Mutations and overexpression of the androgen receptor are often observed in prostate cancer cells. Another condition linked to AR is androgen insensitivity syndrome where mutations in the AR gene result in partial or complete inability of cells to respond to androgenic hormones. In these conditions AR antagonists like bicalutamide are often used as treatment inhibiting the receptor's activity to manage disease progression.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed : 19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed : 20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3.. Isoform 3. Lacks the C-terminal ligand-binding domain and therefore constitutively regulates the transcription of a specific set of canonical AR-target genes, including PSA/KLK3 and TMPRSS2, independently of steroid hormones (PubMed : 19244107, PubMed : 25008967). However, some genes are differentially regulated by full-length AR (isoform 1) and isoform 3. Isoform 3-specific target genes may be regulated independently of FOXA1 expression (PubMed : 25008967).. Isoform 4. Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
See full target information AR
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文献 (175)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 57:2468-2481 PubMed41044247

2025

Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Luo,Zhixiang Chen,Yuanyuan Qiao,Jean Ching-Yi Tien,Eleanor Young,Rahul Mannan,Somnath Mahapatra,Rupam Bhattacharyya,Lanbo Xiao,Tongchen He,Sanjana Eyunni,Yuping Zhang,Yang Zheng,Fengyun Su,Xuhong Cao,Rui Wang,Yunhui Cheng,Rithvik Seri,James George,Miriam Shahine,Stephanie J Miner,Matthew G Rees,Melissa M Ronan,Jennifer A Roth,Ulka Vaishampayan,Mi Wang,Shaomeng Wang,Abhijit Parolia,Arul M Chinnaiyan

Frontiers in immunology 16:1609340 PubMed40873563

2025

Clonal divergence and genomic meltdown in prostatic pleomorphic giant cell adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoshi Ma,Kun Chen,Jing Zhang,Liming Liu,Jiping Luo,Kaipeng Huang,Hongying Zhang,Danni Liu,Jizhou Gou,Changyin Feng,Xia Zhao,Wanying Li,Lipeng Chen,Li Yin,Xianlin Meng,Zhiqiang Cheng

Cancer immunology, immunotherapy : CII 74:268 PubMed40650767

2025

TGF-β3 promotes vascular normalization of prostate cancer to potentiate immunotherapy and chemotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Qiliang Teng,Niu Wang,Hanqi Lei,Tongyu Tong,Yupeng Guan,Mengjun Huang,Fei Cao,Bin Xu,Jia Yang,Yimian Huo,Wenping Chen,Ran Bi,Xuanqi Wang,Zhenyu Wang,Fu-Ying Tian,Bo Zhao,Jun Pang

Nature communications 16:6451 PubMed40651942

2025

Androgen receptor promotes arachidonic acid metabolism and angiogenic microenvironment in AFP-negative hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhilong Lin,Xiaofei Liu,Houwei Wang,Shumin Li,Ziqiang Miao,Jing Yang,Yuting Zhang,Kai Lei,Yifan Wu,Youmei Kang,Ruoyin Zheng,Zonglin Xie,Yixi Wen,Xiaoxue Ren,Chunxiao Liu,Alfred Sze-Lok Cheng,Yubin Xie,Shuling Chen,Ming Kuang,Sui Peng,Zhenwei Peng,Zihao Dai

Frontiers in immunology 16:1602579 PubMed40612952

2025

Expression patterns of estrogen and androgen receptors in NSCLC patients according to the PD-L1 profile.

Applications

Unspecified application

Species

Unspecified reactive species

Vianey Rodriguez-Lara,Gala Cortés-Ramírez,Itzel Amayrani Angeles-Torres,Jeronimo Rodriguez-Cid,Sally María Luisa Pedraza-Reyes,Maria Rosa Avila-Costa,José Luis Ordoñez-Librado,Marco Cerbón

Inflammation : PubMed40504462

2025

AR Deficiency Protects Against Sepsis-Induced Acute Lung Injury by Inhibiting Macrophage M1 Polarization and Inflammatory Cytokine Secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowan Wang,Di Yin,Jiayan Sun,Linna Zhang,Yuheng Ye,Qiang Guo

Biomedicines 13: PubMed40426904

2025

Differential Effects of Canonical Androgens and 11-Ketotestosterone on Reproductive Phenotypes and Folliculogenesis in Mouse Model of PCOS.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ru Tsai,Yen-Nung Liao,Cheng-Ju Tsai,Yu-Ang Lee,Shih-Min Hsia,Kuo-Chung Lan,Hong-Yo Kang

Frontiers in pharmacology 16:1558916 PubMed40201699

2025

An active ingredient from the combination of Corydalis Rhizoma and Paeoniae Radix Alba relieves chronic compression injury-induced pain in rats by ameliorating AR/Mboat2-mediated ferroptosis in spinal cord neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Ze-Ming Wang,Xiao-Hong Wei,Gui-Yang Xia,Lin-Nan Zhou,Jin-Yu Li,Sheng Lin

Anticancer research 45:883-896 PubMed40037856

2025

DNA Ligase 4 Inhibition Sensitizes Prostate Cancer to Immune Checkpoint Blockade .

Applications

Unspecified application

Species

Unspecified reactive species

Jianchun Wu,Angelica M Lagunas,David L Crowe

Biology 14: PubMed40001891

2025

Androgen Receptor Expression Governs the Seasonal Inhibition of Testicular Development and Subsequent Recovery in .

Applications

Unspecified application

Species

Unspecified reactive species

Yaqi Ying,Lewen Wang,Dawei Wang,Ning Li,Ying Song,Xiaohui Liu
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替代产品

Primary Antibodies

AB218431

Anti-Androgen Receptor antibody [AR441]

Lab Essentials

  • 5
  • 2
Primary Antibodies

AB45089

Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2]

BOND RX™ Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (phospho S210 + S213) antibody [156C135.2] (AB45089)

  • 5
  • 3
Primary Antibodies

AB19066

Anti-Androgen Receptor antibody

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody (AB19066)

  • 0
  • 0
Primary Antibodies

AB226171

Anti-Androgen Receptor antibody

Lab Essentials

Immunoprecipitation - Anti-Androgen Receptor antibody (AB226171)

  • 0
  • 0
Primary Antibodies

AB9474

Anti-Androgen Receptor antibody [AR 441]

Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [AR 441] (AB9474)

  • 4
  • 12
Primary Antibodies

AB105225

Anti-Androgen Receptor antibody [SP107] - N-terminal

RabMAb|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - N-terminal (AB105225)

  • 4
  • 8
Primary Antibodies

AB202432

Alexa Fluor® 647 Anti-Androgen Receptor antibody [ER179(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Androgen Receptor antibody [ER179(2)] (AB202432)

  • 0
  • 0
Primary Antibodies

AB202690

Alexa Fluor® 488 Anti-Androgen Receptor antibody [ER179(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Androgen Receptor antibody [ER179(2)] (AB202690)

  • 0
  • 0
Primary Antibodies

AB108341

Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|Advanced Validation

Immunohistochemistry - Anti-Androgen Receptor antibody [ER179(2)] - ChIP Grade (AB108341)

  • 5
  • 4
Primary Antibodies

AB52615

Anti-Androgen Receptor antibody [EP670Y]

RabMAb|Recombinant|Lab Essentials

Western blot - Anti-Androgen Receptor antibody [EP670Y] (AB52615)

  • 4
  • 5
Primary Antibodies

AB206358

Alexa Fluor® 594 Anti-Androgen Receptor antibody [ER179(2)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Androgen Receptor antibody [ER179(2)] (AB206358)

  • 0
  • 0
Primary Antibodies

AB208088

Anti-Androgen Receptor antibody [EP670Y] - BSA and Azide free

RabMAb|Recombinant

Western blot - Anti-Androgen Receptor antibody [EP670Y] - BSA and Azide free (AB208088)

  • 0
  • 0
Primary Antibodies

AB227678

Anti-Androgen Receptor antibody [SP242] - C-terminal

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP242] - C-terminal (AB227678)

  • 5
  • 2
Primary Antibodies

AB236225

Anti-Androgen Receptor antibody [SP107] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP107] - BSA and Azide free (AB236225)

  • 0
  • 0
Primary Antibodies

AB236237

Anti-Androgen Receptor antibody [SP242] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor antibody [SP242] - BSA and Azide free (AB236237)

  • 0
  • 0

搭配使用产品

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88

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