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使用敲除细胞株进行验证重组RabMAb

重组Anti-AMPK alpha 1抗体[Y365] (ab32047)

  • Datasheet
  • SDS
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Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Flow Cytometry (Intracellular) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Immunoprecipitation - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Flow Cytometry (Intracellular) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
  • Anti-AMPK alpha 1 antibody [Y365] (ab32047)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y365] to AMPK alpha 1
  • Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase APC Carrier Free HRP PE

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概述

  • 产品名称

    Anti-AMPK alpha 1抗体[Y365]
    参阅全部 AMPK alpha 1 一抗
  • 描述

    兔单克隆抗体[Y365] to AMPK alpha 1
  • 宿主

    Rabbit
  • 特异性

    This antibody is specific for human AMPK alpha 1. This antibody shows low sensitivity in mouse and rat samples.

  • 经测试应用

    适用于: ICC/IF, Flow Cyt (Intra), WB, IHC-P, IPmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: African green monkey
  • 免疫原

    Synthetic peptide within Human AMPK alpha 1 aa 500 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q13131

  • 阳性对照

    • WB: HeLa, HepG2, C6, NIH/3T3 and MCF-7 cell lysate. Mouse liver, brain, retina, and skeletal muscle tissue lysates, RAW 264.7 cell lysate, Neuro2A cell lysate. IHC-P: Human cervical carcinoma and lung carcinoma tissues. ICC/IF: MCF-7 cells. Flow Cyt (intra): HeLa cells. IP: HeLa whole cell lysate (ab150035).
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    Y365
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

相关产品

  • Alternative Versions

    • Anti-AMPK alpha 1 antibody [Y365] - BSA and Azide free (ab210714)
    • Alexa Fluor® 568 Anti-AMPK alpha 1 antibody [Y365] (ab312428)
    • Alexa Fluor® 750 Anti-AMPK alpha 1 antibody [Y365] (ab321559)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • Hep G2 whole cell lysate (ab166833)
    • MCF7 whole cell lysate (ab29537)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human AMPK alpha 1 protein (ab202156)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10 - 245 kDa) (ab116028)
    • VeriBlot for IP Detection Reagent (HRP) (ab131366)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab32047于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/250.
Flow Cyt (Intra)
1/100 - 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB (5)
1/1000 - 1/5000. Predicted molecular weight: 63 kDa.

This antibody shows low sensitivity in mouse and rat samples.

IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
Mouse and rat species are recommended by WB application. 

IP
1/40 - 1/50.
说明
ICC/IF
1/250.
Flow Cyt (Intra)
1/100 - 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/5000. Predicted molecular weight: 63 kDa.

This antibody shows low sensitivity in mouse and rat samples.

IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
Mouse and rat species are recommended by WB application. 

IP
1/40 - 1/50.

靶标

  • 功能

    Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
  • 序列相似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession Q13131 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5562 Human
    • Entrez Gene: 105787 Mouse
    • Entrez Gene: 65248 Rat
    • Omim: 602739 Human
    • SwissProt: Q13131 Human
    • SwissProt: Q5EG47 Mouse
    • SwissProt: P54645 Rat
    • Unigene: 43322 Human
    • Unigene: 207004 Mouse
    • Unigene: 87789 Rat
    see all
  • 别名

    • 5 AMP activated protein kinase alpha 1catalytic subunit antibody
    • 5 AMP activated protein kinase catalytic alpha 1 chain antibody
    • 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
    • 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
    • AAPK1 antibody
    • AAPK1_HUMAN antibody
    • ACACA kinase antibody
    • acetyl CoA carboxylase kinase antibody
    • AI194361 antibody
    • AI450832 antibody
    • AL024255 antibody
    • AMP -activate kinase alpha 1 subunit antibody
    • AMP-activate kinase alpha 1 subunit antibody
    • AMP-activated protein kinase, catalytic, alpha -1 antibody
    • AMPK 1 antibody
    • AMPK alpha 1 antibody
    • AMPK alpha 1 chain antibody
    • AMPK antibody
    • AMPK subunit alpha 1 antibody
    • AMPK subunit alpha-1 antibody
    • AMPK1 antibody
    • AMPKa1 antibody
    • AMPKalpha1 antibody
    • C130083N04Rik antibody
    • cb116 antibody
    • EC 2.7.11.1 antibody
    • HMG CoA reductase kinase antibody
    • HMGCR kinase antibody
    • hormone sensitive lipase kinase antibody
    • Hydroxymethylglutaryl CoA reductase kinase antibody
    • im:7154392 antibody
    • kinase AMPK alpha1 antibody
    • MGC33776 antibody
    • MGC57364 antibody
    • OTTHUMP00000161795 antibody
    • OTTHUMP00000161796 antibody
    • PRKAA 1 antibody
    • PRKAA1 antibody
    • Protein kinase AMP activated alpha 1 catalytic subunit antibody
    • SNF1-like protein AMPK antibody
    • SNF1A antibody
    • Tau protein kinase PRKAA1 antibody
    • wu:fa94c10 antibody
    see all

图片

  • Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    All lanes : Anti-AMPK alpha 1 antibody [Y365] (ab32047) at 1/1000 dilution

    Lane 1 : Wild-type RAW 264.7 cell lysate
    Lane 2 : PRKAA1 knockout RAW 264.7 cell lysate
    Lane 3 : Mouse Liver cell lysate
    Lane 4 : Neuro2A cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 63 kDa
    Observed band size: 64 kDa why is the actual band size different from the predicted?



    False colour image of Western blot: Anti-AMPK alpha 1 antibody [Y365] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab32047 was shown to bind specifically to AMPK alpha 1. A band was observed at 64 kDa in wild-type RAW 264.7 cell lysates with no signal observed at this size in PRKAA1 knockout cell line ab280055 (knockout cell lysate ab280114). To generate this image, wild-type and PRKAA1 knockout RAW 264.7 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

  • Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: AMPK alpha knockout HAP1 cell lysate (20 µg)
    Lane 3: MCF7 cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab32047 observed at 63 kDa. Red - loading control, ab8245, observed at 37 kDa.


    ab32047 was shown to specifically react with AMPK alpha in wild-type HAP1 cells. No band was observed when AMPK alpha knockout samples were examined. Wild-type and AMPK alpha knockout samples were subjected to SDS-PAGE. ab32047 and ab8245 (loading control to GAPDH) were diluted 1/5000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling AMPK alpha 1 with purified ab32047 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9 before commencing with IHC staining protocol. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. 

  • Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Immunocytochemistry/ Immunofluorescence - Anti-AMPK alpha 1 antibody [Y365] (ab32047)

    Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling AMPK alpha 1 with purified ab32047 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Flow Cytometry (Intracellular) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Flow Cytometry (Intracellular) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)

    Intracellular Flow Cytometry analysis of HeLa cells labelling AMPK alpha 1 with purified ab32047 at 1/150 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

     

     

  • Immunoprecipitation - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Immunoprecipitation - Anti-AMPK alpha 1 antibody [Y365] (ab32047)

    ab32047 (purified) at 1/40 immunoprecipitating AMPK alpha 1 in HeLa whole cell lysate.

    Lane 1 (input): HeLa whole cell lysate (10µg)

    Lane 2 (+): ab32047 + HeLa whole cell lysate (10µg).

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab32047 in HeLa whole cell lysate.

    For western blotting, ab131366 VeriBlot for IP Detection Reagent (HRP) was used for detection (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    All lanes : Anti-AMPK alpha 1 antibody [Y365] (ab32047) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
    Lane 3 : Mouse liver tissue lysate
    Lane 4 : Mouse brain tissue lysate
    Lane 5 : Mouse kidney tissue lysate
    Lane 6 : Mouse retina tissue lysate
    Lane 7 : Mouse skeletal muscle tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 63 kDa
    Observed band size: 63 kDa
    Additional bands at: 40 kDa (possible non-specific binding)


    Exposure time: 3 minutes


    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    This antibody shows low sensitivity in mouse samples.

  • Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    All lanes : Anti-AMPK alpha 1 antibody [Y365] (ab32047) at 1/20000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate
    Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 4 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
    Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate
    Lane 6 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate
    Lane 7 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 63 kDa
    Observed band size: 63 kDa


    Exposure time: 180 seconds


    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This antibody shows low sensitivity in mouse and rat samples.

  • Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Western blot - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    All lanes : Anti-AMPK alpha 1 antibody [Y365] (ab32047) at 1/2000 dilution (purified)

    Lane 1 : C6 cell lysate
    Lane 2 : NIH/3T3 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 63 kDa
    Observed band size: 63 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.
    This antibody shows low sensitivity in mouse and rat samples.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analaysis of human cervical carcinoma tissue labelling AMPK alpha 1 with unpurified ab32047 at a dilution of 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Flow Cytometry (Intracellular) - Anti-AMPK alpha 1 antibody [Y365] (ab32047)

    Overlay histogram showing HeLa cells stained with unpurified ab32047 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab32047, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. 

     

     

  • Anti-AMPK alpha 1 antibody [Y365] (ab32047)
    Anti-AMPK alpha 1 antibody [Y365] (ab32047)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (192)

发表研究结果有使用 ab32047?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab32047 被引用在 192 文献中.

  • Ji P  et al. Genetically engineered probiotics as catalytic glucose depriver for tumor starvation therapy. Mater Today Bio 18:100515 (2023). PubMed: 36582449
  • Wang YL  et al. Sestrin2 mediates FOXM1 expression to block the EMT process in non-small cell lung cancer through the AMPK/YAP pathway. Neoplasma 70:46-57 (2023). PubMed: 36620877
  • Huang S  et al. Canagliflozin ameliorates the development of NAFLD by preventing NLRP3-mediated pyroptosis through FGF21-ERK1/2 pathway. Hepatol Commun 7:e0045 (2023). PubMed: 36757426
  • Wang Z  et al. FOXA2 plays a critical role in hepatocellular carcinoma progression and lenvatinib-associated drug resistance. Biosci Trends 17:136-147 (2023). PubMed: 36823043
  • Zhang J  et al. Effect of ketogenic diet on exercise tolerance and transcriptome of gastrocnemius in mice. Open Life Sci 18:20220570 (2023). PubMed: 36852401
View all Publications for this product

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1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-AMPK alpha 1 antibody [Y365]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Gastric Cancer Cell Line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
10 µg
Specification
Gastric Cancer Cell Line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 13 2020

Western blot abreview for Anti-AMPK alpha 1 antibody [Y365]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse macrophage cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
3 µg
Specification
Mouse macrophage cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 02 2020

Western blot abreview for Anti-AMPK alpha 1 antibody [Y365]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (Muscle fibers)
Gel Running Conditions
Reduced Denaturing (8% acrylamide)
Loading amount
50 µg
Specification
Muscle fibers
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Irene Tobias

Verified customer

提交于 Mar 21 2018

Immunohistochemistry free floating abreview for Anti-AMPK alpha 1 antibody [Y365]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain slide)
Specification
Brain slide
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Delphine Franssen

Verified customer

提交于 May 17 2017

Western blot abreview for Anti-AMPK alpha 1 antibody [Y365]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Bos taurus Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (4-15% Gradient)
Loading amount
20 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Julie Kim

Verified customer

提交于 May 05 2017

Western blot abreview for Anti-AMPK alpha 1 antibody [Y365]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (human HepG2 cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
human HepG2 cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Dec 05 2016

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