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Anti-AMPK alpha 1抗体(ab3759)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (30)

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Western blot - Anti-AMPK alpha 1 antibody (ab3759)
  • Western blot - Anti-AMPK alpha 1 antibody (ab3759)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody (ab3759)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody (ab3759)
  • Immunoprecipitation - Anti-AMPK alpha 1 antibody (ab3759)

Key features and details

  • Rabbit polyclonal to AMPK alpha 1
  • Suitable for: IP, WB, IHC-P
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-AMPK alpha 1抗体
    参阅全部 AMPK alpha 1 一抗
  • 描述

    兔多克隆抗体to AMPK alpha 1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IP, WB, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat, Cow, Human
    预测可用于: Orangutan
  • 免疫原

    Synthetic peptide within Human AMPK alpha 1 aa 350-450. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. BAA36547.1 (GeneID 5562).
    Database link: Q13131

  • 阳性对照

    • WB: Bovine aortic endothelial cell extracts. Rat aortic smooth muscle cell extracts. HepG2 extracts. Human aortic endothelial cell extract. HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IHC-P: Human muscle and pancreas tissue. IP: AMPK alpha 1 IP in HeLa cell lysate.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Neurodegenerative disease
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human AMPK alpha 1 protein (ab202156)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3759于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP
Use at 2-5 µg/mg of lysate.
WB (1)
1/500 - 1/10000. Predicted molecular weight: 63 kDa.
IHC-P
Use at an assay dependent concentration.
说明
IP
Use at 2-5 µg/mg of lysate.
WB
1/500 - 1/10000. Predicted molecular weight: 63 kDa.
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
  • 序列相似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
    Contains 1 protein kinase domain.
  • Target information above from: UniProt accession Q13131 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5562 Human
    • Entrez Gene: 105787 Mouse
    • Entrez Gene: 65248 Rat
    • Omim: 602739 Human
    • SwissProt: Q13131 Human
    • SwissProt: Q5EG47 Mouse
    • SwissProt: P54645 Rat
    • Unigene: 43322 Human
    • Unigene: 207004 Mouse
    • Unigene: 87789 Rat
    see all
  • 别名

    • 5 AMP activated protein kinase alpha 1catalytic subunit antibody
    • 5 AMP activated protein kinase catalytic alpha 1 chain antibody
    • 5' AMP activated protein kinase catalytic subunit alpha 1 antibody
    • 5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
    • AAPK1 antibody
    • AAPK1_HUMAN antibody
    • ACACA kinase antibody
    • acetyl CoA carboxylase kinase antibody
    • AI194361 antibody
    • AI450832 antibody
    • AL024255 antibody
    • AMP -activate kinase alpha 1 subunit antibody
    • AMP-activate kinase alpha 1 subunit antibody
    • AMP-activated protein kinase, catalytic, alpha -1 antibody
    • AMPK 1 antibody
    • AMPK alpha 1 antibody
    • AMPK alpha 1 chain antibody
    • AMPK antibody
    • AMPK subunit alpha 1 antibody
    • AMPK subunit alpha-1 antibody
    • AMPK1 antibody
    • AMPKa1 antibody
    • AMPKalpha1 antibody
    • C130083N04Rik antibody
    • cb116 antibody
    • EC 2.7.11.1 antibody
    • HMG CoA reductase kinase antibody
    • HMGCR kinase antibody
    • hormone sensitive lipase kinase antibody
    • Hydroxymethylglutaryl CoA reductase kinase antibody
    • im:7154392 antibody
    • kinase AMPK alpha1 antibody
    • MGC33776 antibody
    • MGC57364 antibody
    • OTTHUMP00000161795 antibody
    • OTTHUMP00000161796 antibody
    • PRKAA 1 antibody
    • PRKAA1 antibody
    • Protein kinase AMP activated alpha 1 catalytic subunit antibody
    • SNF1-like protein AMPK antibody
    • SNF1A antibody
    • Tau protein kinase PRKAA1 antibody
    • wu:fa94c10 antibody
    see all

图片

  • Western blot - Anti-AMPK alpha 1 antibody (ab3759)
    Western blot - Anti-AMPK alpha 1 antibody (ab3759)
    All lanes : Anti-AMPK alpha 1 antibody (ab3759) at 0.1 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 63 kDa


    Exposure time: 3 seconds


    Lysated prepared using NETN lysis buffer.

  • Western blot - Anti-AMPK alpha 1 antibody (ab3759)
    Western blot - Anti-AMPK alpha 1 antibody (ab3759)

    Samples: Extracts from

    1. Bovine aortic endothelial cells

    2. Rat aortic smooth muscle cells

    3. HepG2 cells

    4. Human aortic endothelial cells

    Antibody: Affinity purified Rabbit anti-AMPK alpha 1 (ab3759) used at 2ug/ml (1/500).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody (ab3759)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody (ab3759)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling AMPK alpha 1 with ab3759 at 1/5000 (0.2µg/ml). Detection: DAB.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody (ab3759)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 1 antibody (ab3759)

    IHC image of AMPK alpha 1 staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3759, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunoprecipitation - Anti-AMPK alpha 1 antibody (ab3759)
    Immunoprecipitation - Anti-AMPK alpha 1 antibody (ab3759)

    Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.

    Lane 1 and 2: ab3759 used for IP at 3 µg per reaction, two lots.

    Lane 3: Control IgG.

    For blotting immunoprecipitated AMPK alpha 1, ab3759 was used at 0.4 µg/ml.

    Detection: Chemiluminescence with an exposure time of 3 seconds.

实验方案

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (30)

发表研究结果有使用 ab3759?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab3759 被引用在 30 文献中.

  • Li T  et al. MicroRNA-206 inhibition and activation of the AMPK/Nampt signalling pathway enhance sevoflurane post-conditioning-induced amelioration of myocardial ischaemia/reperfusion injury. J Drug Target 28:80-91 (2020). PubMed: 31092059
  • Fan F  et al. Regulation of PGC-1a mediated by acetylation and phosphorylation in MPP+ induced cell model of Parkinson's disease. Aging (Albany NY) 12:9461-9474 (2020). PubMed: 32452827
  • Bai R  et al. Apple pomace and rosemary extract ameliorates hepatic steatosis in fructose-fed rats: Association with enhancing fatty acid oxidation and suppressing inflammation. Exp Ther Med 20:1975-1986 (2020). PubMed: 32782507
  • Ono H  et al. AMPK Complex Activation Promotes Sarcolemmal Repair in Dysferlinopathy. Mol Ther 28:1133-1153 (2020). PubMed: 32087766
  • Zimmermann HR  et al. Brain-specific repression of AMPKa1 alleviates pathophysiology in Alzheimer's model mice. J Clin Invest 130:3511-3527 (2020). PubMed: 32213711
View all Publications for this product

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-AMPK alpha 1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Human HEK293T cell line)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
25 µg
Specification
Human HEK293T cell line
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Vladimir Milenkovic

Verified customer

提交于 Apr 07 2022

Question

Inquiry: Dear Abcam, My question is about the specificity of AMPK alpha1 antibody (2B7, catalog no: ab110036). Is there any evidence (ie. Western) whether this antibody does or does not cross-react with AMPK alpha2? Thank you.

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Abcam community

Verified customer

Asked on Apr 24 2012

Answer

Thank you for contacting us.

Unfortunately, we have not tested this antibody for cross reactivity with AMPK alpha 2 and are unsure whether it will cross react with this protein as we do not have any data for support.

We do offer two other products against AMPK alpha 2 which may satisfy your requirement however. According to the information available to us and on the datasheet, Ab3759 does not react withAMPK alpha 2 (https://www.abcam.com/AMPK-alpha-1-antibody-ab3759.html).

Ab31958 has not been tested for cross reactivity however a BLAST of the immunogen sequence did not reveal appropriate homology with AMPK alpha 1 to suggest cross reactivity (https://www.abcam.com/AMPK-alpha-1-antibody-ab31958.html).


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

回复于 Apr 24 2012

Question

Ab3759 - Rabbit polyclonal to AMPK alpha 1 - does this antibody cross-react with alpha 2? Ab3760 - Rabbit polyclonal to AMPK alpha 2 - does this antibody cross-react with alpha 1?

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Abcam community

Verified customer

Asked on Sep 14 2005

Answer

Thank you for your enquiry. Our source for ab3759 and ab3760 has told me that the antibodies do not cross-react with the homologs. If you have any additional questions, please contact us again.

Read More

Abcam Scientific Support

回复于 Sep 14 2005

Question

can the anti-AMPK alpha1 (ab3759) be used in immunohistochemistry?

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Abcam community

Verified customer

Asked on Dec 10 2003

Answer

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

Read More

Abcam Scientific Support

回复于 Dec 10 2003

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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