AB234750

Anti-Amphiregulin 抗体

Name: Amphiregulin抗体 (ab234750)| Abcam中文官网
Brand: Abcam Limited
SKU: AB234750
Price: 4890 CNY
Availability: InStock

Anti-Amphiregulin antibody

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(5 Publications)

Rabbit Polyclonal Amphiregulin antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human AREG aa 100-150.

查看别名

AREGB, SDGF, AREG, Amphiregulin, AR, Colorectum cell-derived growth factor, CRDGF

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiregulin antibody (AB234750)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiregulin antibody (AB234750)

Paraffin-embedded human breast cancer tissue stained for Amphiregulin using ab234750 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Amphiregulin antibody (AB234750)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Amphiregulin antibody (AB234750)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Amphiregulin (green) using ab234750 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® congugated Goat Anti-Rabbit IgG (H+L).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiregulin antibody (AB234750)

Paraffin-embedded human colon cancer tissue stained for Amphiregulin using ab234750 at 1/100 dilution in immunohistochemical analysis.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

ICC/IF, IHC-P

applications

免疫原

Recombinant Fragment Protein within Human AREG aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P15514

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

纯化说明

Purity >95%.

存储溶液

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

'Amphiregulin' also known as 'AREG' or 'Amphiregulin protein' is a member of the epidermal growth factor (EGF) family. It is a 252 amino acid protein with a predicted mass of approximately 27-29 kDa. Amphiregulin shows expression in various tissues including epithelial cells fibroblasts and immune cells. This protein functions as a ligand for the EGF receptor (EGFR) leading to receptor dimerization and subsequent activation of downstream signaling pathways.

Biological function summary

'Amphiregulin' plays a critical role in cell proliferation differentiation and tissue remodeling. It is not part of a constitutive complex but rather exerts its effects through interaction with EGFR a receptor tyrosine kinase. Amphiregulin contributes significantly to mammary gland development and has a notable role in wound healing. Beyond its developmental roles Amphiregulin can impact immune modulation and inflammation processes.

Pathways

'Amphiregulin' significantly contributes to the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways. These pathways influence cell survival growth and proliferation. Amphiregulin interacts with EGFR and by extension has relationships with other EGFR ligands such as EGF and transforming growth factor-alpha (TGF-alpha). These pathways underline the importance of Amphiregulin in maintaining cellular functions and responding to external stimuli.

'Amphiregulin' has strong associations with cancer and asthma. Overexpression of Amphiregulin can lead to increased cancer cell proliferation and it has been implicated in diverse cancers such as breast and colorectal cancer. Additionally the protein plays a role in airway remodeling in asthma contributing to bronchial hyper-responsiveness. In these contexts Amphiregulin's interactions with EGFR are central influencing disease progression and response to treatment.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

See full target information AREG

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2412233 PubMed39680752

2024

SREBP1c-Mediated Transcriptional Repression of YME1L1 Contributes to Acute Kidney Injury by Inducing Mitochondrial Dysfunction in Tubular Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wang Xin,Jie Zhou,Yuzhu Peng,Shuiqin Gong,Wenhao Liao,Yaqin Wang,Xixin Huang,Yang Mao,Mengying Yao,Shaozong Qin,Jiachuan Xiong,Yan Li,Qigang Lan,Yinghui Huang,Jinghong Zhao

Cellular & molecular immunology 22:97-110 PubMed39653767

2024

Esophageal ILC2s mediate abnormal epithelial remodeling in eosinophilic esophagitis via Areg-EGFR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

MinYeong Lim,Taesoo Kim,Hyesung Kim,Bo Gun Jang,Jae Kyung Myung,Hye Young Kim

Investigative ophthalmology & visual science 65:43 PubMed39330987

2024

Single-Cell Transcriptomic Analysis Reveals Dynamic Cellular Processes in Corneal Epithelium During Wound Healing in Cynomolgus Monkeys.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Zhou,Zhuo-Xing Shi,Zhong Liu,Shu-Rui Ke,Chao-Yang Wang,Xiao-Lin Liang,Qiu-Ling Hu,Qi-Kai Zhang,Dong-Liang Wang,Li Sun,Yu-Heng Lin,Qi Dai,Ying-Feng Zheng

Clinical and translational medicine 13:e1166 PubMed36639835

2023

XBP1-elicited environment by chemotherapy potentiates repopulation of tongue cancer cells by enhancing miR-22/lncRNA/KAT6B-dependent NF-κB signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoting Jia,Ge Wang,Lihong Wu,Hao Pan,Li Ling,Jianlei Zhang,Qingquan Wen,Jie Cui,Zhimin He,Bin Qi,Shuxu Zhang,Liyun Luo,Guopei Zheng

Journal of ovarian research 15:129 PubMed36476625

2022

Luteinizing hormone stimulates the expression of amphiregulin in human theca cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Yiping Zhong,Xiaoting Shen,Xi Guo,Rihan Wu,Turui Yang,Minghui Chen

View all publications

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Anti-Amphiregulin 抗体

Anti-Amphiregulin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiregulin antibody (AB234750)

Immunocytochemistry/ Immunofluorescence - Anti-Amphiregulin antibody (AB234750)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiregulin antibody (AB234750)