AB180722

Anti-Amphiregulin抗体

Name: Amphiregulin抗体 (ab180722)| Abcam中文官网
Brand: Abcam Limited
SKU: AB180722
Price: 4606 CNY
Availability: InStock
Rating: 4 (3 reviews)

Anti-Amphiregulin antibody

(3 Reviews)

(4 Publications)

Rabbit Polyclonal Amphiregulin antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human AREG.

查看别名

AREGB, SDGF, AREG, Amphiregulin, AR, Colorectum cell-derived growth factor, CRDGF

3 Images

Supplier Data

Western blot - Anti-Amphiregulin antibody (AB180722)

Blocking buffer : 3% nonfat dry milk in TBST. PMID : 9642297.

All lanes:

Western blot - Anti-Amphiregulin antibody (ab180722) at 1/1000 dilution

All lanes:

A549 (Human lung carcinoma cell line) whole cell lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 28 kDa

Observed band size: 43 kDa

false

Exposure time: 3s

Supplier Data

Western blot - Anti-Amphiregulin antibody (AB180722)

Blocking buffer : 3% nonfat dry milk in TBST. PMID : 9642297.

All lanes:

Western blot - Anti-Amphiregulin antibody (ab180722) at 1/1000 dilution

All lanes:

Mouse brain lysate at 25 µg

Secondary

All lanes:

HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Predicted band size: 28 kDa

Observed band size: 43 kDa

false

Exposure time: 300s

Supplier Data

Western blot - Anti-Amphiregulin antibody (AB180722)

All lanes:

Western blot - Anti-Amphiregulin antibody (ab180722) at 1/500 dilution

Lane 1:

MCF7 cell extracts

Lane 2:

A549 cell extracts

Lane 3:

HT29 cell extracts

Predicted band size: 28 kDa

false

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Human

应用

applications

免疫原

Recombinant Full Length Protein corresponding to Human AREG.

P15514

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

'Amphiregulin' also known as 'AREG' or 'Amphiregulin protein' is a member of the epidermal growth factor (EGF) family. It is a 252 amino acid protein with a predicted mass of approximately 27-29 kDa. Amphiregulin shows expression in various tissues including epithelial cells fibroblasts and immune cells. This protein functions as a ligand for the EGF receptor (EGFR) leading to receptor dimerization and subsequent activation of downstream signaling pathways.

Biological function summary

'Amphiregulin' plays a critical role in cell proliferation differentiation and tissue remodeling. It is not part of a constitutive complex but rather exerts its effects through interaction with EGFR a receptor tyrosine kinase. Amphiregulin contributes significantly to mammary gland development and has a notable role in wound healing. Beyond its developmental roles Amphiregulin can impact immune modulation and inflammation processes.

Pathways

'Amphiregulin' significantly contributes to the mitogen-activated protein kinase (MAPK) and phosphoinositide 3-kinase (PI3K) pathways. These pathways influence cell survival growth and proliferation. Amphiregulin interacts with EGFR and by extension has relationships with other EGFR ligands such as EGF and transforming growth factor-alpha (TGF-alpha). These pathways underline the importance of Amphiregulin in maintaining cellular functions and responding to external stimuli.

'Amphiregulin' has strong associations with cancer and asthma. Overexpression of Amphiregulin can lead to increased cancer cell proliferation and it has been implicated in diverse cancers such as breast and colorectal cancer. Additionally the protein plays a role in airway remodeling in asthma contributing to bronchial hyper-responsiveness. In these contexts Amphiregulin's interactions with EGFR are central influencing disease progression and response to treatment.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.

See full target information AREG

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 19:370 PubMed34789290

2021

Nickel nanoparticle-induced cell transformation: involvement of DNA damage and DNA repair defect through HIF-1α/miR-210/Rad52 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqun Mo,Yue Zhang,Yuanbao Zhang,Jiali Yuan,Luke Mo,Qunwei Zhang

iScience 24:103243 PubMed34746706

2021

Renal subcapsular delivery of PGE promotes kidney repair by activating endogenous Sox9 stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shang Chen,Haoyan Huang,Yue Liu,Chen Wang,Xiaoniao Chen,Yuqiao Chang,Yuhao Li,Zhikun Guo,Zhibo Han,Zhong-Chao Han,Qiang Zhao,Xiang-Mei Chen,Zongjin Li

Molecular medicine reports 24: PubMed34396425

2021

HOXB13 suppresses proliferation, migration and invasion, and promotes apoptosis of gastric cancer cells through transcriptional activation of VGLL4 to inhibit the involvement of TEAD4 in the Hippo signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Geng,Guangli Liu,Jiandong Hu,Jianchao Li,Dong Wang,Shenshan Zou,Xintao Xu

Frontiers in oncology 10:160 PubMed32154166

2020

Long Non-coding RNA MIR4435-2HG Promotes Colorectal Cancer Proliferation and Metastasis Through miR-206/YAP1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xinhua Dong,Zhen Yang,Hongwei Yang,Dongyan Li,Xinguang Qiu

View all publications

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Anti-Amphiregulin抗体

Anti-Amphiregulin antibody

Western blot - Anti-Amphiregulin antibody (AB180722)

Western blot - Anti-Amphiregulin antibody (AB180722)

Western blot - Anti-Amphiregulin antibody (AB180722)