AB76124

Anti-Amphiphysin抗体[EP2061Y]

Anti-Amphiphysin antibody [EP2061Y]

BOND RX™ Validated
RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal Amphiphysin antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

查看别名

AMPH1, AMPH, Amphiphysin

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiphysin antibody [EP2061Y] (AB76124)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiphysin antibody [EP2061Y] (AB76124)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue with ab76124 at 1/5000 (0.23 ug/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

The section was incubated with ab76124 for 30 mins at room temperature.

Counterstained with hematoxylin. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

Lab

Western blot - Anti-Amphiphysin antibody [EP2061Y] (AB76124)

Exposure time : Lane 1, 20 seconds; Lane 2, 10 seconds; Lane 3, 5 seconds.

All lanes:

Western blot - Anti-Amphiphysin antibody [EP2061Y] (ab76124) at 1/100000 dilution

Lane 1:

Human brain lysate at 15 µg

Lane 2:

Mouse brain lysate at 15 µg

Lane 3:

Rat brain lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 128 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-Amphiphysin antibody [EP2061Y] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EP2061Y

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100000", "WB-species-notes": "" } } }

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产品详情

The production method for this product has been changed from hybridoma to recombniant on 16th April 2024

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Amphiphysin also known as BIN1 or PHIPBP1 functions mechanically as a protein implicated in endocytosis. It has a molecular mass around 128 kDa. You find it expressed in various tissues but most notably in the brain and muscle where it plays essential roles in synaptic vesicle recycling. This protein has a BAR domain that allows it to bind to lipid membranes facilitating membrane curvature necessary for vesicle budding in neurons.

Biological function summary

In many cellular contexts Amphiphysin acts in forming a heterodimer complex with Dynamin which is necessary for synaptic vesicle endocytosis. The protein serves a role in membrane dynamics and is involved in the scission of newly formed vesicles from the plasma membrane. Using Amphiphysin antibodies can help detect or isolate this protein aiding in the study of synaptic transmission and neuromuscular functions.

Pathways

The protein plays a role in clathrin-mediated endocytosis and interacts closely with the protein Clathrin. Amphiphysin antibodies help in studying these relationships as Amphiphysin modulates actin cytoskeleton organization. It also relates to the Dynamin family through its function in synaptic vesicle retrieval highlighting its central role within the synaptic function pathway.

Mutations or dysregulation of Amphiphysin relate to conditions like Stiff Person Syndrome and Barre-Liéou syndrome. These are neurological disorders where the malfunction of Amphiphysin adversely affects synaptic function and it may interact with GABAergic pathways. Anti-amphiphysin antibodies can be used in diagnostics to identify autoimmune response in these disorders showing the protein's significance in maintaining proper neurological health.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.

See full target information AMPH

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 11:404 PubMed40854914

2025

Multi-omics analysis reveals RNA polymerase II degradation as a novel mechanism of PF-3758309's anti-tumor activity.

Applications

Unspecified application

Species

Unspecified reactive species

Xinglong Jia,Jingdan Zhang,Lulu Pan,Jingliang He,Mingrui Zhu,Lei Zhao,Xingyu Zhang,Wensi Zhao,Dong Xie,Xiaoyan Shen,Bin Liu,Minjia Tan

View all publications

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Anti-Amphiphysin抗体[EP2061Y]