重组Anti-Amphiphysin抗体[EP2060Y] (ab52646)
![Western blot - Anti-Amphiphysin antibody [EP2060Y] (ab52646)](https://www.abcam.cn/ps/products/52/ab52646/Images/ab52646-65072-anti-amphiphysin-antibody-ep2060y-western-blot.jpg "Western blot - Anti-Amphiphysin antibody [EP2060Y] (ab52646)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2060Y] to Amphiphysin
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Amphiphysin抗体[EP2060Y]
参阅全部 Amphiphysin 一抗 -
描述
兔单克隆抗体[EP2060Y] to Amphiphysin -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human brain lysate. IHC-P: Human brain tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP2060Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab52646于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/25000 - 1/50000. Detects a band of approximately 128 kDa (predicted molecular weight: 76 kDa).
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 说明 |
|---|
|
WB
1/25000 - 1/50000. Detects a band of approximately 128 kDa (predicted molecular weight: 76 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton. -
组织特异性
Neurons, certain endocrine cell types and spermatocytes. -
序列相似性
Contains 1 BAR domain.
Contains 1 SH3 domain. -
细胞定位
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 273 Human
- Entrez Gene: 218038 Mouse
- Entrez Gene: 60668 Rat
- Omim: 600418 Human
- SwissProt: P49418 Human
- SwissProt: Q7TQF7 Mouse
- SwissProt: O08838 Rat
- Unigene: 592182 Human
see all -
别名
- Amp antibody
- AMPH 1 antibody
- AMPH antibody
see all
图片
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Western blot - Anti-Amphiphysin antibody [EP2060Y] (ab52646)Anti-Amphiphysin antibody [EP2060Y] (ab52646) at 1/100000 dilution + Human brain lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 76 kDa
Observed band size: 128 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiphysin antibody [EP2060Y] (ab52646)](https://www.abcam.cn/ps/products/52/ab52646/Images/ab52646-65071-anti-amphiphysin-antibody-ep2060y-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amphiphysin antibody [EP2060Y] (ab52646)Ab52646 (1/100) staining human Amphiphysin in human brain tissue by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Amphiphysin antibody [EP2060Y] (ab52646)](https://www.abcam.cn/ps/products/52/ab52646/Images/ab52646-5-benefits-of-recombinant-antibodies.png)
Anti-Amphiphysin antibody [EP2060Y] (ab52646)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab52646 被引用在 3 文献中.
- Buser DP et al. Quantitative proteomics reveals reduction of endocytic machinery components in gliomas. EBioMedicine 46:32-41 (2019). PubMed: 31331834
- Bosch PJ et al. Proteomics Analysis of Dorsal Striatum Reveals Changes in Synaptosomal Proteins following Methamphetamine Self-Administration in Rats. PLoS One 10:e0139829 (2015). WB . PubMed: 26484527
- Kumar GS et al. Quantitative proteomics for identifying biomarkers for tuberculous meningitis. Clin Proteomics 9:12 (2012). IHC . PubMed: 23198679