Anti-Amino-terminal enhancer of split/AES抗体(ab118881)
Key features and details
- Rabbit polyclonal to Amino-terminal enhancer of split/AES
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Amino-terminal enhancer of split/AES抗体
参阅全部 Amino-terminal enhancer of split/AES 一抗 -
描述
兔多克隆抗体to Amino-terminal enhancer of split/AES -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human Amino-terminal enhancer of split/AES (C terminal) conjugated to keyhole limpet haemocyanin.
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阳性对照
- IHC-P: Human skeletal muscle. WB: HEK-293 cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab118881于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa.
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| IHC-P |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 21 kDa. |
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IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Transcriptional corepressor. Acts as dominant repressor towards other family members. Inhibits NF-kappa-B-regulated gene expression. May be required for the initiation and maintenance of the differentiated state. Essential for the transcriptional repressor activity of SIX3 during retina and lens development. -
组织特异性
Found predominantly in muscle, heart and Placenta. In fetal tissues, abundantly expressed in the heart, lung, kidney, brain and liver. -
序列相似性
Belongs to the WD repeat Groucho/TLE family. -
结构域
Lacks the C-terminal WD repeats. -
翻译后修饰
Ubiquitinated by XIAP/BIRC4. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 166 Human
- Entrez Gene: 14797 Mouse
- Entrez Gene: 29466 Rat
- Omim: 600188 Human
- SwissProt: Q08117 Human
- SwissProt: P63002 Mouse
- SwissProt: P63003 Rat
- Unigene: 515053 Human
see all -
别名
- Aes 1 antibody
- Aes 2 antibody
- aes antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amino-terminal enhancer of split/AES antibody (ab118881)Human skeletal muscle (formalin fixed, paraffin embedded) stained with ab118881 at 2.5 µg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
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Western blot - Anti-Amino-terminal enhancer of split/AES antibody (ab118881)Lane 1 : Anti-Amino-terminal enhancer of split/AES antibody (ab118881) at 1 µg/ml
Lane 2 : Anti-Amino-terminal enhancer of split/AES antibody (ab118881) at 2 µg/ml
Lane 3 : Anti-Amino-terminal enhancer of split/AES antibody (ab118881) at 4 µg/ml
All lanes : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Predicted band size: 21 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab118881 尚未被引用在任何文献中。