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重组RabMAb

重组Anti-AMH抗体[EPR23380-171] (ab272221)

Submit a review Submit a question References (1)
Immunohistochemistry (Frozen sections) - Anti-AMH antibody [EPR23380-171] (ab272221)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR23380-171] (ab272221)
  • Immunohistochemistry (Frozen sections) - Anti-AMH antibody [EPR23380-171] (ab272221)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR23380-171] (ab272221)
  • Anti-AMH antibody [EPR23380-171] (ab272221)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23380-171] to AMH
  • Suitable for: IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-AMH抗体[EPR23380-171]
    参阅全部 AMH 一抗

  • 描述

    兔单克隆抗体[EPR23380-171] to AMH

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-Fr, IHC-Pmore details
    不适用于: IP or WB

  • 种属反应性

    与反应: Mouse, Rat
    不与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Mouse ovary tissue; Rat ovary tissue. IHC-Fr: Mouse ovary tissue; Rat ovary tissue.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab272221于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr
1/100.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
说明
IHC-Fr
1/100.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
应用说明
Is unsuitable for IP or WB.

靶标

  • 相关性

    Anti mullerian hormone (AMH) is a member of the TGF beta superfamily. It is secreted as a homodimeric 140kD disulphide linked precursor that is cleaved to release the mature 30kD homodimer. Originally classified as a foetal testicular hormone that inhibits Mullerian duct development, AMH is expressed post natally by immature Sertoli cells, and to a lesser degree by granulosa cells. AMH plays a role in testicular differentiation and in the regulation of ovarian follicle growth.

  • 细胞定位

    Secreted

  • 数据库链接

    • 别名

      • Anti muellerian hormone antibody
      • MIF antibody
      • MIS antibody
      • Muellerian inhibiting factor antibody
      • Mullerian inhibiting substance antibody
      see all

    图片

    • Immunohistochemistry (Frozen sections) - Anti-AMH antibody [EPR23380-171] (ab272221)
      Immunohistochemistry (Frozen sections) - Anti-AMH antibody [EPR23380-171] (ab272221)

      4% PFA-fixed, 0.2% Triton X-100 permeabilised rat ovary tissue labeling AMH with ab272221 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Cytoplasmic staining on granulosa cells of follicle.The nuclear counter stain is DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR23380-171] (ab272221)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR23380-171] (ab272221)

      Immunohistochemical analysis of paraffin-embedded Mouse ovary tissue labeling AMH with ab272221 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulosa cells of mouse ovary. The section was incubated with ab272221 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Immunohistochemistry (Frozen sections) - Anti-AMH antibody [EPR23380-171] (ab272221)
      Immunohistochemistry (Frozen sections) - Anti-AMH antibody [EPR23380-171] (ab272221)

      4% PFA-fixed, 0.2% Triton X-100 permeabilised mouse ovary tissue labeling AMH with ab272221 at 1/100 dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Cytoplasmic staining on granulosa cells of follicle.The nuclear counter stain is DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR23380-171] (ab272221)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMH antibody [EPR23380-171] (ab272221)

      Immunohistochemical analysis of paraffin-embedded Rat ovary tissue labeling AMH with ab272221 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on granulosa cells of rat ovary. The section was incubated with ab272221 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    • Anti-AMH antibody [EPR23380-171] (ab272221)
      Anti-AMH antibody [EPR23380-171] (ab272221)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (1)

    发表研究结果有使用 ab272221？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab272221 被引用在 1 文献中.

    • Ye M  et al. Creating a semi-opened micro-cavity ovary through sacrificial microspheres as an in vitro model for discovering the potential effect of ovarian toxic agents. Bioact Mater 26:216-230 (2023). PubMed: 36936809

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