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AB167394

重组Anti-AMF抗体[EPR11663(B)]

Anti-AMF antibody [EPR11663(B)]

4

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal AMF antibody. Suitable for IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

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Glucose-6-phosphate isomerase, GPI, Autocrine motility factor, Neuroleukin, Phosphoglucose isomerase, Phosphohexose isomerase, Sperm antigen 36, AMF, NLK, PGI, PHI, SA-36

11 Images
Western blot - Anti-AMF antibody [EPR11663(B)] (AB167394)
  • WB

Lab

Western blot - Anti-AMF antibody [EPR11663(B)] (AB167394)

Lanes 1-4 : Merged signal (red and green). Green - ab167394 observed at 63 kDa. Red - loading control ab8245 observed at 36 kDa.

ab167394 Anti-AMF antibody [EPR11663(B)] was shown to specifically react with AMF in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266834 (knockout cell lysate ab257458) was used. Wild-type and AMF knockout samples were subjected to SDS-PAGE. ab167394 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-AMF antibody [EPR11663(B)] (ab167394) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

GPI knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human GPI (AMF) knockout HEK-293T cell line (<a href='/products/cell-lines/human-gpi-amf-knockout-hek-293t-cell-line-ab266834'>ab266834</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 63 kDa

Observed band size: 63 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-AMF antibody [EPR11663(B)] (AB167394)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AMF antibody [EPR11663(B)] (AB167394)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling AFM with purified ab167394 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (ab150077). Nuclei counterstained with DAPI (blue).

Control : PBS only

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (AB167394)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (AB167394)

ab167394 showing +ve staining in Human normal brain.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-AMF antibody [EPR11663(B)] (AB167394)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-AMF antibody [EPR11663(B)] (AB167394)

Intracellular flow cytometric analysis of permeabilized A549 cells, labeling AFM with ab167394 at 1/10 dilution.

Western blot - Anti-AMF antibody [EPR11663(B)] (AB167394)
  • WB

Unknown

Western blot - Anti-AMF antibody [EPR11663(B)] (AB167394)

All lanes:

Western blot - Anti-AMF antibody [EPR11663(B)] (ab167394) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

HT29 cell lysate at 10 µg

Lane 4:

A549 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 63 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-AMF antibody [EPR11663(B)] (AB167394)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-AMF antibody [EPR11663(B)] (AB167394)

Immunofluorescent analysis of HepG2 cells labeling AFM with ab167394 at 1/100 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (AB167394)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (AB167394)

ab167394 showing +ve staining in Human normal pancreas.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (AB167394)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (AB167394)

ab167394 showing +ve staining in Human ovarian carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (AB167394)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (AB167394)

ab167394 showing +ve staining in Human skeletal muscle.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (AB167394)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (AB167394)

Immunohistochemical analysis of paraffin-embedded Human prostatic hyperplasia tissue, labeling AFM with ab167394 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (AB167394)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMF antibody [EPR11663(B)] (AB167394)

Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling AFM with ab167394 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

不同偶联物与剂型 (4)

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-AMF antibody [EPR11663(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-AMF antibody [EPR11663(B)]

  • 578 PE

    PE Anti-AMF antibody [EPR11663(B)]

  • Carrier free

    Anti-AMF antibody [EPR11663(B)] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR11663(B)

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

ICC/IF, WB, IHC-P, Flow Cyt (Intra)

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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为什么推荐这个？

我们推荐这个产品，因为它经常被用于相同的实验或相关的研究。

我们建议您务必查阅产品说明书，以确认其是否符合您的实验需求；若有疑问，也可联系我们的技术团队获取协助。

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式
Liquid
纯度
Tissue culture supernatant
存储溶液
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

In the cytoplasm, catalyzes the conversion of glucose-6-phosphate to fructose-6-phosphate, the second step in glycolysis, and the reverse reaction during gluconeogenesis (PubMed : 28803808). Besides it's role as a glycolytic enzyme, also acts as a secreted cytokine : acts as an angiogenic factor (AMF) that stimulates endothelial cell motility (PubMed : 11437381). Acts as a neurotrophic factor, neuroleukin, for spinal and sensory neurons (PubMed : 11004567, PubMed : 3352745). It is secreted by lectin-stimulated T-cells and induces immunoglobulin secretion (PubMed : 11004567, PubMed : 3352745).
See full target information GPI
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 26:5078-5094 PubMed36071546

2022

ISGylation of EMD promotes its interaction with PDHA to inhibit aerobic oxidation in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Congcong Zhang,Jiangtao Cui,Leiqun Cao,Xiaoting Tian,Yayou Miao,Yikun Wang,Shiyu Qiu,Wanxin Guo,Lifang Ma,Jinjing Xia,Xiao Zhang

Stem cell research & therapy 11:223 PubMed32513303

2020

Mesenchymal stem cell-derived exosomes exert ameliorative effects in type 2 diabetes by improving hepatic glucose and lipid metabolism via enhancing autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Qin He,Lingshu Wang,Ruxing Zhao,Fei Yan,Sha Sha,Chen Cui,Jia Song,Huiqing Hu,Xinghong Guo,Mengmeng Yang,Yixin Cui,Yujing Sun,Zheng Sun,Fuqiang Liu,Ming Dong,Xinguo Hou,Li Chen
View all publications
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。
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