重组Anti-AMBN抗体[EPR8317] (ab155950)
![Western blot - Anti-AMBN antibody [EPR8317] (ab155950)](https://www.abcam.cn/ps/products/155/ab155950/Images/ab155950-505355-anti-ambn-antibody-epr8317-western-blot-recombinant-protein.jpg "Western blot - Anti-AMBN antibody [EPR8317] (ab155950)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8317] to AMBN
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-AMBN抗体[EPR8317]
参阅全部 AMBN 一抗 -
描述
兔单克隆抗体[EPR8317] to AMBN -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Recombinant AMBN protein
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR8317 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab155950于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 48 kDa.
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| 说明 |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 48 kDa. |
靶标
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功能
Involved in the mineralization and structural organization of enamel. -
组织特异性
Ameloblast-specific. Located at the Tomes processes of secretory ameloblasts and in the sheath space between rod-interrod enamel. -
序列相似性
Belongs to the ameloblastin family. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 258 Human
- Omim: 601259 Human
- SwissProt: Q9NP70 Human
- Unigene: 272396 Human
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别名
- AMBN antibody
- AMBN_HUMAN antibody
- Ameloblastin (enamel matrix protein) antibody
- Ameloblastin antibody
图片
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Western blot - Anti-AMBN antibody [EPR8317] (ab155950)Lane 1 : Anti-AMBN antibody [EPR8317] (ab155950) at 1/1000 dilution
Lane 2 : Anti-GST antibody [EPR4236] (ab111947) at 1/1000 dilution
All lanes : Recombinant AMBN protein
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 48 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM /TBST.
Exposure time: Lane 1: 1 second. Lane 2: 20 seconds.
AMBN will migrate to higher MW size (PMID: 23782691), the MW is consistent with literature (PMID: 26070558).
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![Anti-AMBN antibody [EPR8317] (ab155950)](https://www.abcam.cn/ps/products/155/ab155950/Images/ab155950-2-benefits-of-recombinant-antibodies.png)
Anti-AMBN antibody [EPR8317] (ab155950)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab155950 尚未被引用在任何文献中。