重组Anti-ALS2CR8抗体[EPR13712] (ab180142)
![Flow Cytometry (Intracellular) - Anti-ALS2CR8 antibody [EPR13712] (ab180142)](https://www.abcam.cn/ps/products/180/ab180142/Images/ab180142-210026-ab1801422.jpg "Flow Cytometry (Intracellular) - Anti-ALS2CR8 antibody [EPR13712] (ab180142)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13712] to ALS2CR8
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-ALS2CR8抗体[EPR13712]
参阅全部 ALS2CR8 一抗 -
描述
兔单克隆抗体[EPR13712] to ALS2CR8 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human ALS2CR8 aa 100-300. The exact sequence is proprietary.
Database link: Q8N187 -
阳性对照
- SH-SY5Y and 293T cell lysate, SH-SY5Y cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR13712 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180142于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt (Intra) |
1/90.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
| WB |
1/5000 - 1/10000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
|
| 说明 |
|---|
|
Flow Cyt (Intra)
1/90. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/5000 - 1/10000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa). |
靶标
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功能
Acts as a transcriptional activator that mediates the calcium- and neuron-selective induction of BDNF exon III transcription. Binds to the consensus calcium-response element CaRE1 5'-CTATTTCGAG-3' sequence. -
结构域
The N-terminus is necessary for DNA-binding. The C-terminus is necessary for transcriptional activation (PubMed:11832226). -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 79800 Human
- Omim: 607586 Human
- SwissProt: Q8N187 Human
- Unigene: 444982 Human
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别名
- Amyotrophic lateral sclerosis 2 (juvenile) chromosome region candidate 8 antibody
- Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 8 protein antibody
- Calcium response factor antibody
see all
图片
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Flow Cytometry (Intracellular) - Anti-ALS2CR8 antibody [EPR13712] (ab180142)
Intracellular flow cytometric analysis of 2% paraformaldehyde fixed SH-SY5Y cells labeling ALS2CR8 with ab180142 at 1/90. Secondary antibody Goat anti rabbit IgG (FITC) at 1/75. Isotype control Rabbit IgG
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![Western blot - Anti-ALS2CR8 antibody [EPR13712] (ab180142)](https://www.abcam.cn/ps/products/180/ab180142/Images/ab180142-210024-ab180142.jpg)
Western blot - Anti-ALS2CR8 antibody [EPR13712] (ab180142)All lanes : Anti-ALS2CR8 antibody [EPR13712] (ab180142) at 1/10000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 81 kDa
Observed band size: 81 kDaBlocking buffer and concentration: 5% NFDM/TBST
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![Anti-ALS2CR8 antibody [EPR13712] (ab180142)](https://www.abcam.cn/ps/products/180/ab180142/Images/ab180142-3-benefits-of-recombinant-antibodies.png)
Anti-ALS2CR8 antibody [EPR13712] (ab180142)
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab180142 尚未被引用在任何文献中。