重组Anti-ALPK1抗体[EPR25006-61] (ab317807)
![Western blot - Anti-ALPK1 antibody [EPR25006-61] (ab317807)](https://www.abcam.cn/ps/products/317/ab317807/Images/ab317807-543735-anti-alpk1-antibody-epr25006-61-western-blot-wild-type-ibmm-immortalized-bone-marrow-derived-murine-macrophage-whole-cell-lysate-50-ug.jpg "Western blot - Anti-ALPK1 antibody [EPR25006-61] (ab317807)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25006-61] to ALPK1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-ALPK1抗体[EPR25006-61]
参阅全部 ALPK1 一抗 -
描述
兔单克隆抗体[EPR25006-61] to ALPK1 -
宿主
Rabbit -
特异性
This antibody only detects clear signal in iBMM (immortalized bone marrow-derived murine macrophage) whole cell lysate.
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经测试应用
适用于: WBmore details
不适用于: Flow Cyt (Intra),ICC/IF,IHC-P or IP -
种属反应性
与反应: Mouse
不与反应: Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Wild-type iBMM, P388D1, LADMAC whole cell lysates.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR25006-61 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317807于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000.
|
| 说明 |
|---|
|
WB
1/1000. |
靶标
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功能
Kinases that recognize phosphorylation sites in which the surrounding peptides have an alpha-helical conformation. -
组织特异性
Highly expressed in liver. -
序列相似性
Belongs to the protein kinase superfamily. Alpha-type protein kinase family. ALPK subfamily.
Contains 1 alpha-type protein kinase domain. - Information by UniProt
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数据库链接
- Entrez Gene: 71481 Mouse
- SwissProt: Q9CXB8 Mouse
- Unigene: 158704 Mouse
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别名
- 8430410J10Rik antibody
- Alpha protein kinase 1 antibody
- Alpha-protein kinase 1 antibody
see all
图片
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Western blot - Anti-ALPK1 antibody [EPR25006-61] (ab317807)All lanes : Anti-ALPK1 antibody [EPR25006-61] (ab317807) at 1/1000 dilution
Lane 1 : Wild-type iBMM (immortalized bone marrow-derived murine macrophage) whole cell lysate
Lane 2 : ALPK1 knockout iBMM whole cell lysate
Lane 3 : RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Mouse thymus tissue lysate
Lane 6 : Mouse testis tissue lysate
Lane 7 : P388D1 (mouse lymphoma monocyte; macrophage) whole cell lysate
Lane 8 : LADMAC (mouse bone marrow monocyte macrophage) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 136 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
In western blot, ab317807 was shown to bind specifically to ALPK1. Target of interest was observed at 140 kDa in wild-type iBMM cell lysates (lane 1) with no signal observed at this size in ALPK1 knockout cell line (lane 2).
The identity of the bands below the target band are unknown.
The wild-type iBMM and ALPK1 knockout iBMM lysates were kindly provided by Dr Feng Shao's lab, NIBS.
In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.
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![Anti-ALPK1 antibody [EPR25006-61] (ab317807)](https://www.abcam.cn/ps/products/317/ab317807/Images/ab317807-2-benefitsofrecombinantantibodies.jpg)
Anti-ALPK1 antibody [EPR25006-61] (ab317807)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
ab317807 尚未被引用在任何文献中。