重组Anti-alpha Tubulin (phospho Y272)抗体[EP1334(2)Y] (ab76290)
![Western blot - Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)](https://www.abcam.cn/ps/products/76/ab76290/Images/ab76290-550851-anti-alpha-tubulin-phospho-y272-antibody-ep1334-2-y-western-blot-human-jurkat.jpg "Western blot - Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1334(2)Y] to alpha Tubulin (phospho Y272)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-alpha Tubulin (phospho Y272)抗体[EP1334(2)Y]
参阅全部 alpha Tubulin 一抗 -
描述
兔单克隆抗体[EP1334(2)Y] to alpha Tubulin (phospho Y272) -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Jurkat cell lysates treated with pervanadate
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常规说明
This product has switched from a hybridoma to recombinant production method on 8th August 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1334(2)Y -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76290于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/50000. Predicted molecular weight: 54 kDa.
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| 说明 |
|---|
|
WB
1/50000. Predicted molecular weight: 54 kDa. |
应用说明
Is unsuitable for Flow Cyt,IHC-P or IP.
靶标
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功能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
序列相似性
Belongs to the tubulin family. -
翻译后修饰
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 7277 Human
- Omim: 191110 Human
- SwissProt: P68366 Human
- Unigene: 75318 Human
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别名
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
图片
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Western blot - Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290)All lanes : Anti-alpha Tubulin (phospho Y272) antibody [EP1334(2)Y] (ab76290) at 1/50000 dilution
Lane 1 : Untreated Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Jurkat treat with 10mM pervanadate for 30min whole cell lysate
Lane 3 : Jurkat treat with 10mM pervanadate for 30min, Then the membrane was incubated with alkaline phosphatase for 1 hour
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 40 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab76290 被引用在 3 文献中.
- Paech F et al. Hepatocellular Toxicity Associated with Tyrosine Kinase Inhibitors: Mitochondrial Damage and Inhibition of Glycolysis. Front Pharmacol 8:367 (2017). WB ; Human . PubMed: 28659801
- Yu Y et al. Inhibition of Spleen Tyrosine Kinase Potentiates Paclitaxel-Induced Cytotoxicity in Ovarian Cancer Cells by Stabilizing Microtubules. Cancer Cell 28:82-96 (2015). PubMed: 26096845
- Meshki J et al. Substance P induces rapid and transient membrane blebbing in U373MG cells in a p21-activated kinase-dependent manner. PLoS One 6:e25332 (2011). WB . PubMed: 21966499