Anti-alpha Tubulin抗体- Microtubule Marker (ab15246)
Key features and details
- Rabbit polyclonal to alpha Tubulin - Microtubule Marker
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-alpha Tubulin抗体- Microtubule Marker
参阅全部 alpha Tubulin 一抗 -
描述
兔多克隆抗体to alpha Tubulin - Microtubule Marker -
宿主
Rabbit -
特异性
Homology to both Tubulin alpha-3E chain and Tubulin alpha-4A chain
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经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Sheep, Rabbit, Chicken, Guinea pig, Cow, Pig, Xenopus laevis, Drosophila melanogaster, Zebrafish, Gerbil, Newt
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免疫原
Synthetic peptide within Human alpha Tubulin aa 400 to the C-terminus. The exact sequence is proprietary.
Database link: Q6PEY2 -
常规说明
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab15246于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (14) |
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
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| IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa). |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
序列相似性
Belongs to the tubulin family. -
翻译后修饰
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 7277 Human
- Entrez Gene: 22145 Mouse
- Entrez Gene: 316531 Rat
- Entrez Gene: 573122 Zebrafish
- Entrez Gene: 573216 Zebrafish
- Omim: 191110 Human
- SwissProt: P68366 Human
- SwissProt: P68368 Mouse
see all -
别名
- Alpha-tubulin 1 antibody
- ALS22 antibody
- B ALPHA 1 antibody
see all
图片
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Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)
Lanes: 1, 4, 7 - SK N BE; 2, 5, 8 - TE 671; 3, 6, 9 - HeLa
Lanes: 1-3: 4oC (1 freeze/thaw); 4-6: 4oC (2 freeze/thaws); 7-9 : 4oC (4 freeze/thaws)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)ab15246 at 1/200 dilution, staining alpha Tubulin in Human lung tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin- embedded tissue sections).
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Western blot - Anti-alpha Tubulin antibody - Microtubule Marker (ab15246)All lanes : Anti-alpha Tubulin antibody - Microtubule Marker (ab15246) at 1 µg/ml
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 50 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (274)
ab15246 被引用在 274 文献中.
- Menzele A et al. Hyperglycaemia-induced impairment of the autorhythmicity and gap junction activity of mouse embryonic stem cell-derived cardiomyocyte-like cells. Histochem Cell Biol 159:329-337 (2023). PubMed: 36547741
- Varela M et al. New Nitric Oxide-Releasing Compounds as Promising Anti-Bladder Cancer Drugs. Biomedicines 11:N/A (2023). PubMed: 36672707
- Afonso J et al. Glucose Metabolism Reprogramming in Bladder Cancer: Hexokinase 2 (HK2) as Prognostic Biomarker and Target for Bladder Cancer Therapy. Cancers (Basel) 15:N/A (2023). PubMed: 36765947
- Lee S et al. Melatonin loaded PLGA nanoparticles effectively ameliorate the in vitro maturation of deteriorated oocytes and the cryoprotective abilities during vitrification process. Biomater Sci 11:2912-2923 (2023). PubMed: 36883517
- Xiao Y et al. Dopey2 and Pcdh7 orchestrate the development of embryonic neural stem cells/ progenitors in zebrafish. iScience 26:106273 (2023). PubMed: 36936789