Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
重组RabMAb

重组Anti-alpha Tubulin抗体[EPR13478(B)] - Loading Control (ab176560)

Reviews (11) Submit a question References (43)
Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
  • Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13478(B)] to alpha Tubulin - Loading Control
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 405 Alexa Fluor® 488 Alexa Fluor® 647 APC Carrier Free HRP PE

概述

  • 产品名称

    Anti-alpha Tubulin抗体[EPR13478(B)] - Loading Control
    参阅全部 alpha Tubulin 一抗

  • 描述

    兔单克隆抗体[EPR13478(B)] to alpha Tubulin - Loading Control

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, African green monkey

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • HeLa, Jurkat, A431 and K562 cell lysates; Human kidney and uterus tissues; A431 and Jurkat cells.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab176560于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
1/60.

For unpurified, use 1/10.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB (8)
1/2000 - 1/10000. Predicted molecular weight: 50 kDa.

For unpurified, use 1/1000 - 1/10000.
IHC-P
1/350. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified, use 1/100.
ICC/IF (2)
1/350.

For unpurified, use 1/100.
说明
Flow Cyt (Intra)
1/60.

For unpurified, use 1/10.  ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/2000 - 1/10000. Predicted molecular weight: 50 kDa.

For unpurified, use 1/1000 - 1/10000.
IHC-P
1/350. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified, use 1/100.
ICC/IF
1/350.

For unpurified, use 1/100.

靶标

  • 功能

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

  • 序列相似性

    Belongs to the tubulin family.

  • 翻译后修饰

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

  • 细胞定位

    Cytoplasm > cytoskeleton.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

图片

  • Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560) at 1/7000 dilution (Purified) + Jurkat cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human uterus tissue labeling alpha Tubulin with unpurified ab176560 at a 1/100 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Immunohistochemical staining of paraffin embedded human kidney with purified ab176560 at a dilution of 1/350. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Immunofluorescent staining of A431 cells fixed in 4% PFA with purified ab176560 at a dilution of 1/350. An Alexa Fluor® 555 goat anti-rabbit was used as the secondary at a dilution of 1/500 and the sample was counter stained with DAPI. An Alexa Fluor® 555 goat anti-mouse was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.

  • Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Western blot - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    All lanes : Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560) at 1/1000 dilution (Unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 50 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Immunofluorescent staining of A431 cells fixed in 4% PFA with unpurified ab176560 at a dilution of 1/100. An Alexa Fluor® 555 goat anti-rabbit was used as the secondary at a dilution of 1/500 and the sample was counter stained with DAPI. An Alexa Fluor® 555 goat anti-mouse was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Immunohistochemical staining of paraffin embedded human kidney with unpurified ab176560 at a dilution of 1/100. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was counter stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Intracellular Flow Cytometry analysis of permeabilized HeLa cells labeling alpha Tubulin (pink) with purifiedab176560 at a 1/70 dilution, or negative control rabbit IgG (green). The secondary antibody was FITC goat anti-rabbit. 

     

     

  • Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Flow Cytometry (Intracellular) - Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

    Intracellular Flow Cytometry analysis of permeabilized Jurkat cells labeling alpha Tubulin (red) with unpurified ab176560 at a 1/10 dilution, or negative control rabbit IgG (green) 

     

     

  • Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)
    Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control (ab176560)

文献 (43)

发表研究结果有使用 ab176560？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab176560 被引用在 43 文献中.

  • Zhu W  et al. Integrated machine learning identifies epithelial cell marker genes for improving outcomes and immunotherapy in prostate cancer. J Transl Med 21:782 (2023). PubMed: 37925432
  • Wen J  et al. TUBA1A licenses APC/C-mediated mitotic progression to drive glioblastoma growth by inhibiting PLK3. FEBS Lett 597:3072-3086 (2023). PubMed: 37873730
  • Lin Z  et al. Inhibition of abnormal C/EBPβ/α-Syn signaling pathway through activation of Nrf2 ameliorates Parkinson's disease-like pathology. Aging Cell 22:e13958 (2023). PubMed: 37614147
  • Liu Z  et al. Hydrogel platform facilitating astrocytic differentiation through cell mechanosensing and YAP-mediated transcription. Mater Today Bio 22:100735 (2023). PubMed: 37576868
  • López-Guajardo A  et al. Regulation of cellular contractile force, shape and migration of fibroblasts by oncogenes and Histone deacetylase 6. Front Mol Biosci 10:1197814 (2023). PubMed: 37564130
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse vascular smooth muscle cells)
Permeabilization
Yes - NP40
Specification
Mouse vascular smooth muscle cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 11 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (U2OS)
Permeabilization
Yes - NP40
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 11 2021

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com