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Isotype/Loading Controls Loading Controls Tubulin

Anti-alpha Tubulin抗体[DM1A] - Loading Control (ab7291)

  • Datasheet
  • SDS
Reviews (90)Q&A (5)References (887)

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Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
  • Flow Cytometry - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
  • Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
  • Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

Key features and details

  • Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control
  • Suitable for: Flow Cyt, ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

Conjugates logo Related conjugates and formulations

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概述

  • 产品名称

    Anti-alpha Tubulin抗体[DM1A] - Loading Control
    参阅全部 alpha Tubulin 一抗
  • 描述

    小鼠单克隆抗体[DM1A] to alpha Tubulin - Loading Control
  • 宿主

    Mouse
  • 经测试应用

    适用于: Flow Cyt, ICC/IF, IHC-P, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
    预测可用于: Chicken, Guinea pig, Hamster, Cow, Dog, Pig, Xenopus laevis, Gerbil, African green monkey
  • 免疫原

    Full length native protein (purified) corresponding to Chicken alpha Tubulin.

  • 阳性对照

    • WB: HeLa, HEK293, HepG2, Caco2, NIH3T3, PC12 cell lysates. Flow Cyt: methanol fixed/Tween permeabilised HeLa cells. ICC/IF: Caco-2, NIH3T3, and SV40LT-SMC cells. IHC-P: Human colon and rat colon tissues.
  • 常规说明

    This antibody clone [DM1A] is manufactured by Abcam.

    Excellent as a protein loading control antibody. DM1A causes the 10 nm filaments to collapse into large lateral aggregates collecting in the cell periphery or tight juxtanuclear caps. It does not block microtubule assembly. It does not inhibit polymerisation or depolymerisation of platelet tubulin in vitro. It blocks (by 70-80%) the ability of tubulin dimers (with GppNHp bound) to promote a stable inhibition of adenylyl cyclase. See references for further information on the above.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 纯化说明

    Affinity-purified using protein G
  • Primary antibody说明

    Excellent as a protein loading control antibody.
  • 克隆

    单克隆
  • 克隆编号

    DM1A
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Isotype/Loading Controls
    • Loading Controls
    • Tubulin
    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • Tubulin
    • Isotype/Loading Controls
    • Loading Controls
    • Alpha Tubulin
    • Neuroscience
    • Processes

相关产品

  • Alternative Versions

    • Alexa Fluor® 680 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184093)
    • Alexa Fluor® 790 Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab184577)
    • Alexa Fluor® 488 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195887)
    • Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889)
    • Anti-alpha Tubulin antibody [DM1A] - BSA and Azide free (ab264493)
    • HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)
    • FITC Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab64503)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Related Products

    • Acetylated alpha Tubulin peptide (ab171288)
    • alpha Tubulin peptide (ab195584)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7291于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF (24)
Use a concentration of 0.5 - 1 µg/ml.
IHC-P (2)
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (60)
1/5000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

We recommend diluting ab7291 to 1:10000 and incubating overnight at 4°C. Works under both reducing and non-reducing conditions. We recommend using 3% BSA as the blocking agent, blocking with milk may cause a reduction in signal intensity.

说明
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF
Use a concentration of 0.5 - 1 µg/ml.
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/5000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

We recommend diluting ab7291 to 1:10000 and incubating overnight at 4°C. Works under both reducing and non-reducing conditions. We recommend using 3% BSA as the blocking agent, blocking with milk may cause a reduction in signal intensity.

靶标

  • 功能

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 序列相似性

    Belongs to the tubulin family.
  • 翻译后修饰

    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 细胞定位

    Cytoplasm > cytoskeleton.
  • Target information above from: UniProt accession P68366 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7277 Human
    • Entrez Gene: 22145 Mouse
    • Entrez Gene: 316531 Rat
    • Omim: 191110 Human
    • SwissProt: P68366 Human
    • SwissProt: P68368 Mouse
    • SwissProt: Q5XIF6 Rat
    • Unigene: 75318 Human
    • Unigene: 1155 Mouse
    • Unigene: 92961 Rat
    see all
  • 别名

    • Alpha-tubulin 1 antibody
    • ALS22 antibody
    • B ALPHA 1 antibody
    • bA408E5.3 antibody
    • H2 ALPHA antibody
    • Hum a tub1 antibody
    • Hum a tub2 antibody
    • LIS3 antibody
    • MGC171407 antibody
    • MGC55332 antibody
    • TBA4A_HUMAN antibody
    • Testis-specific alpha-tubulin antibody
    • TUBA1 antibody
    • TUBA1A antibody
    • tuba1l antibody
    • Tuba4a antibody
    • Tubulin alpha 1 chain antibody
    • Tubulin alpha antibody
    • Tubulin alpha-1 chain antibody
    • tubulin alpha-1B chain antibody
    • Tubulin alpha-4A chain antibody
    • Tubulin H2-alpha antibody
    • Tubulin, alpha 1 (testis specific) antibody
    • tubulin, alpha 1, like antibody
    • Tubulin, alpha 4a antibody
    • Tubulin, alpha, testis-specific antibody
    • Tubulin, alpha-1 antibody
    see all

图片

  • Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    All lanes : Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : SV40LT-SMC cell lysate
    Lane 4 : NIH/3T3 cell lysate
    Lane 5 : Rat liver tissue lysate
    Lane 6 : Rat heart tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) at 1/20000 dilution

    Performed under reducing conditions.

    Predicted band size: 50 kDa



    Merged signal (red and green). Green - ab7291 observed at 52 kDa. Red - loading control, ab181602, observed at 38 kDa.

    All samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab7291 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1/1,000 and 1/20,000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)Image from Dai C et al., PLoS One 10(8), Fig 5C. Doi: 10.1371/journal.pone.0063054. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    ab7291 staining alpha tubulin in human breast cancer cell line by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed 4% paraformaldehyde in PBS, permeabilized with 0.1% Triton X-100 in PBS  and blocked with 2% bovine serum albumin in sodium phosphate buffer. Cells were co-stained with anti-pericentrin using ab4448 at 1:500 dilution and ab7291 at 1:500 dilution. Alexa Fluor® 633 goat anti mouse and Alexa Fluor® 488 goat anti-rabbit (1:500 dilution) was used as secondary antibodies. DAPI was used as a nuclei counterstain. Representative images of mitotic cells with bipolar or multipolar spindles.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

    ab7291 staining alpha Tubulin in SV40LT-SMC cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab7291 at a working concentration of 0.5μg/ml and ab190573, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 647, shown in red) at 1/250 overnight at +4°C, followed by a further incubation at room temperature for 1h with an anti-mouse Alexa Fluor® 488 (ab150117) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    This product also gave a positive signal in 100% methanol (5 min) fixed SV40 cells under the same testing conditions.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

    IHC image of ab7291 staining alpha Tubulin in human colon formalin fixed paraffin embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7291, 5ug/ml working concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Flow Cytometry - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    Flow Cytometry - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

    Overlay histogram showing HeLa cells stained with ab7291 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab7291, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was an anti-mouse DyLight® 488 (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

    ab7291 staining alpha-Tubulin in Caco-2 cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab7291 at 1μg/ml and ab6046 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an anti-mouse Alexa Fluor® 488 (ab150117) at 2 μg/ml (shown in green) and anti-rabbit Alexa Fluor® 594 (ab150088) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.

    Negative controls:
    1 – Rabbit primary antibody and anti-mouse secondary antibody.
    2 – Mouse primary antibody and anti-rabbit secondary antibody.
    Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

    ab7291 staining alpha Tubulin in NIH3T3 cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab7291 at 1μl/ml and ab6046 at 1µg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an anti-mouse Alexa Fluor® 488 (ab150117) at 2 μg/ml (shown in green) and anti-rabbit Alexa Fluor® 594 (ab150088) at 2 μg/ml (shown in pseudo color red). Nuclear DNA was labelled in blue with DAPI.

    Negative controls:
    1 – Rabbit primary antibody and anti-mouse secondary antibody.
    2 – Mouse primary antibody and anti-rabbit secondary antibody.
    Controls 1 and 2 indicate that there is no unspecific reaction between primary and secondary antibodies used.

  • Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)This image is courtesy of an AbReview submitted by Elena Kashuba.
    All lanes : Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/2500 dilution

    Lanes 1-2 : Daudi (Human Burkitt's lymphoma cell line) at 10 µg
    Lanes 3-4 : Daudi (Human Burkitt's lymphoma cell line) at 15 µg
    Lanes 5-6 : Daudi (Human Burkitt's lymphoma cell line) at 20 µg

    Secondary
    All lanes : HRP conjugated monoclonal Goat Anti-Mouse IgG at 1/1000 dilution

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 1 minute

    See Abreview

  • Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    All lanes : Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma cell line) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 3 : PC12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa


    Exposure time: 150 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using an anti-mouse HRP (ab97040), and visualised using ECL development solution ab133406

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

    FABP4 (green) was detected using FABP4 primary antibody (ab92501; diluted 1/1000). Alpha tubulin (red) was detected using the mouse monoclonal (ab7291) antibody. Cells were imaged by confocal microscopy, using z-stack for adipocyte-like cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

    IHC image of ab7291 staining alpha Tubulin in rat colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7291, 0.5ug/ml working concentration, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

    ICC/IF image of ab7291 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab7291, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

    Immunofluorescent imaging of human cells (U2OS) with ab7291 reveals a delicate network of alpha-tubulin (green) located exclusively in the cytoplasm. The nucleus is stained blue.

    IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour.  Primary antibody used at 1/200 in 5% milk / 0.2% TWEEN for one hour, secondary antibody Alexa 488 for 30 minutes.  All blocking and incubation steps carried out at 37 degrees.

     

实验方案

  • Mouse on Mouse staining protocol

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (887)

发表研究结果有使用 ab7291?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7291 被引用在 887 文献中.

  • Brunetta HS  et al. Nitrate consumption preserves HFD-induced skeletal muscle mitochondrial ADP sensitivity and lysine acetylation: A potential role for SIRT1. Redox Biol 52:102307 (2022). WB ; Mouse . PubMed: 35398714
  • Bose A  et al. 14-3-3? prevents centrosome duplication by inhibiting NPM1 function. Genes Cells 26:426-446 (2021). PubMed: 33813791
  • Biswal MR  et al. Erythropoietin Gene Therapy Delays Retinal Degeneration Resulting from Oxidative Stress in the Retinal Pigment Epithelium. Antioxidants (Basel) 10:N/A (2021). PubMed: 34070383
  • Boone MA  et al. The FLI portion of EWS/FLI contributes a transcriptional regulatory function that is distinct and separable from its DNA-binding function in Ewing sarcoma. Oncogene 40:4759-4769 (2021). PubMed: 34145397
  • Maimon R  et al. A CRMP4-dependent retrograde axon-to-soma death signal in amyotrophic lateral sclerosis. EMBO J 40:e107586 (2021). PubMed: 34190355
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1-10 of 95 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (A549)
Permeabilization
Yes - Triton X-100 0.2%
Specification
A549
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
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提交于 Mar 10 2022

Immunocytochemistry abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
African green monkey Cultured Cells (Vero cells)
Permeabilization
Yes - Saponin 0.5%
Specification
Vero cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Mar 07 2022

Western blot abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (cardiac muscle)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
20 µg
Specification
cardiac muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Jan 26 2022

Western blot abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Pig Tissue lysate - whole (Dorsal Root Ganglion (DRG))
Gel Running Conditions
Reduced Denaturing (6%)
Loading amount
5 µg
Specification
Dorsal Root Ganglion (DRG)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Jan 17 2022

Western blot abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
28 µg
Specification
cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Nov 24 2021

Western blot abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
70 µg
Specification
cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Nov 17 2021

Western blot abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - nuclear (Cell line)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
58 µg
Specification
Cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Nov 16 2021

Western blot abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
120 µg
Specification
Cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Nov 16 2021

Western blot abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Caenorhabditis elegans Cell lysate - whole cell (Whole cell lysate rom N2 worms)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Whole cell lysate rom N2 worms
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Hannah Navarrete

Verified customer

提交于 Sep 06 2021

Western blot abreview for Anti-alpha Tubulin antibody [DM1A] - Loading Control

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Macrophage)
Gel Running Conditions
Reduced Non-Denaturing (Native) (10%)
Loading amount
20 µg
Treatment
1 µg/mL LPS for 18h
Specification
Macrophage
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Apr 02 2021

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