重组Anti-Alpha-synuclein (phospho Y39)抗体[EPR25118-5] (ab317366)
![Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366)](https://www.abcam.cn/ps/products/317/ab317366/Images/ab317366-539761-anti-alpha-synuclein-phospho-y39-antibody-epr25118-5-western-blot-293t-human-embryonic-kidney-epithelial-cell.jpg "Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR25118-5] to Alpha-synuclein (phospho Y39)
- Suitable for: IP, WB, Dot blot
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Alpha-synuclein (phospho Y39)抗体[EPR25118-5]
参阅全部 Alpha-synuclein 一抗 -
描述
兔单克隆抗体[EPR25118-5] to Alpha-synuclein (phospho Y39) -
宿主
Rabbit -
经测试应用
适用于: IP, WB, Dot blotmore details
不适用于: ICC/IF or IHC-P -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. -
阳性对照
- WB: 293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector IP: 293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector Dot: Human Alpha-synuclein (phospho Y39) peptide
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR25118-5 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317366于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
1/30.
|
|
| WB |
1/1000.
|
|
| Dot blot |
1/1000.
|
| 说明 |
|---|
|
IP
1/30. |
|
WB
1/1000. |
|
Dot blot
1/1000. |
靶标
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功能
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. -
组织特异性
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals. -
疾病相关
Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
序列相似性
Belongs to the synuclein family. -
结构域
The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. -
翻译后修饰
Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
细胞定位
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. - Information by UniProt
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数据库链接
- Entrez Gene: 6622 Human
- Omim: 163890 Human
- SwissProt: P37840 Human
- Unigene: 21374 Human
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别名
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
图片
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Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366)All lanes : Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366) at 1/1000 dilution
Lane 1 : 293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containi a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate (untreated membrane)
Lane 2 : 293T transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate (alkaline phosphatase treated membrane)
Lysates/proteins at 4 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 8 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-Alpha-synuclein antibody [EPR20535] (ab212184) staining at 1/1000 dilution.
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![Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366)](https://www.abcam.cn/ps/products/317/ab317366/Images/ab317366-539763-anti-alpha-synuclein-phospho-y39-antibody-epr25118-5-western-blot-293t-human-embryonic-kidney-epithelial-cell-with-wt.jpg)
Western blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366)All lanes : Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366) at 1/1000 dilution
Lane 1 : 293T (human embryonic kidney epithelial cell) transfected with humanTyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein (WT) expression vector containi a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate
Lane 2 : 293T transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein (mutant Y39) protein expression vector containi a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate
Lysates/proteins at 4 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
In Western blot, Anti-Alpha-synuclein antibody [EPR20535] (ab212184) staining at 1/1000 dilution.
In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
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![Immunoprecipitation - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366)](https://www.abcam.cn/ps/products/317/ab317366/Images/ab317366-1-anti-alpha-synuclein-phospho-y39-antibody-epr25118-5-immunoprecipitation-293t.jpg)
Immunoprecipitation - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366)Alpha-synuclein (phospho Y39) was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate with ab317366 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317366 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: 293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate
Lane 2: ab317366 IP in 293T (human embryonic kidney epithelial cell) transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317366 in 293T transfected with human Tyrosine-protein kinase ABL1 expression vector and a human Alpha-synuclein protein expression vector containing a myc-His-tag® treated with 1 mM pervanadate for 30 minutes whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
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![Dot Blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366)](https://www.abcam.cn/ps/products/317/ab317366/Images/ab317366-2-anti-alpha-synuclein-phospho-y39-antibody-epr25118-5-dot-blot-human-alpha-synuclein.jpg)
Dot Blot - Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366)Dot blot analysis of Alpha-synuclein (phospho Y39) using ab317366 at 1:1000 (0.504 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.
Lane 1: Human Alpha-synuclein (phospho Y39) peptide a
Lane 2: Human Alpha-synuclein (phospho Y39) peptide b
Lane 3: Human Alpha-synuclein non-phospho peptide
Lane 4: Human Alpha-synuclein (phospho S131) peptide
Lane 5: Human Alpha-synuclein non-phospho peptideExposure time: 180 seconds
Blocking buffer and concentration: 5% NFDM/TBST
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![Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366)](https://www.abcam.cn/ps/products/317/ab317366/Images/ab317366-3-benefitsofrecombinantantibodies.jpg)
Anti-Alpha-synuclein (phospho Y39) antibody [EPR25118-5] (ab317366)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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