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使用敲除细胞株进行验证

Anti-Alpha-synuclein抗体[syn204] (ab3309)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (9)

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Immunocytochemistry - Anti-Alpha-synuclein antibody [syn204] (ab3309)

    Key features and details

    • Mouse monoclonal [syn204] to Alpha-synuclein
    • Suitable for: ICC
    • Knockout validated
    • Reacts with: Human
    • Isotype: IgG2a

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    Goat Anti-Mouse IgG H&L (HRP) (ab205719)

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    概述

    • 产品名称

      Anti-Alpha-synuclein抗体[syn204]
      参阅全部 Alpha-synuclein 一抗
    • 描述

      小鼠单克隆抗体[syn204] to Alpha-synuclein
    • 宿主

      Mouse
    • 特异性

      Due to sequence homology ab3309 might react with Beta synuclein
    • 经测试应用

      适用于: ICCmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Recombinant full length protein corresponding to Human Alpha-synuclein.
      Database link: P37840

    • 阳性对照

      • ICC: HAP1 cells.
    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.40
      Preservative: 0.1% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • 纯度

      Protein G purified
    • 克隆

      单克隆
    • 克隆编号

      syn204
    • 同种型

      IgG2a
    • 轻链类型

      kappa
    • 研究领域

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Parkinson's disease
      • Synuclein
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Tangles & Tau
      • Neuroscience
      • Diseases
      • Neuroscience
      • Processes

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
    • Recombinant Protein

      • Recombinant Human Alpha-synuclein protein aggregate (Active) (ab218819)

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3309于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    ICC
    Use a concentration of 10 µg/ml.
    说明
    ICC
    Use a concentration of 10 µg/ml.

    靶标

    • 功能

      May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation.
    • 组织特异性

      Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals.
    • 疾病相关

      Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
      Parkinson disease 1
      Parkinson disease 4
      Dementia Lewy body
    • 序列相似性

      Belongs to the synuclein family.
    • 结构域

      The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments.
    • 翻译后修饰

      Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
      Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
      Ubiquitinated. The predominant conjugate is the diubiquitinated form.
      Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure.
    • 细胞定位

      Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons.
    • Target information above from: UniProt accession P37840 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 6622 Human
      • Omim: 163890 Human
      • SwissProt: P37840 Human
      • Unigene: 21374 Human
      • 别名

        • Alpha synuclein antibody
        • Alpha-synuclein antibody
        • Alpha-synuclein, isoform NACP140 antibody
        • alphaSYN antibody
        • MGC105443 antibody
        • MGC110988 antibody
        • MGC127560 antibody
        • MGC64356 antibody
        • NACP antibody
        • Non A beta component of AD amyloid antibody
        • Non A4 component of amyloid antibody
        • Non A4 component of amyloid precursor antibody
        • Non-A beta component of AD amyloid antibody
        • Non-A-beta component of alzheimers disease amyloid , precursor of antibody
        • Non-A4 component of amyloid precursor antibody
        • Non-A4 component of amyloid, precursor of antibody
        • OTTHUMP00000218549 antibody
        • OTTHUMP00000218551 antibody
        • OTTHUMP00000218552 antibody
        • OTTHUMP00000218553 antibody
        • OTTHUMP00000218554 antibody
        • PARK 1 antibody
        • PARK 4 antibody
        • PARK1 antibody
        • PARK4 antibody
        • Parkinson disease (autosomal dominant, Lewy body) 4 antibody
        • Parkinson disease familial 1 antibody
        • SNCA antibody
        • Snca synuclein antibody
        • Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
        • SYN antibody
        • Synuclein alpha antibody
        • Synuclein alpha 140 antibody
        • Synuclein, alpha (non A4 component of amyloid precursor) antibody
        • SYUA_HUMAN antibody
        see all

      图片

      • Immunocytochemistry - Anti-Alpha-synuclein antibody [syn204] (ab3309)
        Immunocytochemistry - Anti-Alpha-synuclein antibody [syn204] (ab3309)
        ab3309 staining Alpha-Synuclein in wild-type Hap1 cells (top panel) and SNCA knockout Hap1 cells (bottom panel). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab3309 at 10μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
        Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

      实验方案

      • Western blot protocols
      • Immunoprecipitation protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (9)

      发表研究结果有使用 ab3309?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab3309 被引用在 9 文献中.

      • Park HJ  et al. Ethanol extract from Gynostemma pentaphyllum ameliorates dopaminergic neuronal cell death in transgenic mice expressing mutant A53T human alpha-synuclein. Neural Regen Res 15:361-368 (2020). PubMed: 31552910
      • Noonong K  et al. Neuroprotective and Neurorestorative Effects of Holothuria scabra Extract in the MPTP/MPP+-Induced Mouse and Cellular Models of Parkinson's Disease. Front Neurosci 14:575459 (2020). PubMed: 33408606
      • Agerschou ED  et al. An engineered monomer binding-protein for a-synuclein efficiently inhibits the proliferation of amyloid fibrils. Elife 8:N/A (2019). PubMed: 31389332
      • Nevzglyadova OV  et al. Yeast red pigment modifies cloned human a-synuclein pathogenesis in Parkinson disease models in Saccharomyces cerevisiae and Drosophila melanogaster. Neurochem Int 120:172-181 (2018). PubMed: 30099122
      • Ma L  et al. C-terminal truncation exacerbates the aggregation and cytotoxicity of a-Synuclein: A vicious cycle in Parkinson's disease. Biochim Biophys Acta Mol Basis Dis 1864:3714-3725 (2018). PubMed: 30290273
      • Shahnawaz M  et al. Development of a Biochemical Diagnosis of Parkinson Disease by Detection of a-Synuclein Misfolded Aggregates in Cerebrospinal Fluid. JAMA Neurol 74:163-172 (2017). PubMed: 27918765
      • Bassil F  et al. Reducing C-terminal truncation mitigates synucleinopathy and neurodegeneration in a transgenic model of multiple system atrophy. Proc Natl Acad Sci U S A 113:9593-8 (2016). SDS-PAGE . PubMed: 27482103
      • Volpicelli-Daley LA  et al. Addition of exogenous a-synuclein preformed fibrils to primary neuronal cultures to seed recruitment of endogenous a-synuclein to Lewy body and Lewy neurite-like aggregates. Nat Protoc 9:2135-46 (2014). PubMed: 25122523
      • Croisier E  et al. Comparative study of commercially available anti-alpha-synuclein antibodies. Neuropathol Appl Neurobiol 32:351-6 (2006). PubMed: 16640654

      客户评价及客户问答

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      1-2 of 2 Abreviews or Q&A

      Question

      Thank you very much. Could you please let me know whether I can use PBS buffer as antibody diluent( my second query of ref:914295)

      Read More

      Abcam community

      Verified customer

      Asked on Oct 04 2006

      Answer

      Thank you for getting back to me. Phosphate buffered saline can be used as an antibody diluent as an alternative to TBS for western blot analysis. However, with regards storage I would recommend that the antibodies are alituotted and frozen at -20oC upon arrival without prior dilution. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      回复于 Oct 05 2006

      Question

      Do you know this antibody's epitope, ie what stretch of amino acids of synuclein that it binds to?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 13 2003

      Answer

      No I am afraid we do not know the epitope for this product, we will update the on line datasheet if we receive any further information.

      Read More

      Asdf Edo

      Abcam Scientific Support

      回复于 Oct 13 2003

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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