Anti-Alpha-synuclein抗体[LB 509] (ab27766)
![Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766)](https://www.abcam.cn/ps/products/27/ab27766/Images/ab27766-278136-anti-alpha-synuclein-antibody-lb-509-western-blot.jpg "Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766)")
Key features and details
- Mouse monoclonal [LB 509] to Alpha-synuclein
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG1
概述
-
产品名称
Anti-Alpha-synuclein抗体[LB 509]
参阅全部 Alpha-synuclein 一抗 -
描述
小鼠单克隆抗体[LB 509] to Alpha-synuclein -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cyt (Intra)more details -
种属反应性
与反应: Rat, Human, Recombinant fragment -
免疫原
Full length protein. This information is proprietary to Abcam and/or its suppliers.
-
表位
ab27766 reacts with an epitope located in the region encoded by amino acids 115-122 of alpha-synuclein. -
阳性对照
- WB: Recombinant human Alpha-synuclein protein. Flow Cyt (Intra): PC12 (NGF differentiated) cells.
-
常规说明
Alpha-synuclein is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals. The deposition of the abundant presynaptic brain protein alpha-synuclein as fibrillary aggregates in neurons or glial cells is a hallmark lesion in a subset of neurodegenerative disorders. These disorders include Parkinson's disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy, collectively referred to as synucleinopathies. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
纯度
Protein G purified -
克隆
单克隆 -
克隆编号
LB 509 -
同种型
IgG1 -
研究领域
相关产品
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27766于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
1/100 - 1/1000. Predicted molecular weight: 14 kDa.
|
| Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| 说明 |
|---|
|
WB
1/100 - 1/1000. Predicted molecular weight: 14 kDa. |
|
Flow Cyt (Intra)
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
靶标
-
功能
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. -
组织特异性
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals. -
疾病相关
Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
序列相似性
Belongs to the synuclein family. -
结构域
The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. -
翻译后修饰
Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
细胞定位
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. - Information by UniProt
-
数据库链接
- Entrez Gene: 6622 Human
- Entrez Gene: 29219 Rat
- Omim: 163890 Human
- SwissProt: P37840 Human
- SwissProt: P37377 Rat
- Unigene: 21374 Human
- Unigene: 1827 Rat
-
别名
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
图片
-
Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766)All lanes : Anti-Alpha-synuclein antibody [LB 509] (ab27766) at 5 µg/ml
Lane 1 :Recombinant Human Alpha-synuclein protein (ab51189)
Lane 2 : Human brain hippocampus tissue lysate - total protein (ab30180)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 16 kDa why is the actual band size different from the predicted?
Additional bands at: 32 kDa (possible dimer)
Exposure time: 20 minutes -
![Flow Cytometry (Intracellular) - Anti-Alpha-synuclein antibody [LB 509] (ab27766)](https://www.abcam.cn/ps/products/27/ab27766/Images/ab27766-282150-anti-alpha-synuclein-antibody-lb-509-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Alpha-synuclein antibody [LB 509] (ab27766)Overlay histogram showing PC12 (NGF differentiated) cells stained with ab27766 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab27766, 1µg/1x106) for 30 min at 22°C. The secondary antibody used was Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.
Isotype control antibody (black line) was Mouse IgG1 [15-6E10A7] (ab170190) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW Blue laser (488nm) and 530/30 bandpass filter.
-
![Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766)](https://www.abcam.cn/ps/products/27/ab27766/Images/ab27766-277988-anti-alpha-synuclein-antibody-lb-509-western-blot.jpg)
Western blot - Anti-Alpha-synuclein antibody [LB 509] (ab27766)Anti-Alpha-synuclein antibody [LB 509] (ab27766) at 1/1000 dilution +Recombinant Human Alpha-synuclein protein (ab51189) at 0.1 µg
Secondary
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 5000 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Exposure time: 8 minutes
数据表及文件
-
SDS download
-
Datasheet download
文献 (81)
ab27766 被引用在 81 文献中.
- Singh Y et al. Overexpression of human alpha-Synuclein leads to dysregulated microbiome/metabolites with ageing in a rat model of Parkinson disease. Mol Neurodegener 18:44 (2023). PubMed: 37403161
- Pieger K et al. Translocation of Distinct Alpha Synuclein Species from the Nucleus to Neuronal Processes during Neuronal Differentiation. Biomolecules 12:N/A (2022). PubMed: 36009004
- Stojkovska I et al. Rescue of α-synuclein aggregation in Parkinson's patient neurons by synergistic enhancement of ER proteostasis and protein trafficking. Neuron 110:436-451.e11 (2022). PubMed: 34793693
- Kim C et al. Efficacy and immunogenicity of MultiTEP-based DNA vaccines targeting human α-synuclein: prelude for IND enabling studies. NPJ Vaccines 7:1 (2022). PubMed: 35013319
- Mazzetti S et al. Astrocytes expressing Vitamin D-activating enzyme identify Parkinson's disease. CNS Neurosci Ther 28:703-713 (2022). PubMed: 35166042