Anti-Alpha-synuclein抗体(ab6162)

Thank you for calling Abcam yesterday.

I have heard back from the lab about the buffer of ab6162. All of our affinity purified antibody ultimately end up in PBS from which they are then lyophilized, thus all that is remaining are some buffer salts and antibody protein once dried. Initial composition was 50mM Na+Phosphate, 150mM NaCL, final pH 7.5. There are no preservatives.

I hope this helps, please let me know if there is anything else I can help you with.

Thank you very much for your enquiry.

Unfortunately, we currently do not have a particular ready-to-useIHC Kit available that has been optimised for the use of a sheep polyclonal primary antibody with rat tissue.

Therefore, if you do not have the appropriate components available,I may recommend the individual components for IHC which are listed in more detail at https://www.abcam.com/index.html?pageconfig=resource&rid=12853.

For example, I would suggest one of the secondary antibodiesab6747 or ab97125:
https://www.abcam.com/Rabbit-Sheep-IgG-HL-HRP-ab6747.html (or use the following: https://www.abcam.com/Rabbit-Sheep-IgG-HL-HRP-ab6747.html).
https://www.abcam.com/Donkey-Sheep-IgG-HL-HRP-ab97125.html (or use the following: https://www.abcam.com/Donkey-Sheep-IgG-HL-HRP-ab97125.html).

As you may see, we also have
buffers (https://www.abcam.com/index.html?pageconfig=resource&rid=12854),
diluents (https://www.abcam.com/index.html?pageconfig=resource&rid=12864),
blocking solutions/serum (https://www.abcam.com/index.html?pageconfig=resource&rid=12859or https://www.abcam.com/index.html?pageconfig=resource&rid=12889)
and substrates (https://www.abcam.com/index.html?pageconfig=resource&rid=12908or https://www.abcam.com/index.html?pageconfig=resource&rid=12886)
for you to tailor your specific experiments with rat brain sectionsavailable.

These include for example the following products:

ab64214: https://www.abcam.com/10x-Citrate-Buffer-pH-60-ab64214.html (or use the following: https://www.abcam.com/10x-Citrate-Buffer-pH-60-ab64214.html).

ab64216: https://www.abcam.com/10x-EDTA-Buffer-pH-80-ab64216.html (or use the following: https://www.abcam.com/10x-EDTA-Buffer-pH-80-ab64216.html).

ab64211: https://www.abcam.com/Antibody-Diluent-ab64211.html (or use the following: https://www.abcam.com/Antibody-Diluent-ab64211.html).

ab64226: https://www.abcam.com/Protein-Block-ab64226.html (or use the following: https://www.abcam.com/Protein-Block-ab64226.html).

ab64238: https://www.abcam.com/DAB-Substrate-Kit-ab64238.html (or use the following: https://www.abcam.com/DAB-Substrate-Kit-ab64238.html).

Also, I have attached our protocols for your consideration.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

The production and formulation of this sheep antibody to alpha synuclein has not changed since 2009. I hope this is helpful. Please contact us again if you have any further questions.

We assume you are staining for Lewy bodies in human diseased brains. We are using this antibody every week. The staining of Lewy bodies is really quite superb, either in paraffin or cryostat sections. The human tissue is fixed in Zamboni's fixative (4% formaldehyde with 15% saturated picric acid) and used without antigen retrieval. Occassionally we have found some minor improvement using antigen retrieval in 0.1M citrate buffer pH 6.0 improves staining but we rarely use it as staining is always clear.

This antibody works very well on paraffin sections. The antibody will need to be used at a concentration a little higher than for cryostat sections, about 1/500 to 1/1000.