Anti-Alpha-synuclein抗体(ab6162)
Key features and details
- Sheep polyclonal to Alpha-synuclein
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Alpha-synuclein抗体
参阅全部 Alpha-synuclein 一抗 -
描述
羊多克隆抗体to Alpha-synuclein -
宿主
Sheep -
特异性
Human and rat alpha synuclein. Cross reactivity: This antibody is known to react with human, mouse, rat and other rodents. Cross reactivity with other species has not yet been tested. -
经测试应用
适用于: WBmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human Alpha-synuclein aa 116-131. Synthetic peptide of human synuclein (116-131) coupled to diphtheria toxoid with cysteine residue attached to N-ter of peptide. Conjugate cross-linked by maleimidocaproyl-N-hydroxy succinimide (MSC).
Sequence:C-MPVDPDNEAYEMPSEE
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 0.5 µg/ml.
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说明 |
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WB
Use a concentration of 0.5 µg/ml. |
靶标
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功能
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. -
组织特异性
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals. -
疾病相关
Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
序列相似性
Belongs to the synuclein family. -
结构域
The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. -
翻译后修饰
Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
细胞定位
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. - Information by UniProt
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数据库链接
- Entrez Gene: 6622 Human
- Entrez Gene: 20617 Mouse
- Omim: 163890 Human
- SwissProt: P37840 Human
- SwissProt: O55042 Mouse
- Unigene: 21374 Human
- Unigene: 17484 Mouse
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别名
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (28)
ab6162 被引用在 28 文献中.
- Yin S et al. Cancerous Inhibitor of Protein Phosphatase 2A (CIP2A): Could It Be a Promising Biomarker and Therapeutic Target in Parkinson's Disease? Mol Neurobiol 59:1333-1344 (2022). PubMed: 34984583
- Chen Y et al. Increased Accumulation of α-Synuclein in Inflamed Appendices of Parkinson's Disease Patients. Mov Disord 36:1911-1918 (2021). PubMed: 33876851
- Komuro Y et al. The Role of Galanin in Cerebellar Granule Cell Migration in the Early Postnatal Mouse during Normal Development and after Injury. J Neurosci 41:8725-8741 (2021). PubMed: 34462307
- Zhang D et al. Microglial activation contributes to cognitive impairments in rotenone-induced mouse Parkinson's disease model. J Neuroinflammation 18:4 (2021). PubMed: 33402167
- Han C et al. Exosomes from patients with Parkinson's disease are pathological in mice. J Mol Med (Berl) 97:1329-1344 (2019). PubMed: 31302715