AB190376

Anti-Alpha-synuclein抗体

Name: Alpha-synuclein抗体 (ab190376)| Abcam中文官网
Brand: Abcam Limited
SKU: AB190376
Price: 4869 CNY
Availability: InStock

Anti-Alpha-synuclein antibody

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(4 Publications)

Chicken Polyclonal Alpha-synuclein antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human SNCA.

查看别名

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (AB190376)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (AB190376)

Chromogenic immunostaining of a formalin fixed paraffin embedded human cerebellum section with chicken pAb to α-synuclein, dilution 1/5000, detected in DAB (brown) following the ABC method. Hematoxylin (blue) was used as the counterstain. The α-synuclein protein is concentrated in synapses, in particular highlighting the glomeruli synapses in the granule layer and those on Purkinje dendrites in the molecular layer.

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein antibody (AB190376)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein antibody (AB190376)

Immunofluorescent analysis of Mixed rat neuron-glial cultures labeling alpha Synuclein with ab190376 at 1/1000 dilution (red) and and monoclonal antibody to MAP2 (green); DNA (blue).

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (AB190376)

ab190376 staining alpha-synuclein in rat cerebellum tissue sections at 1/3000 dilution (red) and co-stained with rabbit polyclonal antibody to GFAP. DAPI was used as a nuclear counterstain.

Supplier Data

Western blot - Anti-Alpha-synuclein antibody (AB190376)

All lanes:

Western blot - Anti-Alpha-synuclein antibody (ab190376) at 1/2000 dilution

All lanes:

Rat brain crude extract

Predicted band size: 14 kDa

false

关键信息

宿主种属

Chicken

克隆

Polyclonal

亚型

IgY

不含载体蛋白

反应种属

Rat

应用

ICC/IF, IHC-P, WB

applications

免疫原

Recombinant Full Length Protein corresponding to Human SNCA.

P37840

特异性

Due to high sequence homology ab190376 might react with Beta synuclein

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/3000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯度

IgY fraction

存储溶液

Preservative: 0.0325% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed : 20798282, PubMed : 26442590, PubMed : 28288128, PubMed : 30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed : 28288128, PubMed : 30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed : 30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed : 20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed : 26442590).

See full target information SNCA

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed39147589

2024

Heterogeneity in Slow Synaptic Transmission Diversifies Purkinje Cell Timing.

Applications

Unspecified application

Species

Unspecified reactive species

Riya Elizabeth Thomas,Franziska Mudlaff,Kyra Schweers,William Todd Farmer,Aparna Suvrathan

NPJ Parkinson's disease 10:1 PubMed38167744

2024

Aggregate-prone brain regions in Parkinson's disease are rich in unique N-terminus α-synuclein conformers with high proteolysis susceptibility.

Applications

Unspecified application

Species

Unspecified reactive species

James A Wiseman,Helen C Murray,Richard L M F Faull,Michael Dragunow,Clinton P Turner,Birger Victor Dieriks,Maurice A Curtis

Cell reports 41:111852 PubMed36543134

2022

MED13 and glycolysis are conserved modifiers of α-synuclein-associated neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Mengda Ren,Ying Yang,Kelsey Hwee Yee Heng,Lu Yi Ng,Claris Yuin-Yi Chong,Yan Ting Ng,Srinivas Gorur-Shandilya,Rachel Min Qi Lee,Kah Leong Lim,Jing Zhang,Tong-Wey Koh

Annals of neurology 92:230-245 PubMed35596947

2022

Parkinson's Disease Derived Exosomes Aggravate Neuropathology in SNCA*A53T Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Huang,Zongran Liu,Na Li,Chen Tian,Han Yang,Yanfei Huo,Yang Li,Jing Zhang,Zhenwei Yu

View all publications

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Anti-Alpha-synuclein抗体

Anti-Alpha-synuclein antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (AB190376)

Immunocytochemistry/ Immunofluorescence - Anti-Alpha-synuclein antibody (AB190376)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody (AB190376)

Western blot - Anti-Alpha-synuclein antibody (AB190376)

关键信息

宿主种属

克隆

亚型

不含载体蛋白

反应种属

应用

免疫原

特异性