Anti-Alpha-synuclein抗体[4D6] - BSA and Azide free (ab1903)
![Western blot - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903)](https://www.abcam.cn/ps/products/1/ab1903/Images/ab1903-5206-anti-alpha-synuclein-antibody-4d6-western-blot.jpg "Western blot - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903)")
Key features and details
- Mouse monoclonal [4D6] to Alpha-synuclein - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-Alpha-synuclein抗体[4D6] - BSA and Azide free
参阅全部 Alpha-synuclein 一抗 -
描述
小鼠单克隆抗体[4D6] to Alpha-synuclein - BSA and Azide free -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt or ICC -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human Alpha-synuclein.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
Ammonium sulfate precipitated and dialyzed tissue culture supernatant. -
克隆
单克隆 -
克隆编号
4D6 -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (3) |
1/100 - 1/10000. Predicted molecular weight: 16 kDa.
|
| IHC-P | (1) |
1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining.
|
| 说明 |
|---|
|
WB
1/100 - 1/10000. Predicted molecular weight: 16 kDa. |
|
IHC-P
1/100 - 1/1000. Antigen retrieval is not essential but may optimise staining. |
应用说明
Is unsuitable for Flow Cyt or ICC.
靶标
-
功能
May be involved in the regulation of dopamine release and transport. Induces fibrillization of microtubule-associated protein tau. Reduces neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. -
组织特异性
Expressed principally in brain but is also expressed in low concentrations in all tissues examined except in liver. Concentrated in presynaptic nerve terminals. -
疾病相关
Genetic alterations of SNCA resulting in aberrant polymerization into fibrils, are associated with several neurodegenerative diseases (synucleinopathies). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimer disease amyloid plaque, and a major component of Lewy body inclusions. They are also found within Lewy body (LB)-like intraneuronal inclusions, glial inclusions and axonal spheroids in neurodegeneration with brain iron accumulation type 1.
Parkinson disease 1
Parkinson disease 4
Dementia Lewy body -
序列相似性
Belongs to the synuclein family. -
结构域
The 'non A-beta component of Alzheimer disease amyloid plaque' domain (NAC domain) is involved in fibrils formation. The middle hydrophobic region forms the core of the filaments. The C-terminus may regulate aggregation and determine the diameter of the filaments. -
翻译后修饰
Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress.
Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers.
Ubiquitinated. The predominant conjugate is the diubiquitinated form.
Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. -
细胞定位
Cytoplasm, cytosol. Membrane. Nucleus. Cell junction, synapse. Secreted. Membrane-bound in dopaminergic neurons. - Information by UniProt
-
数据库链接
- Entrez Gene: 6622 Human
- Omim: 163890 Human
- SwissProt: P37840 Human
- Unigene: 21374 Human
-
别名
- Alpha synuclein antibody
- Alpha-synuclein antibody
- Alpha-synuclein, isoform NACP140 antibody
see all
图片
-
Western blot - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903)All lanes : Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903) at 5 µg/ml
Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Additional bands at: 18 kDa (possible post-translational modification), 80 kDa (possible post-translational modification) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903)](https://www.abcam.cn/ps/products/1/ab1903/Images/ab1903-5207-anti-alpha-synuclein-antibody-4d6-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903)IHC-P using ab1903 showing lewy bodies in a human substantia nigra neuron(x400)
数据表及文件
-
Datasheet download
文献 (57)
ab1903 被引用在 57 文献中.
- Niu M et al. VPS35 D620N knockin mice recapitulate cardinal features of Parkinson's disease. Aging Cell 20:e13347 (2021). PubMed: 33745227
- Threlfell S et al. Striatal Dopamine Transporter Function Is Facilitated by Converging Biology of a-Synuclein and Cholesterol. Front Cell Neurosci 15:658244 (2021). PubMed: 33935654
- Bengoa-Vergniory N et al. CLR01 protects dopaminergic neurons in vitro and in mouse models of Parkinson's disease. Nat Commun 11:4885 (2020). PubMed: 32985503
- Heo JY et al. Aberrant Tonic Inhibition of Dopaminergic Neuronal Activity Causes Motor Symptoms in Animal Models of Parkinson's Disease. Curr Biol 30:276-291.e9 (2020). PubMed: 31928877
- Cheng J et al. Microglial autophagy defect causes parkinson disease-like symptoms by accelerating inflammasome activation in mice. Autophagy 16:2193-2205 (2020). PubMed: 32003282
