AB1903

Anti-Alpha-synuclein抗体[4D6] - BSA and Azide free

Name: Alpha-synuclein抗体[4D6] - BSA and Azide free (ab1903)| Abcam中文官网
Brand: Abcam Limited
SKU: AB1903
Price: 4430 CNY
Availability: InStock
Rating: 4 (4 reviews)

Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free

(4 Reviews)

(99 Publications)

Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903) is a mouse monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Alpha-synuclein in Western Blot, IHC-P. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Over 70 publications
- Trusted since 2004

查看别名

NACP, PARK1, SNCA, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor

2 Images

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Western blot - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (AB1903)

All lanes:

Western blot - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903) at 5 µg/mL

Lane 1:

A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate at 10 µg

Lane 2:

SK N BE (Human neuroblastoma) Whole Cell Lysate at 10 µg

Lane 3:

SK N SH (Human neuroblastoma) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 14 kDa

Observed band size: 16 kDa,18 kDa,80 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (AB1903)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (AB1903)

IHC-P using ab1903 showing lewy bodies in a human substantia nigra neuron(x400)

不同偶联物与剂型 (2)

Carrier free

Anti-CRBN antibody [4D6] - BSA and Azide free
Unconjugated

Anti-CRBN antibody [4D6]

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

4D6

亚型

IgG1

不含载体蛋白

Yes

反应种属

Human

应用

IHC-P, WB

applications

免疫原

Recombinant Full Length Protein corresponding to Human SNCA.

P37840

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/10000", "WB-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody." } } }

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产品详情

What is this antibody validated in?
Anti-Alpha-synuclein antibody [4D6] - BSA and Azide free (ab1903) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Alpha-synuclein?
Anti-Alpha-synuclein [4D6] - BSA and Azide free (ab1903) specifically detects a band for Alpha-synuclein (UniProt: P37840) at a molecular weight of 16kDa.

Trusted by the scientific community
Anti-Alpha-synuclein [4D6] - BSA and Azide free (ab1903) was first used in a scientific publication in 2004 and has been cited over 70 times in peer-reviewed journals.

Reviewed by scientists
Anti-Alpha-synuclein [4D6] - BSA and Azide free (ab1903) has over 5 independent reviews from customers.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

纯化说明

Ammonium sulfate precipitated and dialyzed tissue culture supernatant.

存储溶液

Constituents: PBS

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-synuclein often referred to by alternate names such as SNCA is a protein of around 14 kDa mass. It mainly expresses in the brain particularly in presynaptic nerve terminals. This protein functions mechanically by stabilizing synaptic vesicles and maintaining synaptic function. It exists both in soluble monomer forms and as aggregates in protein filaments. Antibodies like 4D6 and EP1536Y target monomer forms of protein for more detailed studies.

Biological function summary

The alpha-synuclein protein plays critical roles in neuronal activity. It contributes to neurotransmitter release regulation by acting in the formation and plasticity of the presynaptic neuronal network. Alpha-synuclein doesn't usually form parts of large protein complexes but it may associate transiently with membranes and vesicular structures. The protein's monomer form has also been observed in alpha lines and related neuronal processes operating alongside various cellular functions.

Pathways

Synaptic vesicle trafficking and dopamine neurotransmitter release are significant areas involving the alpha-synuclein protein. In these pathways alpha-synuclein interacts with other proteins like synaptophysin and protein monomer monomerizations are intrinsic to these processes. Altered function or aggregation of alpha-synuclein disrupts these pathways influencing broader neurological functions.

Alterations or accumulations of alpha-synuclein are strongly linked to Parkinson's disease and Lewy body dementia. In these conditions alpha-synuclein forms abnormal protein filaments known as Lewy bodies within neurons. These formations disrupt cellular processes and neuron health. Synucleinopathies such as these show connections with proteins like parkin and DJ-1 which also have key roles in these neurodegenerative diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release (PubMed : 20798282, PubMed : 26442590, PubMed : 28288128, PubMed : 30404828). Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores (PubMed : 28288128, PubMed : 30404828). Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis (PubMed : 30404828). Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5 (PubMed : 20798282). This chaperone activity is important to sustain normal SNARE-complex assembly during aging (PubMed : 20798282). Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity (PubMed : 26442590).

See full target information SNCA

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文献 (99)

Recent publications for all applications. Explore the full list and refine your search

Translational neurodegeneration 14:26 PubMed40420149

2025

Development of human targeted extracellular vesicles loaded with shRNA minicircles to prevent parkinsonian pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Izco,Carlos Sola,Martin Schleef,Marco Schmeer,María de Toro,Guglielmo Verona,Estefania Carlos,Alejandro Reinares-Sebastian,Sandra Colina,Maria Eugenia Marzo-Sola,Josune Garcia-Sanmartin,Joaquín Fernández-Irigoyen,Enrique Santamaría,Rodolfo Mugica-Vidal,Javier Blesa,Lydia Alvarez-Erviti

Journal of neurology 272:299 PubMed40156621

2025

PRNP E146G mutation inherited prion disease: distinctive clinical, pathological and fluid biomarker features.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Coysh,Zane Jaunmuktane,Laszlo L P Hosszu,Nour Majbour,Fuquan Zhang,Tracy Campbell,Lee Darwent,Marcelo Barria Matus,Edgar Chan,Leah Holm-Mercer,Tze How Mok,Jonathan D F Wadsworth,Jan Bieschke,Kannan Nithi,Sebastian Brandner,Colin Smith,Margaret Esiri,John Collinge,Simon Mead

NPJ Parkinson's disease 11:47 PubMed40089519

2025

Lysophosphatidylcholine promoting α-Synuclein aggregation in Parkinson's disease: disrupting GCase glycosylation and lysosomal α-Synuclein degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Chunyan Mu,Kaiquan Shao,Mingyu Su,Yurong Guo,Yuxiang Qiu,Ruiao Sun,Sihan Sun,Yaoyu Sun,Chenkai Liu,Wei Wang,Xiaoling Qin,Chuanxi Tang

Cell biology and toxicology 41:34 PubMed39847159

2025

Manganese exposure induces parkinsonism-like symptoms by Serpina3n-TFEB-v/p-ATPase signaling mediated lysosomal dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Huihui Hong,Sicheng Liu,Ting Yang,Jinxian Lin,Kun Luo,Yudong Xu,Ting Li,Yu Xi,Lingling Yang,Yuan-Qiang Lu,Wei Yuan,Zhou Zhou

CNS neuroscience & therapeutics 30:e70088 PubMed39444113

2024

Microglial Melatonin Receptor 1 Degrades Pathological Alpha-Synuclein Through Activating LC3-Associated Phagocytosis In Vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Yu Yao,Bing-Er Cao,Jun-Yi Liu,Qian-Kun Lv,Jia-Rui Zhang,Xiao-Yu Cheng,Cheng-Jie Mao,Quan-Hong Ma,Fen Wang,Chun-Feng Liu

NPJ Parkinson's disease 10:194 PubMed39443520

2024

CRBN modulates synuclein fibrillation via degradation of DNAJB1 in mouse model of Parkinson disease.

Applications

Unspecified application

Species

Unspecified reactive species

Uroos Akber,Jun-Hyung Jung,Heewoong Yoon,Jiwon Seo,Chul-Seung Park

International journal of medical sciences 21:1472-1490 PubMed38903914

2024

Retinal ganglion cell type-specific expression of synuclein family members revealed by scRNA-sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Qingwen Yang,Lin Liu,Fang He,Wenna Zhao,Zhongqun Chen,Xiaotian Wu,Bilin Rao,Xin Lin,Fangyuan Mao,Jia Qu,Jun Zhang

Neuropathology and applied neurobiology 50:e12973 PubMed38558379

2024

Argyrophilic grain disease and co-pathologies in an older patient with a rapidly progressive neuropsychiatric syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Camilla N Clark,Norman Poole,Jeremy D Isaacs,Andrew D MacKinnon,Philip Rich,Leslie R Bridges,Zane Jaunmuktane,Elizabeth Caruana Galizia

Scientific reports 14:6239 PubMed38486089

2024

Solubility of α-synuclein species in the L62 mouse model of synucleinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Karima Schwab,Mandy Magbagbeolu,Franz Theuring,Charles R Harrington,Claude M Wischik,Gernot Riedel

Journal of pineal research 76:e12948 PubMed38488331

2024

Melatonin MT1 receptors regulate the Sirt1/Nrf2/Ho-1/Gpx4 pathway to prevent α-synuclein-induced ferroptosis in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qian-Kun Lv,Kang-Xin Tao,Xiao-Yu Yao,Meng-Zhu Pang,Bing-Er Cao,Chun-Feng Liu,Fen Wang

View all publications

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Anti-Alpha-synuclein抗体[4D6] - BSA and Azide free