重组Anti-Alpha SNAP抗体[EPR7372] (ab133673)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7372] to Alpha SNAP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Alpha SNAP抗体[EPR7372]
参阅全部 Alpha SNAP 一抗 -
描述
兔单克隆抗体[EPR7372] to Alpha SNAP -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: Flow Cyt or IP -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- HeLa cell lysate, Molt4 cell lysate, HepG2 cells and cell lysate, Human kidney tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 9.30 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7372 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab133673于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Perform antigen retrieval before commencing with IHC staining protocol |
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ICC/IF |
1/100 - 1/250.
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说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform antigen retrieval before commencing with IHC staining protocol |
ICC/IF
1/100 - 1/250. |
靶标
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功能
Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus. -
序列相似性
Belongs to the SNAP family. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 8775 Human
- Entrez Gene: 108124 Mouse
- Entrez Gene: 140673 Rat
- Omim: 603215 Human
- SwissProt: P54920 Human
- SwissProt: Q9DB05 Mouse
- SwissProt: P54921 Rat
- Unigene: 126938 Human
see all -
别名
- Alpha soluble NSF attachment protein antibody
- Alpha-soluble NSF attachment protein antibody
- N ethylmaleimide sensitive factor attachment protein alpha antibody
see all
图片
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All lanes : Anti-Alpha SNAP antibody [EPR7372] (ab133673) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Molt4 cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 33 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha SNAP antibody [EPR7372] (ab133673)
Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Alpha SNAP with ab133673 at 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunofluorescent staining of HepG2 cells labelling Alpha SNAP with ab133673 at 1/100.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab133673 被引用在 2 文献中.
- Huang X et al. Mechanistic insights into the SNARE complex disassembly. Sci Adv 5:eaau8164 (2019). PubMed: 30989110
- Lin Y et al. SNARE-Mediated Cholesterol Movement to Mitochondria Supports Steroidogenesis in Rodent Cells. Mol Endocrinol 30:234-47 (2016). PubMed: 26771535