Anti-alpha smooth muscle Actin抗体(ab21027)
Key features and details
- Goat polyclonal to alpha smooth muscle Actin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-alpha smooth muscle Actin抗体
参阅全部 alpha smooth muscle Actin 一抗 -
描述
山羊多克隆抗体to alpha smooth muscle Actin -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Dog, Pig
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免疫原
Synthetic peptide corresponding to Human alpha smooth muscle Actin aa 1-100 (N terminal).
Database link: P62736 -
阳性对照
- WB: Human and rat duodenum lysate. HeLa and NIH/3T3 whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21027于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (2) |
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
A 1 hour primary incubation at room temperature is recommended for this product. |
| 说明 |
|---|
|
WB
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa). A 1 hour primary incubation at room temperature is recommended for this product. |
靶标
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功能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
疾病相关
Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. -
序列相似性
Belongs to the actin family. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 477587 Dog
- Entrez Gene: 59 Human
- Entrez Gene: 11475 Mouse
- Entrez Gene: 733615 Pig
- Entrez Gene: 81633 Rat
- Omim: 102620 Human
- SwissProt: P62736 Human
- SwissProt: P62737 Mouse
see all -
别名
- a actin antibody
- AAT6 antibody
- ACTA_HUMAN antibody
see all
图片
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Western blot - Anti-alpha smooth muscle Actin antibody (ab21027)All lanes : Anti-alpha smooth muscle Actin antibody (ab21027) at 1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 42 kDaLysates in RIPA buffer.
Detected by chemiluminescence.
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Western blot - Anti-alpha smooth muscle Actin antibody (ab21027)Anti-alpha smooth muscle Actin antibody (ab21027) at 1 µg/ml + Rat Duodenum lysate at 35 µg
Predicted band size: 42 kDaDetected by chemiluminescence.
数据表及文件
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SDS download
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Datasheet download
文献 (148)
ab21027 被引用在 148 文献中.
- Hongo H et al. Somatic GJA4 gain-of-function mutation in orbital cavernous venous malformations. Angiogenesis 26:37-52 (2023). PubMed: 35902510
- Zeng W et al. CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype. Oncogene 42:224-237 (2023). PubMed: 36418470
- Dudakovic A et al. The epigenetic regulator BRD4 is required for myofibroblast differentiation of knee fibroblasts. J Cell Biochem 124:320-334 (2023). PubMed: 36648754
- Zhang R et al. Rate of castration-induced prostate stroma regression is reduced in a mouse model of benign prostatic hyperplasia. Am J Clin Exp Urol 11:12-26 (2023). PubMed: 36923722
- Patzek S et al. Loss of Fgf9 in mice leads to pancreatic hypoplasia and asplenia. iScience 26:106500 (2023). PubMed: 37096042