AB21027

Anti-alpha smooth muscle Actin 抗体

Name: alpha smooth muscle Actin抗体 (ab21027)| Abcam中文官网
Brand: Abcam Limited
SKU: AB21027
Price: 5100 CNY
Availability: InStock
Rating: 4 (23 reviews)

Anti-alpha smooth muscle Actin antibody

(23 Reviews)

(183 Publications)

Anti-alpha smooth muscle Actin antibody (ab21027) is a goat polyclonal antibody detecting alpha smooth muscle Actin in Western Blot. Suitable for Human, Mouse, Rat.

- Over 140 publications
- Trusted since 2005

查看别名

ACTSA, ACTVS, GIG46, ACTA2, Alpha-actin-2, Cell growth-inhibiting gene 46 protein

2 Images

Supplier Data

Western blot - Anti-alpha smooth muscle Actin antibody (AB21027)

Lysates in RIPA buffer.

Detected by chemiluminescence.

All lanes:

Western blot - Anti-alpha smooth muscle Actin antibody (ab21027) at 1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 35 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 35 µg

Predicted band size: 42 kDa

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Supplier Data

Western blot - Anti-alpha smooth muscle Actin antibody (AB21027)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-alpha smooth muscle Actin antibody (ab21027) at 1 µg/mL

All lanes:

Rat Duodenum lysate at 35 µg

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关键信息

宿主种属

Goat

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

applications

免疫原

Synthetic Peptide within Human ACTA2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P62736

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "A 1 hour primary incubation at room temperature is recommended for this product." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "A 1 hour primary incubation at room temperature is recommended for this product." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-1 µg/mL", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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产品详情

What is this antibody validated in?
Anti-alpha smooth muscle Actin antibody (ab21027) is a goat polyclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse, Rat samples.

What is the molecular weight of alpha smooth muscle Actin?
Anti-alpha smooth muscle Actin (ab21027) specifically detects a band for alpha smooth muscle Actin (UniProt: P62736) at a molecular weight of 42kDa.

Trusted by the scientific community
Anti-alpha smooth muscle Actin (ab21027) was first used in a scientific publication in 2005 and has been cited over 140 times in peer-reviewed journals.

Reviewed by scientists
Anti-alpha smooth muscle Actin (ab21027) has over 20 independent reviews from customers.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

存储溶液

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Alpha smooth muscle actin (α-SMA) also known as ACTA2 is an actin isoform with a specific role in the contractile function of smooth muscle cells. The molecular weight of α-SMA is approximately 42 kDa. This protein is expressed widely in vascular smooth muscle cells the peritoneal lining and myofibroblasts. It often serves as a marker for these cell types. The expression of α-SMA is critical for the mechanical attributes of cells contributing to the rigidity and contractility of tissues where it is present.

Biological function summary

Alpha smooth muscle actin aids in maintaining the structural integrity of tissues by forming part of the actin cytoskeleton an important element in cellular support. It exists in high concentration in stress fibers contributing to cellular movements and shape maintenance. These actions are essential in various dynamic cellular processes such as cell migration and adhesion. Alpha smooth muscle actin does not typically form complexes but it associates with other components of the actin cytoskeleton to ensure cell stability and function.

Pathways

Alpha smooth muscle actin functions significantly within the TGF-beta signaling pathway which influences cell proliferation differentiation and apoptosis. It interacts closely with proteins such as myosin to facilitate cellular contractility and motility. Additionally α-SMA plays a part in the RhoA/Rho kinase (ROCK) pathway connecting with regulators of actin filament organization. These pathways are essential for modulation of smooth muscle contraction and actin filament assembly contributing to vascular development and wound healing.

Alpha smooth muscle actin is importantly involved in fibrotic diseases and vascular diseases like arteriosclerosis. Increased expression of α-SMA is often observed in fibrotic tissues contributing to pathogenesis due to excessive extracellular matrix deposition. In the context of vascular disorders overexpression of α-SMA can lead to abnormal vascular remodeling often seen in diseases like hypertension. Interactions with proteins such as fibronectin and collagens facilitate these pathological changes highlighting the importance of α-SMA in disease development.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

See full target information ACTA2

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文献 (183)

Recent publications for all applications. Explore the full list and refine your search

Stem cells translational medicine 14: PubMed40973918

2025

Rapid manufacturing of angiogenic cellular collagen patches for ischemic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Pfrender,Sungwoo Kim,John A Farag,Shin Yajima,Yujiro Kawai,Koji Kawago,Umayr Syed,Gentaro Ikeda,Tsuyoshi Ueyama,Hiroyuki Takashima,Alex Dalal,Yuanjia Zhu,Kenzo Ichimura,Yu Liu,Seyedsina Moeinzadeh,Jayme Koltsov,Joseph C Wu,Y Joseph Woo,Phillip C Yang,Yunzhi P Yang,Yasuhiro Shudo

Nature communications 16:8295 PubMed40957887

2025

A mesothelial differentiation gateway drives fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Safwen Kadri,Adrian Fischer,Martin Mück-Häusl,Wei Han,Amal Kadri,Yue Lin,Lin Yang,Shaoping Hu,Haifeng Ye,Pushkar Ramesh,Meshal Ansari,Herbert B Schiller,Hans-Günther Machens,Yuval Rinkevich

iScience 28:112885 PubMed40678517

2025

Gpr37 modulates the severity of inflammation-induced GI dysmotility by regulating enteric reactive gliosis.

Applications

Unspecified application

Species

Unspecified reactive species

Keiramarie Robertson,Oliver Hahn,Adarsh Tantry,Beatriz G Robinson,Arwa T Faruk,Mathangi Janakiraman,Hong Namkoong,Kwangkon Kim,Jiayu Ye,Estelle Spear Bishop,Randy A Hall,Tony Wyss-Coray,Laren Becker,Julia A Kaltschmidt

NPJ systems biology and applications 11:67 PubMed40593928

2025

Temporal analysis of doxorubicin-induced cardiac toxicity and hypertrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Te Lin,Yi-Ju Lee,Wen-Wei Tseng,Zih-Hua Chen,Huai-Ching Hsieh,Ko-Hong Lin,Jin-Yu Su,An-Chi Wei

Future science OA 11:2483147 PubMed40155367

2025

Hesperidin alleviates pulmonary fibrosis by regulating EI24-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Zhang,Wen Ma,Fang Wan

PloS one 20:e0316764 PubMed39970160

2025

Sodium propionate decreases implant-induced foreign body response in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Deivenita Juliana Alves Carvalho do Carmo,Marcela Guimarães Takahashi Lazari,Letícia Cristine Cardoso Dos Santos,Pedro Augusto Carvalho Costa,Itamar Couto Guedes Jesus,Silvia Guatimosim,Pedro Pires Goulart Guimaraes,Silvia Passos Andrade,Paula Peixoto Campos

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2409250 PubMed39716891

2024

TTK Inhibition Alleviates Postinjury Neointimal Formation and Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie-Hong Wu,Yu-Xiao Liu,Jia-Bin Zong,Min Qiu,Yi-Fan Zhou,Ya-Nan Li,Tuersun Aili,Xin-Ran Zhao,Bo Hu

Journal of cellular and molecular medicine 28:e70141 PubMed39482983

2024

NAT10-mediated RNA ac4C acetylation contributes to the myocardial infarction-induced cardiac fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Li,Feierkaiti Yushanjiang,Zhao Fang,Wan-Li Liu

American journal of physiology. Lung cellular and molecular physiology 328:L290-L300 PubMed39437757

2024

Aging, brain-derived neurotrophic factor, and allergen-induced pulmonary responses in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Li Y Drake,Benjamin B Roos,Sarah A Wicher,Latifa Khalfaoui,Lisa L Nesbitt,Yun Hua Fang,Christina M Pabelick,Y S Prakash

Histochemistry and cell biology 162:523-539 PubMed39317805

2024

Expression of mRNA for molecules that regulate angiogenesis, endothelial cell survival, and vascular permeability is altered in endothelial cells isolated from db/db mouse hearts.

Applications

Unspecified application

Species

Unspecified reactive species

Krzysztof Bartkowiak,Mateusz Bartkowiak,Ewa Jankowska-Steifer,Anna Ratajska,Elżbieta Czarnowska,Marek Kujawa,Olga Aniołek,Justyna Niderla-Bielińska

View all publications

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Anti-alpha smooth muscle Actin 抗体

Anti-alpha smooth muscle Actin antibody

Western blot - Anti-alpha smooth muscle Actin antibody (AB21027)

Western blot - Anti-alpha smooth muscle Actin antibody (AB21027)