Anti-Alpha Skeletal Muscle Actin抗体[Alpha Sr-1] (ab28052)
![Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)](https://www.abcam.cn/ps/products/28/ab28052/Images/ab28052-415198-anti-alpha-skeletal-muscle-actin-antibody-immunocytochemistry-hela-human.jpg "Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)")
Key features and details
- Mouse monoclonal [Alpha Sr-1] to Alpha Skeletal Muscle Actin
- Suitable for: WB, ICC
- Reacts with: Rat, Human
- Isotype: IgM
概述
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产品名称
Anti-Alpha Skeletal Muscle Actin抗体[Alpha Sr-1]
参阅全部 Alpha Skeletal Muscle Actin 一抗 -
描述
小鼠单克隆抗体[Alpha Sr-1] to Alpha Skeletal Muscle Actin -
宿主
Mouse -
特异性
In Western blotting of pure rabbit striated muscle actin or of total extracts from different tissues, e.g. rat striated muscle, rat myocardium, rat aortic media, human fibroblasts and pure chicken gizzard actin, ab28052 labels alpha-skeletal (alpha-sarcomeric) and alpha-cardiac muscle actins. No labelling is observed with actin from fibroblasts (alpha-smooth muscle and beta-cytoplasmic actin), aorta (alpha-smooth muscle actin), or chicken gizzard (gamma-smooth muscle actin). -
经测试应用
适用于: WB, ICCmore details -
种属反应性
与反应: Rat, Human -
免疫原
Purified striated muscle actin from rabbit.
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阳性对照
- WB: Whole extract of Rat heart ICC: HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.097% Sodium azide
Constituent: 0.79% Tris HCl -
Concentration information loading... -
纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
Alpha Sr-1 -
同种型
IgM -
研究领域
相关产品
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Compatible Secondaries
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28052于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa.
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| ICC |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa. |
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ICC
Use at an assay dependent concentration. |
靶标
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功能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
疾病相关
Defects in ACTA1 are the cause of nemaline myopathy type 3 (NEM3) [MIM:161800]. A form of nemaline myopathy. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. The phenotype at histological level is variable. Some patients present areas devoid of oxidative activity containg (cores) within myofibers. Core lesions are unstructured and poorly circumscribed.
Defects in ACTA1 are a cause of myopathy congenital with excess of thin myofilaments (MPCETM) [MIM:161800]. A congenital muscular disorder characterized at histological level by areas of sarcoplasm devoid of normal myofibrils and mitochondria, and replaced with dense masses of thin filaments. Central cores, rods, ragged red fibers, and necrosis are absent.
Defects in ACTA1 are a cause of congenital myopathy with fiber-type disproportion (CFTD) [MIM:255310]; also known as congenital fiber-type disproportion myopathy (CFTDM). CFTD is a genetically heterogeneous disorder in which there is relative hypotrophy of type 1 muscle fibers compared to type 2 fibers on skeletal muscle biopsy. However, these findings are not specific and can be found in many different myopathic and neuropathic conditions. -
序列相似性
Belongs to the actin family. -
细胞定位
Cytoplasm > cytoskeleton. - Information by UniProt
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数据库链接
- Entrez Gene: 58 Human
- Entrez Gene: 29437 Rat
- Omim: 102610 Human
- SwissProt: P68133 Human
- SwissProt: P68136 Rat
- Unigene: 1288 Human
- Unigene: 82732 Rat
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别名
- a actin antibody
- ACTA antibody
- ACTA1 antibody
see all
图片
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Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)Immunocytochemistry analysis of HeLa cells labeling Alpha Skeletal Muscle Actin with ab28052 at 1/1000. Cells were fixed and permeabilized with methanol followed by aceton. The antibody was developed using Goat Anti-Mouse IgG, Cy3™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
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![Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)](https://www.abcam.cn/ps/products/28/ab28052/Images/ab28052-415199-anti-alpha-skeletal-muscle-actin-antibody-western-blot-heart-rat.jpg)
Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)Lane 1 : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/500 dilution
Lane 2 : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/1000 dilution
Lane 3 : Mouse Monoclonal Anti-Actin (a-Sarcomeric) antibody at 1/500 dilution
All lanes : Whole extract of Rat heart
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase
Predicted band size: 42 kDachemiluminescent substrate.
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![Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)](https://www.abcam.cn/ps/products/28/ab28052/Images/ab28052-38957-anti-alpha-skeletal-muscle-actin-antibody-alpha-sr-1-immunofluorescence.jpg)
Immunocytochemistry - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)This image is courtesy of an Abreview submitted by Richard Patten.ab28052 at dilution 1/200 staining alpha sarcomeric actin from rat neonatal cardiac myocytes by immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% NP40 and incubated with the antibody for 45 minutes. A Cy3® donkey anti-mouse antibody was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.
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![Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)](https://www.abcam.cn/ps/products/28/ab28052/Images/ab28052-38956-anti-alpha-skeletal-muscle-actin-antibody-alpha-sr-1-western-blot.jpg)
Western blot - Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052)This image is courtesy of an Abreview submitted by Richard Patten.All lanes : Anti-Alpha Skeletal Muscle Actin antibody [Alpha Sr-1] (ab28052) at 1/200 dilution
All lanes : 40 µg rat neonatal cardiac myocytes (whole cell lysate)
Secondary
All lanes : HRP polymer conjugated donkey anti-mouse IgG at 1/1000
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 1 second
This image is courtesy of an Abreview submitted by Dr. Richard Patten on 12 May 2008.
数据表及文件
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SDS download
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Datasheet download
文献 (50)
ab28052 被引用在 50 文献中.
- Chen H et al. IFNγ Transcribed by IRF1 in CD4+ Effector Memory T Cells Promotes Senescence-Associated Pulmonary Fibrosis. Aging Dis 14:2215-2237 (2023). PubMed: 37199578
- O'Brien E et al. Pharmacological EZH2 inhibition combined with retinoic acid treatment promotes differentiation and apoptosis in rhabdomyosarcoma cells. Clin Epigenetics 15:167 (2023). PubMed: 37858275
- Huang M et al. Regulation of Myogenesis by a Na/K-ATPase α1 Caveolin-Binding Motif. Stem Cells 40:133-148 (2022). PubMed: 35257186
- Wsol A et al. Increased oxytocinergic system activity in the cardiac muscle in spontaneously hypertensive SHR rats. Arch Med Sci 18:1088-1094 (2022). PubMed: 35832696
- Zhou J et al. Sirt1 overexpression improves senescence-associated pulmonary fibrosis induced by vitamin D deficiency through downregulating IL-11 transcription. Aging Cell 21:e13680 (2022). PubMed: 35906886