为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 定制化产品 & 合作关系
    定制化产品 & 合作关系

    定制化产品和商务合作加速您的诊断和治疗项目

    定制化产品

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

Neuroscience Cell Adhesion Proteins Cytoskeletal Proteins Intermediate Filaments

Anti-alpha Internexin抗体(ab7259)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (7)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

运输费用

Promotion Information

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-alpha Internexin antibody (ab7259)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-alpha Internexin antibody (ab7259)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody (ab7259)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody (ab7259)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody (ab7259)

Key features and details

  • Rabbit polyclonal to alpha Internexin
  • Suitable for: WB, IHC-FoFr, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG

你可能会感兴趣

Western blot
Product image
InstantBlue® Coomassie Protein Stain (ISB1L) (ab119211)
蛋白
Product image
Recombinant Human alpha Internexin protein (ab160346)
二抗
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

查看更多相关产品

概述

  • 产品名称

    Anti-alpha Internexin抗体
    参阅全部 alpha Internexin 一抗
  • 描述

    兔多克隆抗体to alpha Internexin
  • 宿主

    Rabbit
  • 特异性

    Specifically recognizes a-internexin.
  • 经测试应用

    适用于: WB, IHC-FoFr, IHC-P, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat, Cow, Human
    预测可用于: Mammals
  • 免疫原

    Recombinant full length protein (Rat)

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.60
    Preservatives: 0.01% Thimerosal (merthiolate), 0.05% Sodium azide
    Constituents: 0.164% Sodium phosphate, 1.45% Sodium chloride, 1.5% BSA
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Cell Adhesion Proteins
    • Cytoskeletal Proteins
    • Intermediate Filaments
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Neuroscience
    • Development

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human alpha Internexin protein (ab160346)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7259于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/10000 - 1/20000. Predicted molecular weight: 66 kDa.
IHC-FoFr
1/500 - 1/1000.
IHC-P
1/2000.
ICC/IF
1/500 - 1/1000.
说明
WB
1/10000 - 1/20000. Predicted molecular weight: 66 kDa.
IHC-FoFr
1/500 - 1/1000.
IHC-P
1/2000.
ICC/IF
1/500 - 1/1000.

靶标

  • 功能

    Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
  • 组织特异性

    Found predominantly in adult CNS.
  • 序列相似性

    Belongs to the intermediate filament family.
  • 发展阶段

    Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.
  • 翻译后修饰

    O-glycosylated.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Target information above from: UniProt accession Q16352 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 9118 Human
    • Entrez Gene: 226180 Mouse
    • Entrez Gene: 24503 Rat
    • Omim: 605338 Human
    • SwissProt: Q16352 Human
    • SwissProt: P46660 Mouse
    • SwissProt: P23565 Rat
    • Unigene: 500916 Human
    • Unigene: 276251 Mouse
    • Unigene: 88941 Rat
    see all
  • 别名

    • 66 kDa neurofilament protein antibody
    • AINX_HUMAN antibody
    • Alpha Inx antibody
    • Alpha-internexin antibody
    • Alpha-Inx antibody
    • INA antibody
    • Internexin neuronal intermediate filament protein alpha antibody
    • MGC12702 antibody
    • NEF 5 antibody
    • NEF5 antibody
    • Neurofilament 5 (66kD) antibody
    • Neurofilament 5 antibody
    • Neurofilament 66 antibody
    • Neurofilament 66 tax binding protein antibody
    • Neurofilament-66 antibody
    • NF 66 antibody
    • NF-66 antibody
    • NF66 antibody
    • TXBP 1 antibody
    • TXBP1 antibody
    see all

图片

  • Western blot - Anti-alpha Internexin antibody (ab7259)
    Western blot - Anti-alpha Internexin antibody (ab7259)
    All lanes : Anti-alpha Internexin antibody (ab7259) at 1/10000 dilution

    Lane 1 : Protein standard
    Lane 2 : Mouse spinal cord lysate
    Lane 3 : Rat spinal cord lysate
    Lane 4 : Cow spinal cord lysate

    Predicted band size: 66 kDa

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-alpha Internexin antibody (ab7259)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-alpha Internexin antibody (ab7259)

    Immunofluorescence analysis of rat cerebellum section stained for alpha Internexin using ab7259 at a 1/2000 dilution (green) co-strained with chicken polyclonal antibody to GFAP at a 1/5000 dilution (red). DAPI was used to stain nuclear DNA (blue).

    Following transcardial perfusion with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 μM, and free-floating sections were stained.

  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody (ab7259)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody (ab7259)

    Ab7259 shows red, neuronal progenitor cells, plectin (not one of our antibodies) shows fibroblast marker. Photo courtesy of: Dr. Gerry Shaw University of Florida

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody (ab7259)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Internexin antibody (ab7259)
    Ab7259 staining Human normal temporal cortex. Staining is localised to intracellualr compartment.
    Left panel: with primary antibody diluted 1:2000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody (ab7259)
    Immunocytochemistry/ Immunofluorescence - Anti-alpha Internexin antibody (ab7259)
    ICC/IF image of ab7259 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7259, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (7)

发表研究结果有使用 ab7259?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7259 被引用在 7 文献中.

  • Kullmann JA  et al. Oxygen Tension and the VHL-Hif1a Pathway Determine Onset of Neuronal Polarization and Cerebellar Germinal Zone Exit. Neuron 106:607-623.e5 (2020). PubMed: 32183943
  • Song S  et al. Postnatal accumulation of intermediate filaments in the cat and human primary visual cortex. J Comp Neurol 523:2111-26 (2015). PubMed: 25823892
  • Nakamura M  et al. A truncating SOD1 mutation, p.Gly141X, is associated with clinical and pathologic heterogeneity, including frontotemporal lobar degeneration. Acta Neuropathol 130:145-57 (2015). PubMed: 25917047
  • Karpova A  et al. Encoding and transducing the synaptic or extrasynaptic origin of NMDA receptor signals to the nucleus. Cell 152:1119-33 (2013). ICC/IF, WB ; Rabbit . PubMed: 23452857
  • Balko JM  et al. Profiling of residual breast cancers after neoadjuvant chemotherapy identifies DUSP4 deficiency as a mechanism of drug resistance. Nat Med 18:1052-9 (2012). WB . PubMed: 22683778
  • Samara A  et al. Proteomics reveal rat hippocampal lateral asymmetry. Hippocampus 21:108-19 (2011). WB ; Rat . PubMed: 20020437
  • Snowden JS  et al. The clinical diagnosis of early-onset dementias: diagnostic accuracy and clinicopathological relationships. Brain 134:2478-92 (2011). PubMed: 21840888

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-3 of 3 Abreviews or Q&A

Western blot abreview for Anti-alpha Internexin antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 05 2013

Immunohistochemistry (Frozen sections) abreview for Anti-alpha Internexin antibody

Poor
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 12 2012

Question

I would like to test the following antibodies in Apteronotus leptorhynchus via IHC-Fr:
ab7654
ab22040
ab7259
ab118982
ab124327
ab40758

Read More

Abcam community

Verified customer

Asked on Mar 21 2012

Answer


I am very pleased to hear you would like to accept our offer and test the above antivbodies in Apteronotus leptorhynchus. This code will give you 1 free primary antibody before the expiration date for each code. To redeem this offer, please submit an Abreview for Apteronotus leptorhynchus and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

回复于 Mar 21 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

通过邮件收到学术资源和促销信息 注册
研究类别(A-Z)
  • 肿瘤研究
  • 心血管研究
  • 细胞生物学
  • 发育生物学
  • 染色质及细胞核信号转导研究
  • 免疫学
  • 代谢
  • 微生物学
  • 神经生物学
  • 信号转导研究
  • 干细胞研究
产品种类(A-Z)
  • 一抗
  • 二抗
  • 生化试剂
  • 亚类对照
  • 流式细胞仪多色选择器
  • 试剂盒
  • 内参对照
  • 细胞裂解液
  • 多肽
  • 蛋白酶
  • 组织切片
  • 蛋白标签及细胞标记物
  • 相关试剂及工具
帮助与支持
  • 技术支持
  • 技术FAQ
  • 实验步骤与建议
  • 购买FAQ
  • RabMAb 产品
  • 培训课程
  • 按靶标浏览
企业
  • 企业资讯
  • 投资者关系
  • 公司新闻
  • 招贤纳士
  • 关于我们
活动通知
  • 商品展览
  • 会议
全球网站
  • abcam.com
  • abcam.co.jp
QR code for Weechat

微信扫码 关注我们

沪ICP备14046373号-1

沪公网安备 31011502007413号

  • 销售条款
  • 网站使用条款
  • Cookie政策
  • 隐私权政策
  • 法律
© 1998-2023 艾博抗(上海)贸易有限公司版权所有