重组Anti-alpha Defensin 5抗体[EPR14309(B)] (ab180515)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14309(B)] to alpha Defensin 5
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-alpha Defensin 5抗体[EPR14309(B)]
参阅全部 alpha Defensin 5 一抗 -
描述
兔单克隆抗体[EPR14309(B)] to alpha Defensin 5 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human alpha Defensin 5 aa 1 to the C-terminus (internal sequence). The exact sequence is proprietary.
Database link: Q01523 -
阳性对照
- Human small intestine - paraffin embedded tissue and tissue lysate.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR14309(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180515于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 10 kDa.
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IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 10 kDa. |
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
靶标
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功能
Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. All DEFA5 peptides exert antimicrobial activities, but their potency is affected by peptide processing. -
组织特异性
Paneth cells of the small intestine (at protein level). -
序列相似性
Belongs to the alpha-defensin family. -
翻译后修饰
Glycosylated. -
细胞定位
Secreted. Cytoplasmic vesicle > secretory vesicle. Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues, HD5(20-94) being the predominant intracellular form. Peptides HD5(56-94) and HD5(63-94) are found in the extracellular milieu, HD5(63-94) being the most abundant form. - Information by UniProt
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数据库链接
- Entrez Gene: 1670 Human
- Omim: 600472 Human
- SwissProt: Q01523 Human
- Unigene: 655233 Human
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别名
- alpha 5 antibody
- DEF5 antibody
- DEF5_HUMAN antibody
see all
图片
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Anti-alpha Defensin 5 antibody [EPR14309(B)] (ab180515) at 1/10000 dilution + Human small intestine lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 10 kDa -
Immunohistochemical analysis of paraffin-embedded Human small intestine tissue labeling alpha Defensin 5 with ab180515 at 1/4000 dilution. Anti-rabbit HRP secondary antibody was used and counter staining with Hematoxylin performed.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (2)
ab180515 被引用在 2 文献中.
- Capeling MM et al. Suspension culture promotes serosal mesothelial development in human intestinal organoids. Cell Rep 38:110379 (2022). PubMed: 35172130
- Wu Z et al. The inhibitory effect of human DEFA5 in growth of gastric cancer by targeting BMI1. Cancer Sci 112:1075-1083 (2021). PubMed: 33503272