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AB192919

Anti-alpha COP I/COPA抗体- C-terminal

Anti-alpha COP I/COPA antibody - C-terminal

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(2 Publications)

Rabbit Polyclonal alpha COP I/COPA antibody. C-terminal. Suitable for WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Coatomer subunit alpha aa 1150 to C-terminus.

查看别名

Coatomer subunit alpha, Alpha-coat protein, HEP-COP, Alpha-COP, HEPCOP, COPA

1 Images
Western blot - Anti-alpha COP I/COPA antibody - C-terminal (AB192919)
  • WB

Supplier Data

Western blot - Anti-alpha COP I/COPA antibody - C-terminal (AB192919)

All lanes:

Western blot - Anti-alpha COP I/COPA antibody - C-terminal (ab192919) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 138 kDa

true

Exposure time: 3min

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

WB

applications

免疫原

Synthetic Peptide within Human Coatomer subunit alpha aa 1150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P53621

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替代产品

Primary Antibodies

AB181224

Anti-alpha COP I/COPA antibody [EPR14273(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha COP I/COPA antibody [EPR14273(B)] (AB181224)

  • 0
  • 0
Primary Antibodies

AB2913

Anti-alpha COP I/COPA antibody

Immunocytochemistry/ Immunofluorescence - Anti-alpha COP I/COPA antibody (AB2913)

  • 0
  • 0

搭配使用产品

Primary Antibodies

AB2899

Anti-beta COP antibody

Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (AB2899)

  • 5
  • 15
Primary Antibodies

AB21679

Anti-Clathrin heavy chain antibody

Western blot - Anti-Clathrin heavy chain antibody (AB21679)

  • 5
  • 38
Primary Antibodies

AB192924

Anti-COPB2 antibody - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPB2 antibody - C-terminal (AB192924)

  • 0
  • 0
Reagents, Controls & Accessories

AB176753

Phalloidin-iFluor 488 Reagent

Immunocytochemistry/ Immunofluorescence - Phalloidin-iFluor 488 Reagent (AB176753)

  • 5
  • 14
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Immunogen
纯化说明
ab192919 was affinity purified using an epitope specific to alpha COP I/COPA immobilized on solid support.
存储溶液
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Alpha COP I also called COPA protein or alpha COPS is a component of the COPI coatomer complex involved in intracellular transport. This protein weighs approximately 134 kDa. Alpha COP is mainly expressed in the Golgi apparatus and other intracellular organelles. It functions as part of the COPI complex which is essential for retrograde Golgi-to-ER transport of proteins and lipids. This complex also plays an important role in maintaining Golgi structure and functionality.
Biological function summary

The alpha COP participates in forming the cytosolic coatomer complex which contains seven different proteins. This complex is involved in the transport of vesicles within eukaryotic cells. Alpha COP helps in the recruitment and assembly of other coatomer subunits necessary for vesicle budding and cargo selection. The protein interacts with other vesicle-forming machinery components ensuring efficient segregation and transport of cargo molecules. Additionally COPA notes contribute to cell homeostasis through effectively gathering sets of transport proteins.

Pathways

Alpha COP influences the regulation of the secretory pathway and lipid biosynthesis. The coatomer proteins including alpha COP interact with Arf family proteins which are critical regulators of vesicle formation by promoting membrane curvature and binding cargo. Another pathway involving alpha COP is the endocytosis pathway which is important for the recycling of plasma membrane components. This pathway also connects alpha COP with other proteins related to clathrin-mediated vesicle trafficking.

Defects in alpha COP have links to hereditary disease known as COPA syndrome which manifests with autoimmune features and interstitial lung disease. Mutations in the COPA gene impair protein transport function leading to improper immune responses. Alpha COP's interaction with proteins in the autoimmunity pathways can further relate it to rheumatoid arthritis. It affects the immune system's regulatory mechanisms and shares interaction partners with other immune system proteins such as cytokines and transcription factors involved in inflammatory processes.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).. Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor.
See full target information Coatomer subunit alpha
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:402 PubMed37414755

2023

USP11-mediated LSH deubiquitination inhibits ferroptosis in colorectal cancer through epigenetic activation of CYP24A1.

Applications

Unspecified application

Species

Unspecified reactive species

Junyi Duan,Daoyuan Huang,Cheng Liu,Yangbo Lv,Lei Zhang,Fen Chang,Xiangyu Zeng,Li Li,Weiping Wang,Genze Shao

Journal of virology 96:e0219321 PubMed35044210

2022

ARF1 with Sec7 Domain-Dependent GBF1 Activates Coatomer Protein I To Support Classical Swine Fever Virus Entry.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhang,Tao Wang,Yanyan Yi,Mengzhao Song,Mingxing Jin,Kangkang Guo,Yanming Zhang
View all publications
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