AB5577

Anti-Alpha B Crystallin (phospho S59)抗体

Name: Alpha B Crystallin(磷酸化S59)抗体 (ab5577)| Abcam中文官网
Brand: Abcam Limited
SKU: AB5577
Price: 4988 CNY
Availability: InStock
Rating: 5 (3 reviews)

Anti-Alpha B Crystallin (phospho S59) antibody

(3 Reviews)

(13 Publications)

Rabbit Polyclonal Alpha B Crystallin phospho S59 antibody. Suitable for WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human CRYAB phospho S59 aa 50-100.

查看别名

CRYA2, HSPB5, CRYAB, Alpha-crystallin B chain, Alpha(B)-crystallin, Heat shock protein beta-5, Heat shock protein family B member 5, Renal carcinoma antigen NY-REN-27, Rosenthal fiber component, HspB5

1 Images

Unknown

Western blot - Anti-Alpha B Crystallin (phospho S59) antibody (AB5577)

ab5577 at 0.5μg/ml concentration staining approximately 20 KDa Crystallin Alpha B in lane 1) phospho-alpha-B crystallin (Ser59) and lane 2) non phosphorylated alpha-B crystallin in U-251 MG (formally U-373 MG) (Human brain glioma cell line) whole cell lysate.

All lanes:

Western blot - Anti-Alpha B Crystallin (phospho S59) antibody (ab5577)

Predicted band size: 20 kDa

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关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

Synthetic Peptide within Human CRYAB phospho S59 aa 50-100. The exact immunogen used to generate this antibody is proprietary information.

P02511

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反应性数据

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

存储溶液

Preservative: 0.05% Sodium azide Constituents: PBS, 3% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Alpha B Crystallin also known as CRYAB or alpha B crystallin protein is a small heat shock protein with a molecular mass of approximately 20 kDa. It is expressed in various tissues including the eye lens heart skeletal muscles and the brain. Alpha B Crystallin functions mechanically as a chaperone helping to prevent the aggregation of unfolded proteins by stabilizing them. This protein plays an important role in maintaining proper cellular function especially under stress conditions.

Biological function summary

Alpha B crystallin performs as part of the larger small heat shock protein family which contributes to the cellular defense system. It acts primarily as a chaperone binding to denatured proteins to protect the cells from damage during environmental stresses such as heat and oxidative stress. Alpha B crystallin often forms oligomeric complexes which increases its protective properties. This protein exhibits significant anti-apoptotic functions by interacting with various apoptosis-regulating factors contributing to cellular survival.

Pathways

Alpha B crystallin integrates into the cellular stress response and apoptosis pathways. It involves the PI3K/Akt signaling pathway known for regulating cell survival and preventing programmed cell death. Alpha B crystallin also interacts with other proteins such as Bcl-2 further enhancing its role in anti-apoptotic activities. Its contribution to these pathways highlights its protective function during cellular stress situations.

Alpha B crystallin is associated with cataracts and dilated cardiomyopathy. It plays a significant role in maintaining lens transparency and mutations or dysfunctions in alpha B crystallin can lead to cataract development. Similarly in cardiac tissue abnormal alpha B crystallin expression links to dilated cardiomyopathy. The protein is also known to associate with other structural proteins like desmin where disruptions may contribute to muscle-related diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome (By similarity).

See full target information CRYAB phospho S59

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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed39932799

2025

Phosphorylation of CRYAB induces a condensatopathy to worsen post-myocardial infarction left ventricular remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Moydul Islam,David R Rawnsley,Xiucui Ma,Walter Navid,Chen Zhao,Xumin Guan,Layla Foroughi,John T Murphy,Honora Navid,Carla J Weinheimer,Attila Kovacs,Jessica Nigro,Aaradhya Diwan,Ryan P Chang,Minu Kumari,Martin E Young,Babak Razani,Kenneth B Margulies,Mahmoud Abdellatif,Simon Sedej,Ali Javaheri,Douglas F Covey,Kartik Mani,Abhinav Diwan

iScience 27:109510 PubMed38660406

2024

The activation of LBH-CRYAB signaling promotes cardiac protection against I/R injury by inhibiting apoptosis and ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Anbiao Wu,Chongbin Zhong,Xudong Song,Wen Yuan,Mintian Tang,Tao Shu,Houda Huang,Pingzhen Yang,Qicai Liu

International journal of molecular sciences 23: PubMed36232565

2022

Polo-Like Kinase 2 Plays an Essential Role in Cytoprotection against MG132-Induced Proteasome Inhibition via Phosphorylation of Serine 19 in HSPB5.

Applications

Unspecified application

Species

Unspecified reactive species

Shuji Ueda,Moeka Nishihara,Yuuki Hioka,Ken-Ichi Yoshino,Soichiro Yamada,Minoru Yamanoue,Yasuhito Shirai

Antioxidants (Basel, Switzerland) 9: PubMed32731533

2020

SkQ1 Suppresses the p38 MAPK Signaling Pathway Involved in Alzheimer's Disease-Like Pathology in OXYS Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia A Muraleva,Natalia A Stefanova,Nataliya G Kolosova

Redox biology 24:101183 PubMed30974319

2019

The early response of αB-crystallin to a single bout of aerobic exercise in mouse skeletal muscles depends upon fiber oxidative features.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan Dimauro,Ambra Antonioni,Neri Mercatelli,Elisa Grazioli,Cristina Fantini,Rosario Barone,Filippo Macaluso,Valentina Di Felice,Daniela Caporossi

Laboratory investigation; a journal of technical m 99:1714-1727 PubMed30683901

2019

Lens-specific conditional knockout of Msx2 in mice leads to ocular anterior segment dysgenesis via activation of a calcium signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wenting Yu,Ziyan Yu,Danhong Wu,Jiao Zhang,Ying Zhu,Yang Zhang,Hong Ning,Mingwu Wang,Jinsong Zhang,Jiangyue Zhao

Frontiers in physiology 9:505 PubMed29867551

2018

BYD Ameliorates Oxidative Stress-Induced Myocardial Apoptosis in Heart Failure Post-Acute Myocardial Infarction via the P38 MAPK-CRYAB Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zhang,Chun Li,Hui Meng,Dongqing Guo,Qian Zhang,Wenji Lu,Qixin Wang,Yong Wang,Pengfei Tu

Journal of molecular and cellular cardiology 111:61-68 PubMed28826663

2017

Exercise-responsive phosphoproteins in the heart.

Applications

Unspecified application

Species

Unspecified reactive species

Hongbo Guo,Ruth Isserlin,Andrew Emili,Jatin G Burniston

Proceedings of the National Academy of Sciences of 114:E1745-E1754 PubMed28196893

2017

Phosphorylation of αB-crystallin supports reactive astrogliosis in demyelination.

Applications

Unspecified application

Species

Unspecified reactive species

Hedwich F Kuipers,Jane Yoon,Jack van Horssen,May H Han,Paul L Bollyky,Theo D Palmer,Lawrence Steinman

Proteomics 15:508-519 PubMed25332170

2014

Quantitative phosphoproteomics of Alzheimer's disease reveals cross-talk between kinases and small heat shock proteins.

Applications

Species

Human

Eric B Dammer,Andrew K Lee,Duc M Duong,Marla Gearing,James J Lah,Allan I Levey,Nicholas T Seyfried

View all publications

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Anti-Alpha B Crystallin (phospho S59)抗体

Anti-Alpha B Crystallin (phospho S59) antibody

Western blot - Anti-Alpha B Crystallin (phospho S59) antibody (AB5577)