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Anti-Alpha B Crystallin抗体[1A7.D5] (ab74441)

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Western blot - Anti-Alpha B Crystallin antibody [1A7.D5] (ab74441)
  • Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [1A7.D5] (ab74441)

Key features and details

  • Mouse monoclonal [1A7.D5] to Alpha B Crystallin
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-Alpha B Crystallin抗体[1A7.D5]
    参阅全部 Alpha B Crystallin 一抗

  • 描述

    小鼠单克隆抗体[1A7.D5] to Alpha B Crystallin

  • 宿主

    Mouse

  • 特异性

    Does not cross-react with αA-crystallin, βL-crystallin, βH-crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

  • 经测试应用

    适用于: WB, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Full length native protein (purified) corresponding to Human Alpha B Crystallin.

  • 阳性对照

    • WB: AlphaB crystallin protein. ICC/IF: SK-N-BE cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab74441于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/2000. Predicted molecular weight: 20 kDa.
ICC/IF
1/100.
说明
WB
1/2000. Predicted molecular weight: 20 kDa.
ICC/IF
1/100.

靶标

  • 功能

    May contribute to the transparency and refractive index of the lens.

  • 组织特异性

    Lens as well as other tissues.

  • 疾病相关

    Defects in CRYAB are the cause of myofibrillar alpha-B crystallin-related (MFM-CRYAB) [MIM:608810]. A neuromuscular disorder that results in weakness of the proximal and distal limb muscles, weakness of the neck, velopharynx and trunk muscles, hypetrophic cardiomyopathy, and cataract in a subset of patients.

  • 序列相似性

    Belongs to the small heat shock protein (HSP20) family.

  • 细胞定位

    Cytoplasm. Nucleus. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles.
  • Information by UniProt

  • 数据库链接

    • 别名

      • AACRYA antibody
      • Alpha B crystallin antibody
      • Alpha crystallin B chain antibody
      • Alpha(B)-crystallin antibody
      • Alpha-crystallin B chain antibody
      • CRYA2 antibody
      • Cryab antibody
      • CRYAB_HUMAN antibody
      • Crystallin alpha B antibody
      • Crystallin alpha polypeptide 2 antibody
      • CTPP2 antibody
      • Heat shock 20 kD like protein antibody
      • Heat shock protein beta 5 antibody
      • Heat shock protein beta-5 antibody
      • HspB5 antibody
      • Renal carcinoma antigen NY REN 27 antibody
      • Renal carcinoma antigen NY-REN-27 antibody
      • Rosenthal fiber component antibody
      see all

    图片

    • Western blot - Anti-Alpha B Crystallin antibody [1A7.D5] (ab74441)
      Western blot - Anti-Alpha B Crystallin antibody [1A7.D5] (ab74441)
      All lanes : Anti-Alpha B Crystallin antibody [1A7.D5] (ab74441) at 1/100 dilution

      Lane 1 : AlphaA crystallin protein
      Lane 2 : AlphaB crystallin protein

      Predicted band size: 20 kDa
      Observed band size: 20 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [1A7.D5] (ab74441)
      Immunocytochemistry/ Immunofluorescence - Anti-Alpha B Crystallin antibody [1A7.D5] (ab74441)

      4% formaldehyde-fixed SK-N-BE cells stained for Alpha B Crystallin (green) using ab74441 at 1/100 dilution in ICC/IF.

      Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 dilution for 60 min at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60min room temperature, 5min room temperature. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab74441 (D) Composite.

    文献 (1)

    发表研究结果有使用 ab74441？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab74441 被引用在 1 文献中.

    • Lomiwes D  et al. Small heat shock proteins and toughness in intermediate pHu beef. Meat Sci 95:472-9 (2013). WB ; Cow . PubMed: 23793082

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