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Anti-alpha Adducin (phospho S726)抗体(ab53093)

Submit a review Q&A (3)References (2)
Western blot - Anti-alpha Adducin (phospho S726) antibody (ab53093)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody (ab53093)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody (ab53093)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody (ab53093)

Key features and details

  • Rabbit polyclonal to alpha Adducin (phospho S726)
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-alpha Adducin (phospho S726)抗体
    参阅全部 alpha Adducin 一抗

  • 描述

    兔多克隆抗体to alpha Adducin (phospho S726)

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-P, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Human alpha Adducin aa 650-750 (phospho S726). Swiss-Prot #: P35611/P35612

  • 阳性对照

    • WB: HeLa cells. IHC-P: Human Brain and Human Breast carcinoma tissue. ICC/IF: HeLa cells.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab53093于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/100 - 1/500.
说明
WB
1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
IHC-P
Use at an assay dependent concentration.
ICC/IF
1/100 - 1/500.

靶标

  • 功能

    Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

  • 组织特异性

    Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.

  • 序列相似性

    Belongs to the aldolase class II family. Adducin subfamily.

  • 结构域

    Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

  • 细胞定位

    Cytoplasm > cytoskeleton. Cell membrane.
  • Information by UniProt

  • 数据库链接

    • 别名

      • ADD A antibody
      • ADD1 antibody
      • ADDA antibody
      • ADDA_HUMAN antibody
      • Adducin 1 (alpha) antibody
      • Alpha adducin antibody
      • Alpha-adducin antibody
      • Erythrocyte adducin alpha subunit antibody
      • Erythrocyte adducin subunit alpha antibody
      • MGC3339 antibody
      • MGC44427 antibody
      • OTTHUMP00000151163 antibody
      • OTTHUMP00000151164 antibody
      • OTTHUMP00000151165 antibody
      • OTTHUMP00000218664 antibody
      • OTTHUMP00000218665 antibody
      see all

    图片

    • Western blot - Anti-alpha Adducin (phospho S726) antibody (ab53093)
      Western blot - Anti-alpha Adducin (phospho S726) antibody (ab53093)
      All lanes : Anti-alpha Adducin (phospho S726) antibody (ab53093) at 1/500 dilution

      Lane 1 : HeLa cells treated with Forskolin (40nM 30 mins)
      Lane 2 : HeLa cells treated with Forskolin (40nM 30 mins) and blocked with the phospho peptide.

      Predicted band size: 81 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody (ab53093)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody (ab53093)
      labelled with ab53093, at 1/50 dilution, staining alpha Adducin in paraffin embedded human brain tissue sections by Immunohistochemistry. Tissues had either been treated with (right image) or without (left image) the phosphopeptide.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody (ab53093)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin (phospho S726) antibody (ab53093)

      Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ab53093 at 1/50. The picture on the right is blocked with the phospo peptide. 

    • Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody (ab53093)
      Immunocytochemistry/ Immunofluorescence - Anti-alpha Adducin (phospho S726) antibody (ab53093)

      Immunocytochemistry analysis of HeLa cells, using ab53093 at 1/100 dilution. The picture on the right is blocked with the phospho peptide.

    文献 (2)

    发表研究结果有使用 ab53093？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab53093 被引用在 2 文献中.

    • Ferrer I  et al. Dysregulated protein phosphorylation: A determining condition in the continuum of brain aging and Alzheimer's disease. Brain Pathol 31:e12996 (2021). PubMed: 34218486
    • Tura A  et al. Efficacy of Rho-kinase inhibition in promoting cell survival and reducing reactive gliosis in the rodent retina. Invest Ophthalmol Vis Sci 50:452-61 (2009). PubMed: 18757509

    客户评价及客户问答

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    1-3 of 3 Abreviews or Q&A

    Question


    thank you for your answer.
    However, we think that the interpretation you mentionned does not fit with our results.
    Indeed, we have exactly the same blot with ab51130 and ab53093.
    We thus suspect that adducin antibody is in fact directed against phospho-adducin.
    Could you please provide us at least a small aliquot of antibody against alpha-adducin.

    I hope you can understand it is quite urgent

    Read More
    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab40760.



    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Question


    thank you for your answer.
    However, we think that the interpretation you mentionned does not fit with our results.
    Indeed, we have exactly the same blot with ab51130 and ab53093.
    We thus suspect that adducin antibody is in fact directed against phospho-adducin.
    Could you please provide us at least a small aliquot of antibody against alpha-adducin.

    I hope you can understand it is quite urgent
    best wishes

    Read More
    Answer

    I can send you a replacement vial of an anti-adducin antibody of your choice as we do not stock small sample sizes. So I can process your request, please include your PO number or Abcam order reference number associated with the purchase of ab51130, along with your choice of antibody, in your reply.

    Read More

    Question

    Dear Sir, Madame,
    I order a number of our antibodies at Abcam, and we are usually pleased by their quality, except for one case.
    The following antibodies were obtained:
    - - anti alpha-adducin (rabbit polyclonal, ab51130)
    - - anti phospho-alpha-adducin (Ser 726) (rabbit polyclonal, ab53093).
    Unfortunately they give exactly the same staining pattern in the region of interest (about 100 kDa), as if they were both directed against phosphorylated adducin (see attachment). Due to this fact, it is not possible to use ab51130 antibody as control of equal sample loading, as we planned.
    Please could you send us another antibody against alpha-adducin, which reacts with this protein regardless of phosphorylation state.

    Read More
    Answer

    Thank you for your enquiry and for providing the attached western blot data.

    Here are a few points to consider:

    1. Since a small, non-phosphorylated peptide was used as the immunogen for ab51130, itwill not be reactive with total adducin, only adducin which is not phosphorylated around S726. Phosphorylation at S726 will block biniding of ab51130. So in general this antibody is not a good choice for detecting "total" adducin.

    I am currently checking our anti-adducin products to determine if we have an antibody that could be used as a 'total adducin antibody". I will reply as soon as I hear back from the lab.

    2. As acontrol, consider treating membrane with phosphatase. This would remove any phosphorylation events and ensure the phosphorylation status is known prior to blotting.

    Please see the attached phosphatase treatment protocol as an example.

    3. Sodium pervanadate is a tyrosine phosphatase inhibitor. it should not have any effect on phosphorylation status of Ser726. Perhaps something unexpected is occuring due to addition of inhibitors.

    I hope this is helpful. Please contact me if you have further questions.

    Read More

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