Anti-alpha Adducin抗体(ab51130)
Key features and details
- Rabbit polyclonal to alpha Adducin
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-alpha Adducin抗体
参阅全部 alpha Adducin 一抗 -
描述
兔多克隆抗体to alpha Adducin -
宿主
Rabbit -
特异性
There are 2 isoforms of alpha adducin on the Uniprot protein database page for mouse. The antibody immunogen has high alignment to isoform 1: Isoform 1 80 kDa 100% Isoform 2 69 kDa 30% The aduccin protein is made up of 4 subunits, including the alpha beta and gamma subunits (according to the Uniprot page), Alignment of the immunogen with the mouse beta adducin: http://www.uniprot.org/uniprot/Q9QYB8 isoform 1 100% isoform 2 30% isoform 3 100% And with the mouse gamma adducin: http://www.uniprot.org/uniprot/Q9QYB5 isoform 1 92% Isoform 2 92% The antibody has not been specifically tested for detection of the other subunits, however according to the high alignments, the antibody is likely to detect several isoforms of these beta and gamma subunits. -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthesized non-phosphopeptide derived from human alpha Adducin around the phosphorylation site of serine 726 (T-P-SP-F-L).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51130于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
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ICC/IF | (1) |
Use a concentration of 1 - 5 µg/ml.
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说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa). |
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
靶标
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功能
Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin. -
组织特异性
Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit. -
序列相似性
Belongs to the aldolase class II family. Adducin subfamily. -
结构域
Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region. -
细胞定位
Cytoplasm > cytoskeleton. Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 118 Human
- Entrez Gene: 11518 Mouse
- Entrez Gene: 24170 Rat
- Omim: 102680 Human
- SwissProt: P35611 Human
- SwissProt: Q9QYC0 Mouse
- SwissProt: Q63028 Rat
- Unigene: 183706 Human
see all -
别名
- ADD A antibody
- ADD1 antibody
- ADDA antibody
see all
图片
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All lanes : Anti-alpha Adducin antibody (ab51130)
Lane 1 : Extracts from Hela cells treated
with Forskolin (40nM, 30min) with no peptide
Lane 2 : Extracts from Hela cells treated
with Forskolin (40nM, 30min) with with immunising peptide
Predicted band size: 81 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha Adducin antibody (ab51130)This image shows paraffin-embedded human brain tissue stained with ab51130 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue
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ICC/IF image of ab51130 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51130, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab51130 被引用在 12 文献中.
- Pintacuda G et al. Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders. Cell Genom 3:100250 (2023). PubMed: 36950384
- Moztarzadeh S et al. Lack of adducin impairs the stability of endothelial adherens and tight junctions and may be required for cAMP-Rac1-mediated endothelial barrier stabilization. Sci Rep 12:14940 (2022). PubMed: 36056066
- Cabriel C et al. Combining 3D single molecule localization strategies for reproducible bioimaging. Nat Commun 10:1980 (2019). PubMed: 31040275
- Wang G et al. Structural plasticity of actin-spectrin membrane skeleton and functional role of actin and spectrin in axon degeneration. Elife 8:N/A (2019). PubMed: 31042147
- Gervasi MG et al. The actin cytoskeleton of the mouse sperm flagellum is organized in a helical structure. J Cell Sci 131:N/A (2018). PubMed: 29739876