AB109422

重组Anti-alpha 2 Macroglobulin抗体[EPR4432]

Anti-alpha 2 Macroglobulin antibody [EPR4432]

RabMAb
Recombinant
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(5 Publications)

Rabbit Recombinant Monoclonal alpha 2 Macroglobulin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.

查看别名

CPAMD5, FWP007, A2M, Alpha-2-macroglobulin, Alpha-2-M, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2 Macroglobulin antibody [EPR4432] (AB109422)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 2 Macroglobulin antibody [EPR4432] (AB109422)

ab109422 at 1/500 dilution staining alpha 2 Macroglobulin in paraffin-embedded Human liver by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-alpha 2 Macroglobulin antibody [EPR4432] (AB109422)

All lanes:

Western blot - Anti-alpha 2 Macroglobulin antibody [EPR4432] (ab109422) at 1/1000 dilution

All lanes:

Human plasma lysate at 10 µg

Predicted band size: 163 kDa

false

不同偶联物与剂型 (1)

Carrier free

Anti-alpha 2 Macroglobulin antibody [EPR4432] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4432

亚型

IgG

不含载体蛋白

反应种属

Human

应用

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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产品详情

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

运输条件

Blue Ice

储存信息

Stable for 12 months at -20°C

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-2-macroglobulin also known as alpha-2-M and A2M is a large glycoprotein with a molecular mass of approximately 725 kDa. It is expressed in the liver and is found in high concentrations in the blood plasma. This protein functions mechanically as a broad-spectrum protease inhibitor. It can trap proteases within its structure preventing them from accessing substrates. Additionally alpha-2-macroglobulin can bind to cytokines and growth factors altering their availability and activities.

Biological function summary

Alpha-2-macroglobulin plays an important role in maintaining homeostasis by regulating protease activities and modulating immune system reactions. It does not form a permanent complex but can transiently associate with the proteases and various molecules mentioned. By interacting with proteases it controls processes like coagulation and fibrinolysis and participates in the clearance of proteinases and growth factors from the circulation. It influences cell signaling pathways and inflammatory responses highlighting its significance in maintaining balance within the body.

Pathways

Alpha-2-macroglobulin is an important component in the immune response and coagulation pathways. In the complement system it binds with complement components to regulate their activities. It associates with proteins such as plasmin and thrombin which are important for blood clotting and fibrinolysis. These relationships emphasize the protein's involvement in both protective mechanisms against pathogens and the regulation of blood coagulation and breakdown connecting the immune and hemostatic systems.

Alpha-2-macroglobulin has been linked to Alzheimer's disease and liver diseases. In Alzheimer's altered levels of this protein might affect the amyloid-beta peptide clearance due to its protease inhibition and binding properties. It associates with proteins like lipoprotein receptor-related protein-1 (LRP1) influencing amyloid-beta metabolism. In liver diseases its levels can indicate liver dysfunction or inflammation given its synthesis in the liver and can relate to proteins involved in inflammation such as cytokines. Its modulation of protease activity makes it a critical target in understanding these disorders.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

See full target information A2M

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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 16:3141-3162 PubMed40740229

2025

Alpha-2-Macroglobulin and Signature Genes: Predictive Biomarkers for Prognosis and Immunotherapy in Clear Cell Renal Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Li,Xin Luo,Renyu Zhou,Minting Liu,Guang Wang,Xiaotan Zhang

Nature medicine 31:1604-1616 PubMed40050704

2025

Microglial mechanisms drive amyloid-β clearance in immunized patients with Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lynn van Olst,Brooke Simonton,Alex J Edwards,Anne V Forsyth,Jake Boles,Pouya Jamshidi,Thomas Watson,Nate Shepard,Talia Krainc,Benney Mr Argue,Ziyang Zhang,Joshua Kuruvilla,Lily Camp,Mengwei Li,Hang Xu,Jeanette L Norman,Joshua Cahan,Robert Vassar,Jinmiao Chen,Rudolph J Castellani,James Ar Nicoll,Delphine Boche,David Gate

General physiology and biophysics 43:231-242 PubMed38774923

2024

CCCTC-binding factor suppresses alpha-2-macroglobulin transcription to improve vascular endothelial cell functions in lower extremity arteriosclerosis obliterans.

Applications

Unspecified application

Species

Unspecified reactive species

Tianmin He,Mengqiang You,Huixin Zhu,Peng Chen,Shanhong Fang

Journal of cancer research and clinical oncology 150:163 PubMed38546882

2024

Urocortin-1 promotes colorectal cancer cell migration and proliferation and inhibits apoptosis via inhibition of the p53 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolan Guo,Ya Li,Xiangyu Chen,Binghua Sun,Xiaolan Guo

Frontiers in cell and developmental biology 10:770550 PubMed35300428

2022

An Aging-Related Gene Signature-Based Model for Risk Stratification and Prognosis Prediction in Lung Squamous Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Yu Zhai,Fang-Fang Duan,Si Chen,Jun-Ye Wang,Ze-Rui Zhao,Yi-Zhi Wang,Bing-Yu Rao,Yao-Bin Lin,Hao Long

View all publications

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重组Anti-alpha 2 Macroglobulin抗体[EPR4432]