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AB248454

重组Anti-alpha 2 Macroglobulin抗体[EPR4431] - BSA and Azide free

Anti-alpha 2 Macroglobulin antibody [EPR4431] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal alpha 2 Macroglobulin antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

CPAMD5, FWP007, A2M, Alpha-2-macroglobulin, Alpha-2-M, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5

1 Images
Western blot - Anti-alpha 2 Macroglobulin antibody [EPR4431] - BSA and Azide free (AB248454)
  • WB

Unknown

Western blot - Anti-alpha 2 Macroglobulin antibody [EPR4431] - BSA and Azide free (AB248454)

This data was developed using ab133299, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-alpha 2 Macroglobulin antibody [EPR4431] (<a href='/products/primary-antibodies/alpha-2-macroglobulin-antibody-epr4431-ab133299'>ab133299</a>) at 1/1000 dilution

All lanes:

Human plasma lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 163 kDa

false

不同偶联物与剂型 (1)

  • Unconjugated

    Anti-alpha 2 Macroglobulin antibody [EPR4431]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR4431

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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产品详情

ab248454 is the carrier-free version of ab133299.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
储存信息
Do Not Freeze
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Alpha-2-macroglobulin also known as alpha-2-M and A2M is a large glycoprotein with a molecular mass of approximately 725 kDa. It is expressed in the liver and is found in high concentrations in the blood plasma. This protein functions mechanically as a broad-spectrum protease inhibitor. It can trap proteases within its structure preventing them from accessing substrates. Additionally alpha-2-macroglobulin can bind to cytokines and growth factors altering their availability and activities.
Biological function summary

Alpha-2-macroglobulin plays an important role in maintaining homeostasis by regulating protease activities and modulating immune system reactions. It does not form a permanent complex but can transiently associate with the proteases and various molecules mentioned. By interacting with proteases it controls processes like coagulation and fibrinolysis and participates in the clearance of proteinases and growth factors from the circulation. It influences cell signaling pathways and inflammatory responses highlighting its significance in maintaining balance within the body.

Pathways

Alpha-2-macroglobulin is an important component in the immune response and coagulation pathways. In the complement system it binds with complement components to regulate their activities. It associates with proteins such as plasmin and thrombin which are important for blood clotting and fibrinolysis. These relationships emphasize the protein's involvement in both protective mechanisms against pathogens and the regulation of blood coagulation and breakdown connecting the immune and hemostatic systems.

Alpha-2-macroglobulin has been linked to Alzheimer's disease and liver diseases. In Alzheimer's altered levels of this protein might affect the amyloid-beta peptide clearance due to its protease inhibition and binding properties. It associates with proteins like lipoprotein receptor-related protein-1 (LRP1) influencing amyloid-beta metabolism. In liver diseases its levels can indicate liver dysfunction or inflammation given its synthesis in the liver and can relate to proteins involved in inflammation such as cytokines. Its modulation of protease activity makes it a critical target in understanding these disorders.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
See full target information A2M
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 24:457 PubMed36380881

2022

Suppressive effect of YY1-mediated RGS22 regulation on the proliferation, migration and invasion of pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shou-Ji Cao,Wan-Li Ge,Ling-Dong Meng,Qun Chen,Yi Miao,Kui-Rong Jiang,Jing-Jing Zhang
View all publications
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搭配使用产品

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  • 5
  • 2

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