AB150414

重组Anti-Alpha 2 Antiplasmin抗体[EPSIS17]

Anti-Alpha 2 Antiplasmin antibody [EPSIS17]

BOND RX™ Validated
RabMAb
了解详情

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Monoclonal Alpha 2 Antiplasmin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.

查看别名

AAP, PLI, SERPINF2, Alpha-2-antiplasmin, Alpha-2-AP, Alpha-2-plasmin inhibitor, Serpin F2, Alpha-2-PI

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha 2 Antiplasmin antibody [EPSIS17] (AB150414)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Alpha 2 Antiplasmin antibody [EPSIS17] (AB150414)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Alpha 2 Antiplasmin with ab150414 at 1/150 dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Lab

Western blot - Anti-Alpha 2 Antiplasmin antibody [EPSIS17] (AB150414)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Alpha 2 Antiplasmin antibody [EPSIS17] (ab150414) at 1/10000 dilution

All lanes:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 65 kDa

false

Exposure time: 5s

不同偶联物与剂型 (1)

Carrier free

Anti-Alpha 2 Antiplasmin antibody [EPSIS17] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPSIS17

亚型

IgG

不含载体蛋白

反应种属

Human

应用

WB, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "Heat up to 98°C, below boiling, and then let cool for 10-20 min.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

产品详情

This product has switched from a hybridoma to recombinant production method on 2nd August 2023.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 2 Antiplasmin also known as A2AP or anti-alpha 2 plays an important role in inhibiting the fibrinolytic system by inactivating plasmin. Weighing around 70 kDa this glycoprotein is mainly expressed in the liver and circulates in plasma. Its alternative names include alpha-2-antiplasmin and anti-alpha 2 which highlight its blocking role against excess plasmin action.

Biological function summary

Alpha 2 Antiplasmin functions as the primary inhibitor of plasmin blocking unwanted fibrinolysis. It can form a complex with fibrin which enhances its efficiency in preventing premature fibrin clot dissolution. This regulation of plasmin activity ensures the stability of clots essential for proper wound healing and controlling bleeding.

Pathways

Alpha 2 Antiplasmin engages significantly with the fibrinolysis and coagulation pathways. Its action balances the fibrinolytic process which involves transforming plasminogen to plasmin. Related proteins like plasminogen activator inhibitor also regulate plasmin maintaining equilibrium between clot formation and degradation.

Alpha 2 Antiplasmin has implications in hemorrhagic conditions and thrombotic disorders. Its deficiency might lead to excessive bleeding due to unregulated plasmin activity whilst elevated levels could contribute to thrombotic problems causing harmful clot formation. The interplay with plasminogen and its activation is important in understanding its disease linkages.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

Serine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.

See full target information SERPINF2

style

left-column

文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 31:1219-1234 PubMed38867004

2024

The tRNA Gm18 methyltransferase TARBP1 promotes hepatocellular carcinoma progression via metabolic reprogramming of glutamine.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyan Shi,Yangyi Zhang,Yuci Wang,Jie Wang,Yang Gao,Ruiqi Wang,Liyong Wang,Minggang Xiong,Yanlan Cao,Ningjing Ou,Qi Liu,Honghui Ma,Jiabin Cai,Hao Chen

Proceedings of the National Academy of Sciences of 115:E9687-E9696 PubMed30254165

2018

Blood-derived plasminogen drives brain inflammation and plaque deposition in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah K Baker,Zu-Lin Chen,Erin H Norris,Alexey S Revenko,A Robert MacLeod,Sidney Strickland

View all publications

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Alpha 2 Antiplasmin抗体[EPSIS17]