Name: Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3]
SKU: ab2872
Rating: 5 (3 reviews)

Product price, shipping and contact information

Currently unavailable

抱歉，我们目前无法显示此内容。

请联系我们以完成下单，或者之后再尝试访问。

正在加载规格和价格。。。

Abpromise

产品质量保证，客户专家支持，

点击获取更多

Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

Key features and details

Mouse monoclonal [M8-P1-A3] to alpha 1 Sodium Potassium ATPase
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Reacts with: Human
Isotype: IgG1

选择批间可重复性更高的重组抗体

Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (ab76020)

研究可靠 —— 各批次间结果一致且可重复
长期批量供应 —— 采用重组技术，可实现快速生产
首次实验即可成功 —— 经过大量验证确认了特异性
符合伦理标准 —— 产品不含动物成分

产品名称

Anti-alpha 1 Sodium Potassium ATPase抗体[M8-P1-A3]
参阅全部 alpha 1 Sodium Potassium ATPase 一抗
描述

小鼠单克隆抗体[M8-P1-A3] to alpha 1 Sodium Potassium ATPase
宿主

Mouse
经测试应用

适用于: WB, IHC-P, ICC/IF, Flow Cytmore details
种属反应性

与反应: Human
免疫原

Full length protein. This information is proprietary to Abcam and/or its suppliers.
表位

This antibody recognizes an epitope between amino acid residues 496-506 of lamb kidney sodium/potassium ATPase.
阳性对照
- WB: canine kidney extract
常规说明

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液

Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

M8-P1-A3
同种型

IgG1
研究领域

The Abpromise guarantee

Abpromise™承诺保证使用ab2872于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB	(1)	1/200 - 1/2000. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).
IHC-P	(2)	1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/50 - 1/200.
Flow Cyt		1/20 - 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

说明
WB 1/200 - 1/2000. Detects a band of approximately 100 kDa (predicted molecular weight: 110 kDa).
IHC-P 1/100 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/200.
Flow Cyt 1/20 - 1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

功能

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
序列相似性

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
翻译后修饰

Phosphorylation on Tyr-10 modulates pumping activity.
细胞定位

Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Target information above from: UniProt accession P05023 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 476 Human
- Omim: 182310 Human
- SwissProt: P05023 Human
- Unigene: 371889 Human
别名
- A1A1 antibody
- AT1A1 antibody
- AT1A1_HUMAN antibody
- ATP1A1 antibody
- Atpa-1 antibody
- ATPase Na+/K+ transporting alpha 1 polypeptide antibody
- ATPase Na+/K+ transporting subunit alpha 1 antibody
- BC010319 antibody
- EC 3.6.3.9 antibody
- MGC3285 antibody
- MGC38419 antibody
- MGC51750 antibody
- Na K ATPase alpha A catalytic polypeptide antibody
- Na K ATPase catalytic subunit alpha A protein antibody
- Na(+)/K(+) ATPase 1 antibody
- Na(+)/K(+) ATPase alpha-1 subunit antibody
- Na+, K+ ATPase alpha subunit antibody
- Na+/K+ ATPase alpha 1 subunit antibody
- Na+/K+ ATPase 1 antibody
- Na,K ATPase alpha 1 subunit antibody
- Nkaa1b antibody
- Sodium potassium ATPase alpha 1 polypeptide antibody
- Sodium pump 1 antibody
- Sodium pump subunit alpha-1 antibody
- sodium-potassium ATPase catalytic subunit alpha-1 antibody
- Sodium/potassium-transporting ATPase subunit alpha-1 antibody
see all

Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

All lanes : Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872) at 1/1000 dilution

Lane 1 : Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma cell line) whole cell lysate

Lysates/proteins at 30 µg per lane.

Secondary
All lanes : Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

Predicted band size: 110 kDa

Samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® 2 Dry Blotting System.

Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit.
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

Immunocytochemistry/Immunofluorescence analysis of alpha 1 Sodium Potassium ATPase (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 0.3% BSA for 15 minutes at room temperature. Cells were incubated with ab2872 (1:100) for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (1:500) for 30 minutes at room temperature. F-actin (red) was stained with DyLight 594 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)This image is courtesy of an anonymous Abreview

ab2872 staining pig hepatocyte tissue sections by IHC-P. The section was fixed with Bouins and subjected to heat mediated antigen retrieval (at pH 9) prior to incubating with the primary antibody, diluted 1/2000, for 1 hour at 20°C. A Cy3® conjugated goat anti-mouse IgG antibody was used as the secondary.
See Abreview
Flow Cytometry - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

Overlay histogram showing HEK293 cells stained with ab2872 (red line). The cells were fixed with 100% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2872, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 4% paraformaldehyde used under the same conditions.

Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:50 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-1 ab2872 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human kidney tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-1 ab2872 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon carcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Sodium/Potassium ATPase alpha-1 ab2872 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
Western blot - Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872)

All lanes : Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3] (ab2872) at 1/500 dilution

Lane 1 : Human brain normal tissue lysate - membrane extract (ab29456)
Lane 2 : Human testis tissue lysate - total protein (ab30257)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 110 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 25 kDa, 70 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 8 minutes

The 100 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to alpha 1 Sodium Potassium ATPase.

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab2872？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab2872 被引用在 21 文献中.

Zhang SH et al. A CASPR1-ATP1B3 protein interaction modulates plasma membrane localization of Na+/K+-ATPase in brain microvascular endothelial cells. J Biol Chem 294:6375-6386 (2019). PubMed: 30792309
Zhang F et al. Molecular mechanisms of caffeine-mediated intestinal epithelial ion transports. Br J Pharmacol 176:1700-1716 (2019). PubMed: 30808064
He Y et al. Combined assessment of low PGRMC1/positive ATP1A1 levels has enhanced prognostic value for renal cell carcinoma. Oncol Rep 40:1467-1476 (2018). PubMed: 30015972
Zhang D et al. Downregulation of ATP1A1 promotes cancer development in renal cell carcinoma. Clin Proteomics 14:15 (2017). WB, IHC ; Human . PubMed: 28484360
Zhu W et al. Effect of docosahexaenoic acid on traumatic brain injury in rats. Exp Ther Med 14:4411-4416 (2017). PubMed: 29075341

View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A

提交评价提交问题

1-6 of 6 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Hepatocytes)

Specification

Hepatocytes

Fixative

Bouins

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 9,0

Permeabilization

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 04 2008

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Pig Tissue sections (Hepatocytes)

Specification

Hepatocytes

Fixative

Bouins

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH 9,0

Permeabilization

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Apr 03 2008

Application

Western blot

Sample

Human Cell lysate - whole cell (Glioma)

Specification

Glioma

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. Amy Weaver

Verified customer

提交于 Dec 12 2005

Question

Thank you very much for kindly answering my questions and i would like to know how long does it takes to have ab2872 antibody with new lot number. I would like to have this antibody with new lot number.

Abcam community

Verified customer

Asked on May 13 2005

Answer

Thank you for your reply. We are going to send you a new batch as soon as we receive it. This is to let you know that I have placed a new order for you - one vial of ab2872 free of charge. For your information your new order number is: 83747. Thank you for your patience!

Abcam Scientific Support

回复于 May 17 2005

Question

1.actually the secondary antibody which i am using is anti-mouse IgG, Horseradish Peroxidase linked whole antibody(from sheep) from amersham. 2. i have been doing western blots from past 5 years. 3.i am following the same protocol for all other antibodies even your other abcam antibodies, i never experienced this problem. 4.As per my knowledge the incubation time doesn't matter either overnight or 2 hours. 5. i don't think reducing the boiling time will make difference. could you please replace with the new antibody.

Abcam community

Verified customer

Asked on May 11 2005

Answer

Thank you for getting back to me and providing more information about your Western blot protocol. We can offer you a new vial as a free of charge replacement; however it is from the exatly the same batch. Unfortunately, we do not have different batch in our stock at the moment. We would like to suggest loading less protein than 45 ug per lane, the optimal loading should be 20-30 ug per lane. We do understand and appreciate your experience doing Western blot assay. Using standard protocol is useful; however each target protein and antibody needs optimization. Boiling can make a difference and if the samples are over-boiled, it can change the detection of the target protein. Try to boil the samples for 3 or max 5 min. Please so let me know if you would like to get the vial.

Abcam Scientific Support

回复于 May 12 2005

Question

BATCH NUMBER 90665 ORDER NUMBER 66788 DESCRIPTION OF THE PROBLEM Multiple bands SAMPLE rat np cell lysates and we couldn't see bands in rat tissues like kidney, cartilage, liver and heart PRIMARY ANTIBODY 1-250 dilution as recommended in protocol of ab2872. incubated for O/N at 4 degrees. 3 times wash with 1X TBST - 10 min/wash DETECTION METHOD ECL advanced POSITIVE AND NEGATIVE CONTROLS USED I used for rat kidney tissue ANTIBODY STORAGE CONDITIONS -20 SAMPLE PREPARATION i lysis the cells from MPER buffer and i used protease cocktail. I heated the samples with 5x sample buffer for 7-10 min. AMOUNT OF PROTEIN LOADED 45ug ELECTROPHORESIS/GEL CONDITIONS SDS page gel TRANSFER AND BLOCKING CONDITIONS Transfer - ready to use buffer from biorad, 70 volts for 2 hours at 4 degrees. 5% non fat dry milk in 1X TBST SECONDARY ANTIBODY 1-2000 dilution with HRP mouse monoclonal from amersham, incubated at RT for 1 1/2 hour. 3 times wash with 1X TBST - 10 min/wash HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 3 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? first time i used rat tissue lysates, i see multiple bands and then i tried with rat NP cell lysates. I got multiple bands again ADDITIONAL NOTES If u want i can send the blot which i ran. i never got these multiple bands with ur antibodies which i used before like HSP-70(very clean and good antibody). i am getting the bands only with this particular antibody. I would like request you to replace this antibody with new batch of same antibody

Abcam community

Verified customer

Asked on May 09 2005

Answer

Thank you for contacting us regarding ab2872 and for taking your time to fill in the on-line Questionnaire. We are very sorry to hear that you are experiencing problem with this antibody. As we understand from your e-mail, you have used HRP mouse monoclonal as secondary antibody. Ab2872 is Mouse monoclonal antibody to alpha 1 Sodium Potassium ATPase so the compatible secondary antibody should be raised in a different species rather than mouse. Could you please specify again in which species the secondary antibody was raised? We would suggest loading 25-30 ug protein per lane, 45 ug is probably a bit too much. Try to boil the samples for 3-5 min but not longer than 5 min and incubate the membrane for 1 or 2 hrs instead of overnight. Should you still have problem with this antibody, then please do not hesitate to contact us again.

Abcam Scientific Support

回复于 May 10 2005

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

表位

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Compatible Secondaries

Conjugation kits

Isotype control

应用

The Abpromise guarantee

靶标

功能

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (21)

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3]

Western blot abreview for Anti-alpha 1 Sodium Potassium ATPase antibody [M8-P1-A3]