重组Anti-alpha 1 Fetoprotein抗体[SP154] (ab130748)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [SP154] (ab130748)](https://www.abcam.cn/ps/products/130/ab130748/Images/ab130748-6-Antialpha-1-Fetoprotein-antibody-SP154-IHC-Human-placenta.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [SP154] (ab130748)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP154] to alpha 1 Fetoprotein
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-alpha 1 Fetoprotein抗体[SP154]
参阅全部 alpha 1 Fetoprotein 一抗 -
描述
兔单克隆抗体[SP154] to alpha 1 Fetoprotein -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Human alpha 1 Fetoprotein.
Database link: P02771 -
阳性对照
- IHC-P: Hepatocellular carcinoma, and Human placenta tissue; WB: HepG2 cell lysate; Flow Cyt: HepG2 cells; ICC: HepG2 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
纯度
Protein A/G purified -
纯化说明
Purified from TCS by protein A/G. -
克隆
单克隆 -
克隆编号
SP154 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab130748于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/100. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). Incubate for 1 hour at room temperature.
|
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| IHC-P |
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate for 10 minutes at room temperature.
|
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| ICC/IF |
1/100.
|
|
| Flow Cyt |
1/240.
|
| 说明 |
|---|
|
WB
1/100. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa). Incubate for 1 hour at room temperature. |
|
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate for 10 minutes at room temperature. |
|
ICC/IF
1/100. |
|
Flow Cyt
1/240. |
靶标
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功能
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
组织特异性
Plasma. Synthesized by the fetal liver and yolk sac. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
发展阶段
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
翻译后修饰
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
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别名
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [SP154] (ab130748)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human placenta tissue sections labeling alpha 1 Fetoprotein with ab130748 at 1/50 dilution (4.98 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [SP154] (ab130748)](https://www.abcam.cn/ps/products/130/ab130748/Images/ab130748-5-Antialpha-1-Fetoprotein-antibody-SP154-IHC-Human-hepatocellular-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [SP154] (ab130748)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling alpha 1 Fetoprotein with ab130748 at 1/50 dilution (4.98 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. -
![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [SP154] (ab130748)](https://www.abcam.cn/ps/products/130/ab130748/Images/ab130748-338483-anti-alpha-1-fetoprotein-antibody-sp154-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [SP154] (ab130748)Immunocytochemistry/ Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma epithelial cell) cells labeling alpha 1 Fetoprotein with purified ab130748 at 1:100 (2.5 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [SP154] (ab130748)](https://www.abcam.cn/ps/products/130/ab130748/Images/ab130748-338505-anti-alpha-1-fetoprotein-antibody-sp154-flow-cytometry.jpg)
Flow Cytometry - Anti-alpha 1 Fetoprotein antibody [SP154] (ab130748)Flow Cytometry analysis of HepG2(Human hepatocellular carcinoma epithelial cell) cells labeling alpha 1 Fetoprotein with purified ab130748 at 1:240 dilution (1.04 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue. -
![Western blot - Anti-alpha 1 Fetoprotein antibody [SP154] (ab130748)](https://www.abcam.cn/ps/products/130/ab130748/Images/ab130748-145084-anti-alpha-1-fetoprotein-antibody-sp154-western-blot.jpg)
Western blot - Anti-alpha 1 Fetoprotein antibody [SP154] (ab130748)Anti-alpha 1 Fetoprotein antibody [SP154] (ab130748) at 1/100 dilution + HepG2 cell lysate
Predicted band size: 68 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
![Anti-alpha 1 Fetoprotein antibody [SP154] (ab130748)](https://www.abcam.cn/ps/products/130/ab130748/Images/ab130748-9-benefits-of-recombinant-antibodies.png)
Anti-alpha 1 Fetoprotein antibody [SP154] (ab130748)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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