重组Anti-alpha 1 Fetoprotein抗体[EPR19240-133] - BSA and Azide free (ab226488)
![Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] - BSA and Azide free (ab226488)](https://www.abcam.cn/ps/products/226/ab226488/Images/ab226488-2-ab199054287579199054IP.jpg "Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] - BSA and Azide free (ab226488)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19240-133] to alpha 1 Fetoprotein - BSA and Azide free
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse
Related conjugates and formulations
概述
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产品名称
Anti-alpha 1 Fetoprotein抗体[EPR19240-133] - BSA and Azide free
参阅全部 alpha 1 Fetoprotein 一抗 -
描述
兔单克隆抗体[EPR19240-133] to alpha 1 Fetoprotein - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IPmore details -
种属反应性
与反应: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: Hepa1-6 cells.
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常规说明
ab226488 is the carrier-free version of ab199054.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR19240-133 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226488于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa).
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| ICC/IF |
Use at an assay dependent concentration.
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| IP |
Use at an assay dependent concentration.
|
| 说明 |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 67 kDa). |
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ICC/IF
Use at an assay dependent concentration. |
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IP
Use at an assay dependent concentration. |
靶标
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功能
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
组织特异性
Plasma. Synthesized by the fetal liver and yolk sac. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
发展阶段
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
翻译后修饰
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 11576 Mouse
- SwissProt: P02772 Mouse
- Unigene: 358570 Mouse
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别名
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
see all
图片
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Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] - BSA and Azide free (ab226488)
alpha 1 Fetoprotein was immunoprecipitated from 0.35 mg of mouse placenta lysate with ab199054 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199054 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: Mouse placenta lysate 10 µg (Input).
Lane 2: ab199054 IP in mouse placenta lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199054 in mouse placenta lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199054).
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![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] - BSA and Azide free (ab226488)](https://www.abcam.cn/ps/products/226/ab226488/Images/ab226488-287584-226488IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPR19240-133] - BSA and Azide free (ab226488)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling alpha 1 Fetoprotein with ab199054 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cells.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199054).
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![Anti-alpha 1 Fetoprotein antibody [EPR19240-133] - BSA and Azide free (ab226488)](https://www.abcam.cn/ps/products/226/ab226488/Images/ab226488-3-benefits-of-recombinant-antibodies.png)
Anti-alpha 1 Fetoprotein antibody [EPR19240-133] - BSA and Azide free (ab226488)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab226488 尚未被引用在任何文献中。
