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重组RabMAb

重组Anti-alpha 1 Fetoprotein抗体[EPAFP61] - BSA and Azide free (ab271896)

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Western blot - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)
  • Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)
  • Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)
  • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)
  • Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPAFP61] to alpha 1 Fetoprotein - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, IP, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Unconjugated

概述

  • 产品名称

    Anti-alpha 1 Fetoprotein抗体[EPAFP61] - BSA and Azide free
    参阅全部 alpha 1 Fetoprotein 一抗

  • 描述

    兔单克隆抗体[EPAFP61] to alpha 1 Fetoprotein - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IHC-P, IP, ICC/IFmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant full length protein corresponding to Human alpha 1 Fetoprotein.
    Database link: P02771

  • 阳性对照

    • Flow Cyt (intra): HepG2 cells; IP: HepG2 whole cell lysate; ICC/IF: HepG2 cells; IHC-P: Human liver cancer tissue; WB: HepG2 whole cell lysate.

  • 常规说明

    ab271896 is the carrier-free version of ab133617.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab271896于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Heat up to 98°C, below boiling, and then let cool for 10-20 min. 
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
说明
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Heat up to 98°C, below boiling, and then let cool for 10-20 min. 
IP
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.

靶标

  • 功能

    Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

  • 组织特异性

    Plasma. Synthesized by the fetal liver and yolk sac.

  • 序列相似性

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.

  • 发展阶段

    Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.

  • 翻译后修饰

    Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
    Sulfated.

  • 细胞定位

    Secreted.
  • Information by UniProt

  • 数据库链接

    • 别名

      • Afp antibody
      • AFPD antibody
      • Alpha fetoglobulin antibody
      • Alpha fetoprotein antibody
      • Alpha fetoprotein precursor antibody
      • Alpha-1-fetoprotein antibody
      • Alpha-fetoglobulin antibody
      • Alpha-fetoprotein antibody
      • alpha-fetoprotein, Hereditary persistence of, included antibody
      • FETA antibody
      • FETA_HUMAN antibody
      • Hereditary persistence of alpha fetoprotein antibody
      • HPAFP antibody
      see all

    图片

    • Western blot - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)
      Western blot - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)
      Anti-alpha 1 Fetoprotein antibody [EPAFP61] (ab133617) at 1/10000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 68 kDa



      This data was developed using ab133617, the same antibody clone in a different buffer formulation.

    • Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)
      Immunoprecipitation - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)

      This data was developed using ab133617, the same antibody clone in a different buffer formulation.
      Purified ab133617 at 1/20 dilution (0.5µg) immunoprecipitating alpha 1 Fetoprotein in HepG2 whole cell lysate.
      Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10µg
      Lane 2 (+): ab133617 + HepG2 whole cell lysate.
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab133617 in HepG2 whole cell lysate.
      VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
      Blocking Buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM/TBST.
      Observed band size: 68 kDa

      Lower bands could be caspase-dependent cleavage fragments. (PMID: 31811908)

    • Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)
      Flow Cytometry (Intracellular) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)

      This data was developed using ab133617, the same antibody clone in a different buffer formulation. Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling alpha 1 Fetoprotein with Purified ab133617 at 1/20 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

       

    • Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)
      Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)
      This data was developed using ab133617, the same antibody clone in a different buffer formulation.
      Immunocytochemistry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling alpha 1 Fetoprotein with Purified ab133617 at 1/250 dilution (0.52 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)
      This data was developed using ab133617, the same antibody clone in a different buffer formulation.
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue sections labeling alpha 1 Fetoprotein with Purified ab133617 at 1/100 dilution (1.3 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)
      Anti-alpha 1 Fetoprotein antibody [EPAFP61] - BSA and Azide free (ab271896)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (0)

    发表研究结果有使用 ab271896？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab271896 尚未被引用在任何文献中。

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