Anti-alpha 1 Fetoprotein抗体[AFP-01] (ab3980)
![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)](https://www.abcam.cn/ps/products/3/ab3980/Images/ab3980-8451-anti-alpha-1-fetoprotein-antibody-afp-01-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)")
Key features and details
- Mouse monoclonal [AFP-01] to alpha 1 Fetoprotein
- Suitable for: WB, Sandwich ELISA, ELISA, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-alpha 1 Fetoprotein抗体[AFP-01]
参阅全部 alpha 1 Fetoprotein 一抗 -
描述
小鼠单克隆抗体[AFP-01] to alpha 1 Fetoprotein -
宿主
Mouse -
特异性
The antibody reacts with human alpha-Fetoprotein (AFP), an oncofetal antigen of 70 kDa. AFP is a major fetal plasma protein, but is not present in healthy adult tissues. Elevated AFP concentrations in adult plasma may be an early marker of hepatocellular carcinoma or teratoblastoma, while high concentrations in amniotic fluid may indicate severe congenital defects of a fetus. -
经测试应用
适用于: WB, Sandwich ELISA, ELISA, ICC/IF, IPmore details -
种属反应性
与反应: Human -
免疫原
Full length native protein (purified) corresponding to Human alpha 1 Fetoprotein.
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阳性对照
- Hep G2 human hepatocellular carcinoma cell line Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE sample buffer.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Proprietary Purification -
纯化说明
Purified by precipitation and chromatography -
克隆
单克隆 -
克隆编号
AFP-01 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
unknown -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
Use at an assay dependent concentration.
|
| Sandwich ELISA |
Use at an assay dependent concentration.
|
|
| ELISA |
Use at an assay dependent concentration.
|
|
| ICC/IF | (2) |
Use a concentration of 5 µg/ml.
|
| IP |
Use at an assay dependent concentration.
Use at an assay dependant dilution. Interaction of the antibody with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions. |
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. |
|
Sandwich ELISA
Use at an assay dependent concentration. |
|
ELISA
Use at an assay dependent concentration. |
|
ICC/IF
Use a concentration of 5 µg/ml. |
|
IP
Use at an assay dependent concentration. Use at an assay dependant dilution. Interaction of the antibody with AFP is dependent on the presence of calcium ions (strongly inhibited by chelating agents). Such characteristics of the antibody can be exploited for immunoaffinity purification of APF under mild elution conditions. |
靶标
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功能
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
组织特异性
Plasma. Synthesized by the fetal liver and yolk sac. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
发展阶段
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
翻译后修饰
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
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别名
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [AFP-01] (ab3980)
ICC/IF image of ab3980 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3980, 5µg/ml) overnight at +4°C. The secondary antibody (green)ÿwas Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
数据表及文件
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SDS download
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Datasheet download
文献 (34)
ab3980 被引用在 34 文献中.
- Salikhova D et al. Therapeutic Effects of hiPSC-Derived Glial and Neuronal Progenitor Cells-Conditioned Medium in Experimental Ischemic Stroke in Rats. Int J Mol Sci 22:N/A (2021). PubMed: 33946667
- Akbari S et al. LGR5/R-Spo1/Wnt3a axis promotes stemness and aggressive phenotype in hepatoblast-like hepatocellular carcinoma cell lines. Cell Signal 82:109972 (2021). PubMed: 33684507
- Meseguer-Ripolles J et al. Dimethyl fumarate reduces hepatocyte senescence following paracetamol exposure. iScience 24:102552 (2021). PubMed: 34151225
- Hogrebe NJ et al. Targeting the cytoskeleton to direct pancreatic differentiation of human pluripotent stem cells. Nat Biotechnol 38:460-470 (2020). PubMed: 32094658
- Tongkobpetch S et al. Generation of two human iPSC lines (MDCUi001-A and MDCUi001-B) from dermal fibroblasts of a Thai patient with X-linked osteogenesis imperfecta using integration-free Sendai virus. Stem Cell Res 39:101493 (2019). PubMed: 31326747