Anti-alpha 1 Fetoprotein抗体[1G7] (ab54745)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [1G7] (ab54745)](https://www.abcam.cn/ps/products/54/ab54745/Images/ab54745-67744-anti-alpha-1-fetoprotein-antibody-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [1G7] (ab54745)")
Key features and details
- Mouse monoclonal [1G7] to alpha 1 Fetoprotein
- Suitable for: WB, IHC-P, Sandwich ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-alpha 1 Fetoprotein抗体[1G7]
参阅全部 alpha 1 Fetoprotein 一抗 -
描述
小鼠单克隆抗体[1G7] to alpha 1 Fetoprotein -
宿主
Mouse -
经测试应用
适用于: WB, IHC-P, Sandwich ELISA, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to amino acids 500-610 of Human alpha 1 Fetoprotein
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常规说明
This product was changed from ascites to tissue culture supernatant on 13 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40 -
Concentration information loading... -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
1G7 -
同种型
IgG -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab54745于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 69 kDa.
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| IHC-P |
Use at an assay dependent concentration.
|
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| Sandwich ELISA |
Use at an assay dependent concentration.
For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab8201 as Detection. |
|
| ICC/IF |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 69 kDa. |
|
IHC-P
Use at an assay dependent concentration. |
|
Sandwich ELISA
Use at an assay dependent concentration. For sandwich ELISA, use this antibody as Capture at 1µg/ml with ab8201 as Detection. |
|
ICC/IF
Use at an assay dependent concentration. |
靶标
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功能
Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. -
组织特异性
Plasma. Synthesized by the fetal liver and yolk sac. -
序列相似性
Belongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
发展阶段
Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma. -
翻译后修饰
Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.
Sulfated. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 174 Human
- Omim: 104150 Human
- SwissProt: P02771 Human
- Unigene: 518808 Human
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别名
- Afp antibody
- AFPD antibody
- Alpha fetoglobulin antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha 1 Fetoprotein antibody [1G7] (ab54745)
alpha 1 Fetoprotein antibody (ab54745) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human tonsil.
This image was generated using the ascites version of the product.
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![Western blot - Anti-alpha 1 Fetoprotein antibody [1G7] (ab54745)](https://www.abcam.cn/ps/products/54/ab54745/Images/ab54745-67743-anti-alpha-1-fetoprotein-antibody-western-blot.jpg)
Western blot - Anti-alpha 1 Fetoprotein antibody [1G7] (ab54745)alpha 1 Fetoprotein antibody (ab54745) at 1ug/lane + HepG2 cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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![Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [1G7] (ab54745)](https://www.abcam.cn/ps/products/54/ab54745/Images/ab54745-67742-anti-alpha-1-fetoprotein-antibody-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-alpha 1 Fetoprotein antibody [1G7] (ab54745)ab54745 at 10 ug/ml staining alpha Fetoprotein in human HeLa cells by Immunocytochemistry / Immunofluorescence.
This image was generated using the ascites version of the product.
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![Sandwich ELISA - Anti-alpha 1 Fetoprotein antibody [1G7] (ab54745)](https://www.abcam.cn/ps/products/54/ab54745/Images/ab54745-67741-anti-alpha-1-fetoprotein-antibody-sandwich-elisa.jpg)
Sandwich ELISA - Anti-alpha 1 Fetoprotein antibody [1G7] (ab54745)Standard Curve for Alpha 1 Fetoprotein dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal to alpha 1 Fetoprotein (ab54745) at 1ug/ml and Detector Antibody Rabbit polyclonal to alpha 1 Fetoprotein (ab8201) at 0.1ug/ml
This image was generated using the ascites version of the product.
实验方案
数据表及文件
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Datasheet download
文献 (3)
ab54745 被引用在 3 文献中.
- Er E et al. Metal Nanoparticles/MoS2 Surface-Enhanced Raman Scattering-Based Sandwich Immunoassay for a-Fetoprotein Detection. ACS Appl Mater Interfaces 13:8823-8831 (2021). PubMed: 33583183
- Lee NE et al. Aglycosylated antibody-producing mice for aglycosylated antibody-lectin coupled immunoassay for the quantification of tumor markers (ALIQUAT). Commun Biol 3:636 (2020). PubMed: 33128033
- Maruyama S et al. Inhibition of apoptosis by miR-122-5p in a-fetoprotein-producing gastric cancer. Oncol Rep 41:2595-2600 (2019). PubMed: 30816512
