重组Anti-alpha 1 Antitrypsin抗体[EPSISR16] - BSA and Azide free (ab226145)
![Western blot - Anti-alpha 1 Antitrypsin antibody [EPSISR16] - BSA and Azide free (ab226145)](https://www.abcam.cn/ps/products/226/ab226145/Images/ab226145-398148-anti-alpha-1-antitrypsin-antibody-epsisr16-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-alpha 1 Antitrypsin antibody [EPSISR16] - BSA and Azide free (ab226145)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPSISR16] to alpha 1 Antitrypsin - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-alpha 1 Antitrypsin抗体[EPSISR16] - BSA and Azide free
参阅全部 alpha 1 Antitrypsin 一抗 -
描述
兔单克隆抗体[EPSISR16] to alpha 1 Antitrypsin - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details
不适用于: IHC-P -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: Human platelet, fetal kidney and plasma lysates. IP: Human plasma lysate.
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常规说明
ab226145 is the carrier-free version of ab133642.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPSISR16 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab226145于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
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| IP |
Use at an assay dependent concentration.
|
| 说明 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 46 kDa. |
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IP
Use at an assay dependent concentration. |
靶标
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功能
Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.
Short peptide from AAT: reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). -
组织特异性
Ubiquitous. Expressed in leukocytes and plasma. -
疾病相关
Alpha-1-antitrypsin deficiency -
序列相似性
Belongs to the serpin family. -
结构域
The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable. -
翻译后修饰
N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)[Man(alpha1-3)]Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant.
Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. -
细胞定位
Secreted. Endoplasmic reticulum. The S and Z allele are not secreted effectively and accumulate intracellularly in the endoplasmic reticulum and Secreted, extracellular space, extracellular matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 5265 Human
- Omim: 107400 Human
- SwissProt: P01009 Human
- Unigene: 525557 Human
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别名
- A1A antibody
- A1AT antibody
- A1AT_HUMAN antibody
see all
图片
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Western blot - Anti-alpha 1 Antitrypsin antibody [EPSISR16] - BSA and Azide free (ab226145)All lanes : Anti-alpha 1 Antitrypsin antibody [EPSISR16] (ab133642) at 1/1000 dilution
Lane 1 : Human platelet lysate
Lane 2 : Fetal kidney lysate
Lane 3 : Human plasma lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDaThis data was developed using ab133642, the same antibody clone in a different buffer formulation.
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![Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [EPSISR16] - BSA and Azide free (ab226145)](https://www.abcam.cn/ps/products/226/ab226145/Images/ab226145-3-anti-alpha-1-antitrypsin-antibody-epsisr16-immunoprecipitation-plasma-human.jpg)
Immunoprecipitation - Anti-alpha 1 Antitrypsin antibody [EPSISR16] - BSA and Azide free (ab226145)This data was developed using ab133642, the same antibody clone in a different buffer formulation.
alpha 1 Antitrypsin was immunoprecipitated from 0.35 mg Human plasma 10 µg with ab133642 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.Lane 1: Human plasma 10 µg
Lane 2: abab133642 IP in Human plasma
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab133642 in Human plasma
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-alpha 1 Antitrypsin antibody [EPSISR16] - BSA and Azide free (ab226145)](https://www.abcam.cn/ps/products/226/ab226145/Images/ab226145-2-benefits-of-recombinant-antibodies.png)
Anti-alpha 1 Antitrypsin antibody [EPSISR16] - BSA and Azide free (ab226145)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
文献 (0)
ab226145 尚未被引用在任何文献中。
